I read Clinical Standards and Laboratory Institute standards where they permit use of ethanol/methanol/acetone for some antibiotics which can't be dissolve by water or DMSO. If some antibiotics can dissolve by this process, is it okay to use this alcoholic compounds to prepare plant extract stock solution? I haven't found any standards for plant extracts to dissolve. Some article prefer DMSO and mechanical instruments but have no proper standards.
Thanks for your co-operation.
Yes.
This will depend on what you mean by "plant extract". Is it the powdered whole plant or is it actually an extract?
Because, most times, plant extracts are done using various solvents. Water, hot/cold, ethanol, acid, etc. And depending on what is used, the compositions of the extracts are different. This is a common technique to find /discover the active compounds/protein/etc from plants. (Mostly used in the neutroceutical field.
Actually I mean how to prepare plant stock solution for broth micro-dilution assay. After using drier/rotary evaporator appropriate solvent necessary for stock solution preparation. My concern is, whether alcoholic compounds can be used as solvent for stock solution preparation. As we all know 100% alcoholic compounds have/ may have anti-bacterial effects. Though CLSI permits standard limits of these solvents. @Shen-An Hwang
Ah...I got it now.
The answer is still yes.
When you perform the micro-dilution assay, for your control, you just add the same volume of the solvent without the extract.
The other thing you would have to worry about is that the "extract" or 'antibiotic" will still stay in solution after adding it to the broth culture. Depending on what the extract is... it may dissolve in the solvent but as soon as you dilute that solvent down by introducing it to broth, it may preciptate completely back out.
Please check chemicals characteristics. Ethanol, acetone can inactivate several antibiotics. Go to Google and type CLSI free. Then look for M100 document and in the last part (almost) you can see which dissolvent you can use.
To prepare the plant extracts containing all active compounds, it is possible to use organic solvents at a concentration of 70 per cent with 30 per cent water to ensure the melting of all active compounds. After preparation of the extract in the form of powder, the required concentrations are prepared with the extract of the extract in DMSO and you can use the same solvent, taking into account the activity of the solvent against microorganisms
There is really no hard and fast rule on this. However it is more advisable to use 20, 50 or 70% organic solvent in water provided you do a preliminary test to determine the solubility of the substance in the solvent mix. The less the volume of organic solvent in the mixture, the better. In addition, use your chosen solvent mix as part of the control.
Yes you can use alcohol or any other solvent but that too only to dissolve the extract or any other compound but final dilutions you should have to prepare by diluting it with water.
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