I am trying to ligate a 3Kb band isolated insert to a 12.2 kb vector and I am having issues with transformations. I hardly see colonies on the plates and even if they are formed it is very less. Any suggestions to troubleshoot my issue?
Hello everyone, Regarding the SDH assay calculation on a Microsoft Excel sheet, I need assistance. Using the UV spectrophotmetric approach, I conducted an in-vitro SDH experiment in the A549 cell...
21 May 2024 4,985 0 View
how to extract collagen from bones specimen?
27 April 2024 6,307 0 View
Can coulombic efficiency be greater than 100 for galvanostatic charge-discharge if the discharging time is greater than the charging time at constant currents? Is there something I'm doing wrong?
18 January 2024 244 0 View
Structure of NiCr LDH DFT and input file
07 November 2023 5,493 0 View
I am working on an Intrusion Detection System model. For its deployment i want to extract the network connection attributes of an Internet Connection in real time. Can you suggest me some sniffer...
23 October 2023 995 4 View
I am currently working to create an AI/ML model for non-IoC(Indicators of Compromise) based Intrusion Detection System. I am having trouble in finding out where exactly can this model be deployed....
15 October 2023 203 2 View
I was given a theme 'Satellite Oceanography', that is we need to apply Machine Learning Techniques to solve the problems that occur in Oceans that can be solved using Satellite Oceanography. I...
15 October 2023 6,683 0 View
This question addresses the practical considerations for optimizing CRISPR-mediated knock-in experiments in mammalian cells. It explores aspects like donor DNA design, homology arm length,...
13 June 2023 7,669 0 View
How to calculate parameter ∆c which represents the axial deformation at the expected buckling load for braces as per ASCE 41.
02 November 2022 2,850 0 View
As per ASCE 7-16, I am using following load combination. Where Emh =Ω0*Qe. where Qe is effects of horizontal seismic forces from V. But, I am not understanding, how to calculate Qe 1.2D + Ev +...
27 July 2022 2,686 0 View
I tried four trials of the same Copper Phosphides sample in Alkaline medium ( 0.5M KOH) with Hg/HgO reference electrode and Pt as counter electrode. I used 0.001 V/s scan rate for first three...
10 August 2024 3,629 1 View
We have observed that tube to tube sheet joint leaked in our boiler and needs to overcome same by knowing the root cause.
08 August 2024 3,161 0 View
I have face this problem anyone help me how to solve this issue ?which is below Fatal error: There are inconsistent shifts over periodic boundaries in a molecule type consisting of 78 atoms. The...
07 August 2024 2,598 1 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
Dear Researchers I need to know, how to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field data in Tecplot? Thank You
02 August 2024 3,615 1 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...
29 July 2024 950 4 View
Hey All! I am wondering what might be wrong with my band structure. I did the calculations using VASP and plotted the results using Origin. Although I have tried changing various input...
25 July 2024 2,920 11 View
It is being seen that Editors of some reputed Journals are putting on hold the manuscripts and not updating review states even passing more than six months from submission. Researchers are...
24 July 2024 8,596 2 View