After running RT-PCR for beta actin i got curves after 20 cycles. is this right? Is it mandatory that the curves should come within 20 cycles?

Aleena Varughese @Aleena-Varughese

07 December 2023 0 1K Report

what is the reason behind this?is it due to the cDNA containation?

usually how much cycles we are considering for doing PCR ?

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