I'm getting "Fatal error: Residue 'UNK' not found in residue topology database." how to rectify?

I've a new ligand and named it as 'UNK' in pdb file. While generating the ligand topology through GROMACS by following its protein-ligand tutorial. I'm getting this fatal error: " Residue 'UNK'...

01 January 2020 5,498 10 View

What should be the Protein concentration for assays?

for CAT activity, is there is any threshold regarding the concentration of protein in the enzyme has to be taken into consideration before doing the experiment.

07 March 2019 3,109 5 View

Why is in situ hybridisation preferred over immunohistochemistry in paraffin embedded mouse embryo sections?

I am trying to characterize early embryos and most literature I have read seems to prefer in situ hybridization over immunohistochemistry to study expression profiles of different proteins. Does...

13 November 2018 8,911 1 View

How can we perform the urease and nitrate tests for an alkalibacterium that grows only above pH 8?

The protocols available are optimised at ph 6.8 to 7.

07 September 2017 8,796 2 View

How to calculate Molarity ?

02 April 2017 7,315 8 View

Precipitation of DNA ?

For the precipitation of DNA during extraction protocol, which is preferred, among isopropanol and isoamylalcohol and what is the underlying reason to select it ?

01 January 1970 5,072 3 View

Preparation of Riboflavin solution for SOD activity

During the in-gel activity staining of SOD, Riboflavin is used in the concentration of 28-30micromolar. As per the literature/protocols, the preparation of the riboflavin solution is in 50mM...

01 January 1970 8,317 2 View

ROS assay quantification

I have done a ROS assay using DCFDA (Sigma #D688) in a lung cancer cell line by using a microplate reader at Ex/EM 480/530. I am looking to get the quantification of the data, is there any formula...

01 January 1970 3,567 3 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does any one can help me choose a reasonable basis set for intermediate metal ions interactions with guanine (i.e Fe2+, Mn2+, Cu2+ and exc.)?

Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...

01 March 2021 6,187 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

Should we reduce the standard unit of length to 0.31951540592 meter ?

The reasoning behind this question is to align the ratios of the proton potential in energy per charge to the speed of light in distance per time. As these two dimensions appear to be related,...

27 February 2021 947 7 View

What could be reason for getting such type of graph for complex impedance in case of polymer blend films?

Its effect appears in real part of ac conductivity also. Can anyone suggest any reason behind it?

26 February 2021 3,487 3 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View