I am currently conducting research on RSV, and I am eager to know if the number of freeze-thaw cycles affects the titer after amplifying the RSV virus using HEp2 cells. Multiple freeze-thaw cycles may cause cell rupture and release of the virus, but repeated freezing and thawing of the virus can reduce the titer. Has anyone conducted relevant research and gained experience in this matter? Thank you very much.
Hello Cheng Zekai
Some viruses are quite fragile because of the presence of lipid membranes. Therefore, it is essential to know how to store and use them. The titer may drop 2-3-fold (or more) with each freeze-thaw cycle. So, it is best to avoid freeze and thaw multiple times. However, there are certain other viruses for instance, AAV which is stable than many viruses or proteins and can be frozen and thawed several times with minimal loss of activity, but it would be best to avoid multiple freeze thaw cycles.
You may want to refer to the article attached below for more information.
https://rupress.org/jem/article-pdf/100/1/89/1078266/89.pdf
Best.
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