We are currently generating NK cells from iPSC-derived embryonic bodies (EB).
After 1 Week HPC (CD34+) Differentiation in APEL2 medium (+Y27632, SCF, VEGF. BMP4) followed by 3 Weeks in NK Differentiation medium (+SCF, IL-7, IL-15, IL-3, Flt3L) NK cells are generated.
However, during this process a large bubbles are generated which are visible via eye. These increase during this process by size and have a ball-like transparent structure.
Therefore, we are investigating if these are cystic embryonic bodies or other necotric cell debris (see picture, left side).
Many thanks for any recommendations.
Kindest regards
Have you take a sample and put it under microscope for histopathology interpretation?
If it was cystic, there should be structured cells formation in bubble layer membrane. If not it should necrotic debris
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