Established cell cultures that have the potential to propagate indefinitely. | Contact experts in Cell Line to get answers
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Questions related to Cell Line
I found that an organ contains an enzyme that can deacetylate N-acetylserotonin, but I cannot identify it. Does anyone have suggestions for identifying the enzyme?
12 June 2023 1,025 5 View
I am doing the PC12-C6 co-culture system and would like to double stain the cells to observe the cell to cell interactions. I first used the mouse FITC-conjugated anti-beta III tubulin antibody,...
12 June 2023 2,372 7 View
How can optical design and system layout be optimized to increase the concentration of solar energy onto the heat transfer fluid and improve the overall efficiency of a CSP power plant?
11 June 2023 7,111 1 View
I want to know the Relative abundance of Antimicrobial Resistance Genes by qPCR (I Will compare the abundance of ARGs and the abundance of bacteria in my sample). I Will use ARGs primers and 16s...
11 June 2023 4,216 1 View
How do the latitude and the tilt in the axis of the rotation of the Earth affect the amount of radiation received at the Earth surface?
11 June 2023 9,287 1 View
What could only be called "attention' sometimes precedes perception. Realizing that, and you are on your way to becoming me (you're welcome, in advance). I am not just a "cog in the machine"...
10 June 2023 6,790 6 View
Objective: I'm launching a series of new experiments and will need some answers and advice. My questions: 1- do you know of any studies that looked at the effect of adding pro-inflammatory...
10 June 2023 5,319 0 View
It seems that right now, i can't edit the details of an article i recently added to researchgate. The abstract contains a few typos, and the publication date is wrong. I tried to fix the issues...
09 June 2023 7,478 1 View
I am hoping someone here can give me a little help with my NMJ staining. I am staining mouse so keys and tibialis anterior muscle. The stain works well but even though I have teased the muscles...
09 June 2023 8,342 1 View
Which journals with high impact factors can research on laser-induced breakdown spectroscopy be submitted to?
09 June 2023 9,993 5 View
The set of optimal solutions obtained in the form of Pareto front includes all equally good trade-off solutions. But I was wondering, whether these solutions are global optima or local optima or...
08 June 2023 3,298 3 View
Which part of a plant cell is responsible for controlling activities of other parts of cell and organelle controls daily activities & reproduction of the cell?
08 June 2023 9,975 1 View
Good day everyone, Please I will be performing CRISPR cas9, I don't know how to go about this, I will appreciate it if anyone can assist me, I don't mind having a video to guide me step by step....
07 June 2023 2,876 3 View
We are working with SH-SY5Y for many years but did not face problems like this. Cells morphology have changed and the freeze that we are keeping at -80 or even in liquid nitrogen are not attaching...
07 June 2023 2,813 5 View
In addition to the patch clamp technique, what other techniques can be used to evaluate the function of neurons and neurites in a laboratory environment? we don't want to implant our scaffold...
07 June 2023 2,597 0 View
my recombinant vector contain thioredoxin tag and my elution buffer contain imidazole, NaCl and NaH2PO4. so how can i replace my elution buffer with tris-Cl buffer before enteokinase treatment
07 June 2023 4,543 4 View
Hello, I'm working with iPSCs and culturing them on feeder-free conditions using Matrigel. I'm using the Corning Matrigel for Organoid Culture as that was the only Matrigel that was being shipped...
06 June 2023 4,788 2 View
What should be the ideal resection and anastomosis?
06 June 2023 6,713 4 View
In fact the surprising geometric rule: two 3D bodies of different shapes cannot have the same volume to area ratio unless both have exactly the same volume and same area, is a physical rule...
06 June 2023 7,292 2 View
Hi all, I have been trying let B16F10 cells to produce and secrete Melanin upon a-MSH induction. Cells were induced with 50nM, 100nM and 500nM a-MSH in complete DMEM including 0.5mM L-tyrosine...
06 June 2023 2,903 0 View
Hello I am searching for a suitable 16srRNA primer for identifying bacteria in the genus of pseudomonas. I have found many sequences and I dont know which is the best. Can anyone help me what...
06 June 2023 5,899 4 View
Can someone send the 24th edition of standard methods for the analysis of water and wastewater (APHA) ?
06 June 2023 1,567 0 View
I encountered the problem of no signal detected from the flow analysis of my THP-1 derived dendritic cells (DCs). I have observed the morphology of my cells resembling that of DCs after...
06 June 2023 2,546 1 View
I want to use a RALA peptide vector, and I have found that some studies centrifuge the plasmid/RALA complexes and resuspend the pellet before use, while others seem not to do so. It seems like the...
06 June 2023 2,554 2 View