Hi, I am trying to dissolve pure reagent grade Quercetin into cell culture medium (DMEM+sodium bicarbonate+antibiotics) but it clearly does not form a solution. I've found that DMSO might serve but high DMSO concentrations are toxic for cells. Does anyone have found a non-cytotoxic method for dissolving quercetin? Thanks in advance.
We tested quercetin in cells. You may dissolve it at first in DMSO (at 20 mM or 10 mM conc.), then before experiments in cells you should dilute DMSO solution in cell culture medium. Usually up to 0.5% of DMSO is OK, just do not forget to add the same DMSO conc. into the medium for negative control.
You can try to dissolve it in pure ethanol to get 20uM or 10 uM concetration. I am diung it with curcumin. But remember to make fresh stock every two months, cos freeze-thaw cycle can lead to degradation of quercetin.
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