I am seeking clarification on the differences between the two commercial baculovirus systems for insect expression: Bac-to-Bac system (Invitrogen)and the MultiBac system (from by Imre Berger and colleagues) for insect expression.
It has come to my attention that specific plasmids, such as pACEBac1/pFBDM for MultiBac and pFastBac/pFastBacDual for Bac-to-Bac, are associated exclusively with their respective systems. Would someone be able to confirm whether these plasmids are indeed non-interchangeable between the two systems and, if so, an explanation for this distinction. The plasmids look similar, so is there a difference in the mechanism?
Only main difference between the two systems is that the MultiBac system allows for construct assembly using CRE/Lox recombination before Tn7 transposition. The plasmids should be interchangeable.
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