Why do we wash azolectin with acetone?

I want to prepare liposomes made of azolectin (sometimes spelled as asolectin). I start with the azolectin powder, and many protocols say that I should wash it with acetone (dissolve, centrifuge,...

04 February 2021 2,738 3 View

How to solve 1-D Wave equation?

Good day, In standard PDE literature, there are a lot of solution approaches for the 1-D wave equation initial-boundary value problem. One way is with separation of variables. Below I describe...

25 January 2021 4,876 3 View

Journal Quartiles for Research Evaluation?

Dear all, Could you please tell me the countries where the journal quartiles SJR/JCR are used for the state system for research evaluation (except for Russia)? Best, Dmitry

21 June 2020 5,635 7 View

Could you please suggest an XRD data viewer?

Hi. I'd appreciate if someone could suggest a powder XRD (NPD, PDF as an option) data viewer which: (1) Is free of charge (2) Is easy to operate, i.e. can be run with a double-click, multiple...

06 June 2020 6,493 2 View

Could my SP2/0-Ag14 be cross-contaminated?

Hello! Currently I propagating my SP2/0 for hybridoma. It's a first time when I working with this line. According to ATCC this line must grow in suspension, but when I'm doing my daily observation...

20 March 2020 5,191 2 View

How we can painting with powder by electrostatic method without Faraday effect?

Good day, dear colleagues I have some question for You I'm working on the powder painting of details with difficult geometry. The tribostatic method has a good result about this I try to use...

25 February 2020 2,190 3 View

Is there any sources for 661-W cell line?

Hello! For my current project I need cell line 661-W - this is a mouse ganglion precursor-like cells. I had checked many supplies (ATCC, Sigma Aldrich etc) - but none offers this line. I even...

03 February 2020 1,011 2 View

Manuscripts for the 4th volume of the "Biodiversity, biogeography and nature conservation in Wallacea and New Guinea"?

26 November 2019 1,452 4 View

Where to go to the conference?

20 October 2019 6,938 3 View

Can I leave slides in Ventana overnight after finishing of staining program?

Hello everyone! On my previous job I worked with Ventana Benchmark Ultra and performed various IHC stainigs on this device. As far as I can remember, engineer said, that after finishing of...

21 February 2019 3,193 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View