Established cell cultures that have the potential to propagate indefinitely. | Contact experts in Cell Line to get answers
2,268 views 6,669 posts
Questions related to Cell Line
Dear all, I was wondering if anybody has any tips regarding the transfection of human melanoma cell lines (source ATCC) using either the Neon or Nucleofactor electroporation systems. Looking...
14 May 2023 3,260 1 View
As title, my sample is kind of plant-based protein(pI=4.5) and its hydrolysates and glycated protein. I treat my sample with CACO-2 cells in MTT test, but I found that my sample is ph-sensitive,...
14 May 2023 4,588 2 View
In the picture you can see a colony of adhered cells and some loosened cells. Also, you can see small clear small dots. Those are the vessicle-like structures im not sure what they are. To give...
13 May 2023 269 3 View
Let's assume that when the cell viability experiment was conducted using animal cells other than HeLa cells, the absorbance of the negative control (cells not treated with Doxorubicin) showed...
13 May 2023 6,091 1 View
Hi, I would be thankful if you could help me with some issue in IF staining. I've fixed some rat brain tissues in PFA (4%) then preserved in sucrose-PBS (30%) for 7-8 months. Does long storage in...
13 May 2023 828 5 View
If I want to perform an assay with a specific cell line that has been cryopreserved for over 5 years will it affect the results of my study?
12 May 2023 5,274 3 View
Does FBS contribute to prion's proliferation
12 May 2023 3,554 2 View
--
12 May 2023 4,977 0 View
We use E3019-3.5KU. We store at -20C, but after 3-4 times of opening, the esterase in the bottle loses its activity. We aim to use it in PBS buffer 7.4. Have anyone stored esterase in PBS buffer...
11 May 2023 7,373 2 View
Hello, I am currently confused with a few following steps in BSA denaturation inhibition assay. 1. Some protocols stated that the buffer was added along with BSA and tested solution before...
11 May 2023 3,310 3 View
I am planning to generate a KO cell line, using RNPs I am using a paired gRNA system to induce a deletion of a segment instead of just indels. Generally the RNPs are formed using a 1:1 molar ratio...
10 May 2023 8,584 4 View
It's not a secret that while truly pure imidazole does not have absorbance above 260 nm, typical imidazole products on the market (such as ACS, "Ultrapure", etc grades) do have absorbance of about...
10 May 2023 4,840 3 View
Dear Colleagues and friends, as Co-Chairman, I would like to invite you to join us and present your work at the VII INTERNATIONAL CANCER IMMUNOTHERAPY CONFERENCE (CICON23) – TRANSLATING SCIENCE...
10 May 2023 4,084 2 View
What are the basic statistics that we can use to calculate the accuracy of glucometers?
09 May 2023 5,696 1 View
Could you kindly provide me with references to studies that evaluate the relative impacts of geographical distance and directional distance on active transportation mode choice, such as walking...
09 May 2023 6,728 5 View
I am doing multiplex immunofluorescence assay on FFPE section. I used CD3 to identify T cells and observed that some of T cells stained with CD3 showed nuclear staining instead of membranous. I am...
09 May 2023 3,898 4 View
In some cases the -10 of the promoter is known, while in others it is not. In that case how do we predict the transcription start site (TSS)? I have tried using the popular tools available. They...
09 May 2023 3,395 6 View
Aslamo alikom/Hello I'm planning to do MTT experiment this week but unfortunately, we ran out of phenol-free media (PFM), which is added to MTT reagent for nearly 4 hrs. As it'll take awhile to...
07 May 2023 439 3 View
My 7years old German Shephard can not walk on his back legs suddenly. There is no fracture of legs. But my dog cannot able to walk. Please help.
05 May 2023 9,913 3 View
Q1: Are the GSCM, circular economy and I5.0 is operational practices or managerial practices in an industry? Q2: Does ISO and OSHA guidelines fall under environmental conservation...
05 May 2023 1,492 1 View
Any summer program to have hands on experience in patch clamp recordings ? also, any online theoretical course that you find beneficial ?
05 May 2023 8,347 5 View
Hello, I am trying to count Red Blood Cells in Invitrogen's Countess II FL equipment. Which parameters do I need to set up? Many thanks!
05 May 2023 5,260 0 View
I would like to reusethe ultra-low attachment plates from Corning for spheroid assay experiments. I had an agar and a collagen layer in the plate. Does anyone try to remove the agar/collagen...
04 May 2023 4,765 0 View
Thank you.
04 May 2023 1,196 2 View