Established cell cultures that have the potential to propagate indefinitely. | Contact experts in Cell Line to get answers
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In the literature, there are two common PI3K antibodies, 85kDa and 110kDa subtypes (such as p85α and p110γ), which are known to correspond to different subunits (p85 is the regulatory subunit and...
24 June 2025 5,003 1 View
Hello, We conducted a lab where DNA was cut with the restriction enzyme EcoRV. All samples were treated under the same conditions (same EcoRV enzyme, buffer, 37°C for 2 hours). However, well 8...
21 June 2025 5,841 4 View
I am working on modeling an electric heating film with power 𝑃 integrated into a floor using COMSOL Multiphysics (2D model). I created a thin domain of 2 mm thickness to represent the electrical...
20 June 2025 521 2 View
In recent years, significant ozone concentrations have been noted in the interior of a number of states in the Arabian peninsula, including Saudi Arabia and the United Arab Emirates: S. Osipov et...
14 June 2025 8,841 2 View
The Middle East is on a hot plate. Do you think Iran will respond to the strike launched by Israel?And what is the expected impact of these skirmishes on the economies of the Middle East and...
13 June 2025 9,216 88 View
We are currently generating NK cells from iPSC-derived embryonic bodies (EB). After 1 Week HPC (CD34+) Differentiation in APEL2 medium (+Y27632, SCF, VEGF. BMP4) followed by 3 Weeks in NK...
11 June 2025 7,759 1 View
The software being free is prioritized
25 May 2025 111 3 View
Hello everyone, In the next month I am going to field to collect data for my PhD. I want to measure the amount of pollen carried by pollinators and the amount of pollen deposited on stigmas of...
23 May 2025 4,424 0 View
I am trying to replicate the results of a research article titled 'Improved empirical model for the Jerusalem Cross' by using the same technique of the equivalent circuit model mentioned in the...
13 May 2025 8,654 0 View
The simulation results of abaqus' 0-thickness cohesive element show random lines.
05 May 2025 369 1 View
"Should AI-powered tools replace traditional classroom instruction in English Language Teaching?" Why?
03 May 2025 5,712 5 View
Hello, I am trying to exfoliate monolayer flakes of InSe and ReS2 for my device. Using scotch tape directly on the Si chips hasn't been very helpful, as it leaves a lot of debris and compacted...
29 April 2025 2,646 2 View
I'd like to ask if there are any existing studies that give a visual reference for reading the otolith increments of Selar crumenophthalmus in a compound microscope, to be used for age analysis?...
29 April 2025 7,342 0 View
How do biological contaminants from plant extracts or microbial cultures affect the purity and functionality of the final nanoparticles?
28 April 2025 9,231 5 View
Hello, I am looking for languages which have not (yet) adopted CAN YOU as a way of making requests - as in "Can you pass me the salt". If you are a speaker of such a language, could you let me...
26 April 2025 5,319 2 View
I am currently conducting an experiment in which I aim to induce diabetic ketoacidosis (DKA) in rats in order to measure isopropanol levels. While I have been able to successfully induce diabetes...
25 April 2025 3,240 2 View
I am looking for a method to remove residual EDTA from human plasma samples before the downstream use in cell culture. The goal is to minimize potential barrier disruption caused by EDTA remaining...
22 April 2025 5,507 1 View
Two book chapters that will be read for years to come as sources of food for thoughts Citation: Muñoz, Lucio, 2025. Dwarf Green Markets vs. Green Markets: Evaluating their Role in Sustainable...
22 April 2025 6,046 2 View
First of all, I am unfamiliar with using monographs for critiquing research. In this case, I am tasked with critiquing a qualitative research project in linguistics, discussed in monograph...
18 April 2025 2,095 2 View
what would be the basis for sampling from big number of students' population?
16 April 2025 1,303 3 View
I performed the total flavonoid content assay using sodium nitrite, aluminum chloride, and sodium hydroxide solutions. However, my extract has a strong inherent color (dark yellow and dark red),...
09 April 2025 733 1 View
Can anyone share their established lab protocol that works well for mouse brain tissues and also do we have to isolate genomic dna from chromatin to check the sonication fragments or we can...
08 April 2025 9,437 0 View
Putative AMPs received in lyophilised form, would like advice on which solvents to use that are suitable for in vitro antimicrobial activity assays. The majority of aa sequences of peptides have a...
07 April 2025 7,796 1 View
I've been encountering several errors when working on my relative-qPCR. My project uses two different assays to assess telomere length against a single-copy gene using SYBR as the dye of...
03 April 2025 9,954 0 View