Established cell cultures that have the potential to propagate indefinitely. | Contact experts in Cell Line to get answers
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Questions related to Cell Line
I usually find that till the centrifugation step I can see the pellet, but after that even when I'm being really careful, I lose major amount of cells. Is there any way I can save them from...
26 December 2023 2,998 1 View
I have cloned my genes in a CMV vector and transfected them in HEK293 cells. After harvesting the cells, the expression level at the transcriptional level is increased compared to the control...
24 December 2023 2,445 10 View
Hi, I just have been in fusion cloning. I want to insert my GOI in pMIG II vector. So, I amplified my GOI using PCR with 15bp overhang (PFU used), and linearized the pMIG II vector with EcoRI...
24 December 2023 7,023 3 View
I am performing bi-layer well diffusion antibacterial assay of plant extracts. At the bottom of the plate, I pour hard agar media ( nutrient broth + 1.5% agar). Then, I mix bacteria from broth...
23 December 2023 4,940 1 View
How to interpret saturation index (SI) values for compounds in aqueous media if SI is 1. large negative 2. small negative 2. small positive (0-1), and 3. large positive (>1)? SI tells us about...
23 December 2023 3,420 0 View
For the past few weeks, I've been attempting to passage U-87-MG cells in our lab. However, after thawing, instead of reaching 80% confluency within 24 hours, they're taking 3-4 days to achieve the...
22 December 2023 450 2 View
#Stress management #Academics Performance
22 December 2023 4,597 2 View
I have a question, I have noticed that when I extract dna, in the last step when I resuspend in either 50 microliters of TE buffer or water, the total precipitated dna doesn`t solubilizes. I haved...
22 December 2023 109 1 View
What should be a valid explanation for including only male mice in depression studies (Earlier estrous cycle phase hormonal changes was one of the reason for excludinng female mice, which is not...
21 December 2023 2,227 2 View
I am trying to see a quencher (BHQ)tagged synthetic DNA oligonucleotide on an agarose gel...I used EtBr for the same...I was able to visualize the strands that I have (not tagged by BHQ), but I...
21 December 2023 2,084 0 View
I would like to ask a question about constructing stable cell lines. If someone has the whole genome sequencing results of their overexpression stable cell line that would be really helpful. That...
20 December 2023 9,384 2 View
For İn-vitro studies İ am trying to use resvertraol as an antioxident to check for its neurprotective effect and apparently there are two types so İ was wondeing which one should İ use
19 December 2023 8,396 1 View
Hi all, I recently started working with flow cytometry with no prior knowledge. I have been trying to stain cells to practice flow cytometry techniques, such as panel setup, gating, and data...
18 December 2023 9,324 2 View
Hi everyone, im using this established protocol: Establishment and Culture of Human Intestinal Organoids Deri... I already optimized everything, written under "troubleshooting" in this protocol....
16 December 2023 4,959 4 View
I'm zoologist, but I would acknowledge very much if someone could supply me examples of known (references) complex species in plants, algae or fungi. Thanks a lot. Juan Lucas Cervera.
16 December 2023 5,117 12 View
I think SPSS made my computer slow. Be careful when run in your computer.
16 December 2023 6,743 3 View
Hello, When you want to investigate exacerbations (outcome of interest) in asthma, you can measure: 1) the incidence rate in the last year (i.e. mean numbers of exacerbations in the last year in...
15 December 2023 9,767 1 View
Hello! We are generating conditional knockout mice using CX3CR1 Cre for a particular gene. During this process, we are performing tail cuts for genomic extraction and genotyping. We've designed...
15 December 2023 4,623 1 View
After opening up the mice, sometimes I notice white streaks on the liver as shown in the picture. The mice are usually 6-8 weeks old, fed regular chow and water. No treatment was applied and we...
14 December 2023 3,559 0 View
how to take sample from unknown population? which research design and sampling method is recommended?
14 December 2023 4,256 3 View
I have an (online questionnaire) in Radiation Oncology field need to be validated by radiation oncologists, any one can help?
14 December 2023 5,361 1 View
can I use the cox activity assay kit with fish liver lyophilization samples??
14 December 2023 8,863 2 View
Schiller and Carvey (2006) studied perceptual grouping of 4 x 4 clusters of stimuli (see Fig. 1) by having subjects view a collection of quartet stimuli (a cluster) composed of a central spot with...
13 December 2023 4,796 0 View
Hi Everyone, We are searching the method about the lowest dosage of DMSO as a non-toxic dosage for direct injection cryopreserving cell in animal/human body which keep the cell...
12 December 2023 2,153 5 View