Established cell cultures that have the potential to propagate indefinitely. | Contact experts in Cell Line to get answers
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Questions related to Cell Line
Hi I have stem cells encapsulated in sodium alginate and I want to stain them for Fluorescence microscopy. What is the best concentration of the DAPI and PI ? Do you have any protocol for that?
27 July 2023 3,072 3 View
I have read that I can use RIPA buffer for EV membrane disruption. Does this have to be followed by ultra centrifugation? Does anyone have a protocol for this? Thanks.
27 July 2023 2,461 5 View
I am looking for a PhD Position in Microbiology, Virology, and Molecular Biology.
27 July 2023 7,529 1 View
What are the potential indicators of microplastics pollution?
26 July 2023 5,019 2 View
Hi everyone. I am purifying a recombinant protein using affinity chromatography and SEC. Since this protein has some free cysteines, to prevent them from forming improper disulphide bonds leading...
26 July 2023 5,886 7 View
The particle-to-protein ratio has been widely used to determine the purity of exosomes. Usually, NTA is used to measure the particle concentration (particles/mL) of the exosomes while microBCA...
26 July 2023 358 0 View
Is the pyramid of biomass always upright in an aquatic ecosystem and how is a pyramid of biomass connected to an ecosystem?
26 July 2023 9,868 3 View
Hi, I am going to use RIPA buffer to do protein extraction. However, I am wondering whether I need to heat the samples (eg. 95°C, 5mins) to get better solubility? (The downtream work is western blot)
26 July 2023 3,795 2 View
I have been treated PC12 cells with 100ng/ml NGF for 10 days, and lysed them using RIPA buffer for western blot. I got no bands for primed cells, but for wild type cells. Does anyone know why?!
25 July 2023 2,476 3 View
Need to add a gfp tag as well.
25 July 2023 1,523 5 View
Hi! We had a cell culture contamination for several weeks in different cell lines (HepG2, MCF-7, MDA). We have discarded all reagents, clean the waterbath and all the surfaces and started all over...
25 July 2023 5,526 4 View
Some genes that resulted from my differential gene expression analysis don't have corresponding ENTREZ ID and I want to perform KEGG pathway analysis but most tutorials analyze data with no...
25 July 2023 3,956 2 View
Hello all, We cultured MSCs on calcium phosphate discs for 3 days and 7 days. We are seeing strange crystal-like precipitates or something of the sort (images attached). They are found wherever...
25 July 2023 3,871 4 View
I wish to protect the protein from proteinase enzymes when using (yeast) to purify human papillomavirus protein, Which enzyme inhibitor is alternative to PMSF? which to be stable in high...
25 July 2023 7,254 0 View
So, i would like to know what concentration of Tris HCl i have to prepare in order to use it in RNA isolation protocol? Additionally, i would like to know why to use this buffer, is it only for pH...
25 July 2023 5,471 3 View
I would like to add BG-azide to cells for SNAP tag pulldown however I don't know whether it is going to be cell permeable. My instinct is to day yes it will be but neither me nor the supplier know...
24 July 2023 462 5 View
I have seen research papers using concentration much higher than 18uM and no one has reported that they encountered similar problem. I have made a 10mM stock (in DMSO) and dilute it with DMEM to...
24 July 2023 9,578 2 View
Hi everyone, I'm using a dual glo luciferase assay by promega to assess Wnt activity in my Wnt conditioned media. The setup in my protocol requires to use -a negative control (NEG, non responsive...
24 July 2023 3,463 4 View
I have performed anti-Oct-4 staining with A488 on a colony of hiPSCs. In ZEN, I have applied an intensity channel (rainbow) to the image. As you can see, the highest intensity is located in the...
24 July 2023 6,795 1 View
Dear all, For my last two experiments, my supposedly endothelial cells (differentiated from bone marrow-derived mesenchymal stem cells, at passage ~35) have detached from Transwell inserts 1-2...
24 July 2023 4,485 0 View
Is it normal for some journals to make millions of dollars without paying their editors or reviewers?
22 July 2023 4,765 4 View
In research to investigate the expression of genes in breast cancer, which of the housekeeping genes is the best gene for QRT-PCR؟
22 July 2023 3,152 1 View
Topics related to materials, polymers, composites materials, cement materials, sustainability and so on.
21 July 2023 7,745 4 View
Is anyone familiar with the specifications of Silica (brand, catalogue number) is as good as silica packed in commercially available silica columns for DNA purification?
20 July 2023 5,082 0 View