Established cell cultures that have the potential to propagate indefinitely. | Contact experts in Cell Line to get answers
2,570 views 8,692 posts
Questions related to Cell Line
I labeled exosomes with DiR and administered them to the mice (o.g.). After 24 hours, I performed perfusion and harvested the organs. I could observe signal from the liver using IVIS system. I...
15 November 2023 4,967 0 View
I am currently performing co-culture experiments with murine CD8 T cells isolated from mouse spleens and with skin cancer cells (PDV) in a 24-well plate. I first isolate the CD8 T cells from...
15 November 2023 5,034 2 View
I'm looking for a Crhr2 antibody suitable for immunofluorescence in mouse brain slices. I've tried ab236982 from Abcam, but it didn't work well for me. Does anyone have any other...
15 November 2023 9,391 0 View
Hello everyone, please what is the optimum Plasmid concentration after extraction with the mini prep method, Please your kind response will be helpful. Thanks !
13 November 2023 2,262 3 View
I'm trying to work on a new topic with a new challenging subject
13 November 2023 4,088 0 View
What are the amino acid concentrations in skin interstitial fluid? especially, Trp (Tryptophan), Tyr (Tyrosine), Leu (Leucine), Ile (Isoleucine), and Val (Valine) thanks.
12 November 2023 925 0 View
It's important to remember that while there may be conflicts and divisions among people based on various factors such as race, nationality, religion, or ideology, it is essential to recognize and...
12 November 2023 7,508 8 View
Lots of studies in adults and children, mainly done in HICs, are advocating antibiotics only (conservative) management of acute non-perforated appendicitis. In LMICs, with poor access to...
11 November 2023 3,521 1 View
I prepared PBMCs,s from human blood and differentiated them as immature dendritic cells in this process, monocytes adhere to the plates. What could be the reason for this phenotype?
10 November 2023 8,237 2 View
Hi there. Does anyone know if microalgae (chlorophyta in particular) can use EPS (or polysaccharides) as source of energy? Can you reccomend me any paper where polysaccharides have been used as...
10 November 2023 6,214 2 View
Hello everyone, I was wondering if anyone could tell me about the stability time limit for dU-ssDNA at -20C. I am currently developing a synthetic phage display library, and I have recently...
10 November 2023 3,285 0 View
I have two datasets (both with experimental group and control group) which measure the same construct with two different forms due to age differences. All groups completed the measure at a pretest...
09 November 2023 6,639 1 View
How to use a confocal microscope to photograph a material hydrogel microneedle sample of about 5mm*5mm*1μm (height) size, and how to place it? What are the precautions? Example has shown in the...
09 November 2023 4,219 0 View
I have just started machine learning programming in MATLAB and I need dataset in algorithms to apply on.
08 November 2023 4,578 2 View
I'm searching for real examples of increasing of fusion efficiency and the yield of functional cells in mice hybridoma technology. Did you use magnet separation or FACS, or was there no critical...
07 November 2023 4,255 0 View
adverse childhood experiences may include physical and emotional abuse. so how it could be a cause of chronic diseases?
06 November 2023 4,473 3 View
I am doing my research on psychological resilience in adolescents, juvenile and non-juvenile, and for this purpose, I am searching for an resilience scale for data collection. please suggest to me...
06 November 2023 1,935 2 View
Dear colleagues Good day to you all. One of the most famous debates in Igneous Petrology is the relation between the diamondiferous rocks (i.e. lamprophyres, lamproites, orangeites and...
06 November 2023 779 0 View
In the progress of pulmonary fibrosis, fibroblasts proliferate， or differentiation to myofibroblasts , what's the difference of these terms?
06 November 2023 9,314 1 View
Hi , I have been noticing increased differentiation in my mouse embryonic stem cell culture. We culture cells in both 15%FBS.KODMEM media as well as the the 2i /N2B27 media. For the serum...
06 November 2023 9,668 0 View
I have been working to troubleshoot my Iba1 stain for microglia for the past few months with no avail. Looking to stain Iba1+ microglia in fresh frozen, OCT-embedded rhesus macaque brain, slices...
06 November 2023 1,305 1 View
I would like to ask if there are any studies focusing on cognitive developmental issues or mental health issues in the primary immune patient population. If so, are there teams working on this...
05 November 2023 2,098 2 View
Even thought classes are persona, not persona but many teacherscassignnpersinas, with psychological traits, to student classes, especially in difficult ones. They believe this gives them a...
03 November 2023 7,109 3 View
I ran "FUNGuild" for fungal OTUs functional annotation but the column of "TrophicMode "(in taxonomy.guilds file) is showing "na" values for all the fungal OTUs. Is this normal and what can be the...
03 November 2023 4,683 0 View