How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

How to check normal distribution in a cell viability test?

To choose whether a parametric or a non-parametric test should be used to compare data on viability that emerge from an assay such as MTT, one should establish if the data are normally...

02 February 2021 1,733 4 View

How to create a graphic with two curve ??

Hello, i want to do a graphic with two curve to compare a toxicity of two metals in spss but i don't know how to do this. Can anyone help

01 February 2021 9,371 7 View

For lc50 study on fish against bacteria, the test performed referring to OD reading at 625nm. Is it acceptable method?

I would like to clarify regarding a method used to perform LC50 to infect fish by a particular bacteria strain. I prepared an overnight bacteria culture, collect the pellet from overnight culture...

05 December 2020 7,220 1 View

I want to use for my research 100 g of wister rats. what is the deferent with 200 gram Rats in biochemical Parameter?

the most of researches use Rat weighing 200g , is it ok to use rats weighing 100g ? and will it be the same LD50 of heavy metals ( I'm using Arsenic ) and what is the biochemical parameters could...

23 October 2020 2,809 3 View

Hi everyone. I try to determine LC50 of heavy metals in fish. Please how can I determine the concentration of heavy metals?

Is there any protocol for that?

10 October 2020 6,398 6 View

Which method is the most suitable for determination of LD50 of a plant extract?

As we have different methods for the determination of LD50 of a plant extract, such as OECD (up and down method), Lorke method, probit method, etc., so, which one among them is most suitable that...

25 September 2020 4,460 2 View

How to Determine LD50 of a test compound by Lorke's Method if your mice (mus musculus) dies at the lowest concetration i.e. 10mg/kg?

I have used Lorke's method of determining LD50 of my test drug. The lowest dose according to this method is 10mg/kg and my mice died at this dose within the 30 minutes of IP injection. Does that...

22 September 2020 9,959 3 View

What percentage of LD50 will be used for dozing experimental animals after finding LD50 using OECD method?

Plants extracts for doze albino rats for antidiabetic activity. LD50 was found using OECD standard method. What percentage of LD50 will be used or is it the doze used that will be used for further...

31 July 2020 6,313 3 View

What are the basis for determining assessment factors during chemical risk analysis?

I have EC50 and LC50 values and wanted to calculate PEC for different species in water. I have currently considered acute risk with an assessment factor of 100 based on a previous similar...

12 July 2020 4,562 2 View