Established cell cultures that have the potential to propagate indefinitely. | Contact experts in Cell Line to get answers
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Questions related to Cell Line
Can I culture HEK293T cells and MSCs together (co-culture)? What would be the best method? How can I extract RNA from HEK293T only to monitor gene expression from being co-cultured with MSCs...
20 June 2023 1,155 3 View
To measure and detect MicroRNAs, researchers face many challenges, Some of them are: 1- Small size 2- Similarity in sequence in the same family 3- A tiny amount in the sample
20 June 2023 6,028 3 View
Has anyone ever tried to culture Jurkat cells in serum free media (+/- additional growth factors)? I'm looking for a way to reduce the amount of glycoproteins/lipids for prolonged culture. Best...
20 June 2023 452 6 View
What is the difference in terms of content? Is Opti-MEM w/ FBS harmful to cells?
20 June 2023 3,484 3 View
Can I take a plasma sample from a sepmate tube to test for biochem or vitamins?
19 June 2023 6,259 1 View
In a patient with desminopathy (mutation Thr341Pro DES in the heterozygous state) with the progression of the disease, we note signs and symptoms that are also characteristic of botulism:...
19 June 2023 2,464 6 View
Does anyone knows how to normalize the data obtained for MTT assay result to 100%? Because when I count the %viability I get more than 100% and upon consulting my supervisor, she told me to...
19 June 2023 4,725 6 View
We tried to isolate plasmid by Alkali Lysis method. In the circled region we could not visualize so what may be probable reasons for that
18 June 2023 568 1 View
What is the Maldi-tof technique? Is it necessary to perform Maldi-tof technique after protein purification?
17 June 2023 9,549 4 View
Hi all, I am using proteomics to conduct a microbiome experiment, and one of the sample types I will collect is the mouse cecum. I see various techniques in the literature, some papers wash the...
17 June 2023 3,684 1 View
Using data from 11 wells on a 24-well MEA, I have collected repeated-measures data on the number of events (spikes) occurring +/- 1 second of a stimulation event. We ramped up the voltage from...
16 June 2023 6,975 0 View
The cell cycle of my MCF10a cells sometimes looks "compressed" with no clearly distinct phases. Other times I get a cell cycle that looks perfectly fine with clear phases and I can´t find a reason...
15 June 2023 1,481 2 View
I'm currently facing a challenge with c-fos staining, and I've made several attempts to resolve the issue. However, even after trying different antibodies, I consistently observe the same staining...
15 June 2023 9,598 1 View
Hello guys, We are having some issues with this qPCR. We run qPCR quite frequently, and we never seen a curve like that (I mean, if I can even consider this a curve at all) Looking at the...
15 June 2023 5,030 0 View
Hi! I have used an online program to produce siRNAs for a custom target. I seem to be getting some sort of off-target silencing with some of my siRNAs. I was wondering if anyone knows of a free...
15 June 2023 4,148 1 View
I have synthesized czts using hot injection method. how to prepare sample for HRTEM characterization?
15 June 2023 3,615 2 View
Since compensating on Kaluza is easier than Flowjo, I used it. But now I need to put data in Flowjo for analysis. Is there a better way than typing to import compensation matrix made in Kaluza to...
15 June 2023 5,633 3 View
Electro chemistry, Engines, Vehicles,
14 June 2023 9,654 3 View
1. A.baumannii specific phage.
14 June 2023 8,606 2 View
I have used my amicon filter one time for concentrating down the TEV protease. I want to re-use the device again in future for the same protein. How to store? Buffer and storage temperature?
13 June 2023 2,768 3 View
When performing pharmacological assays, can I prepare dose response curve of specific assay and keep in freezer to be used over few days? Or I should do it fresh every time.
13 June 2023 2,905 3 View
By pushing the boundaries of conventional thermodynamic cycles, exploring new materials, and embracing sustainable technologies, I, as a researcher, can aim to enhance the efficiency of energy...
12 June 2023 5,225 4 View
I culture human iPSC in monolayer for my research, kept in 6 well plates coated with either matrigel or laminin, and kept in either mtesr or iPSC brew (sometimes a 1:1 mixture). Previously they...
12 June 2023 5,440 1 View
The isolated T cells will be further activated and expanded in vitro for transduction (with chimeric antigen receptors). The ex vivo culturing will take about 2-3 weeks. I know that for isolating...
12 June 2023 1,348 1 View