Cell Line

Established cell cultures that have the potential to propagate indefinitely. | Contact experts in Cell Line to get answers

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Questions related to Cell Line

Can Tx -100 cleave GST from fusion protein?

Hi. Recently, as a mean to increase yield, I added 0.1% of Tx-100 into all of my IEX purification buffer including the sample injected. However, when analyzed on SDS -PAGE, I observed that there...

28 November 2023 2,283 3 View

Can Welch test be used with equal variances?

I have a setting of 4 groups of normally distributed data and unequal sample sizes. Alpha-level is set at 0,05 in all analyses. To see if there are any differences between the groups I've run...

28 November 2023 6,287 18 View

Which is the best practical manual for plant pathology(Fungi)??

I want plant Pathology practical manual related to fungi, like isolation, identification, different pathogenecity test etc. Can anyone please suggest it.

27 November 2023 4,288 1 View

Why geochemical analysis for major oxide is important in metamorphic rocks and magmatic rock?

https://www2.tulane.edu/~sanelson/eens212/triangular_plots_metamophic_petrology.htm

27 November 2023 5,845 1 View

Can someone help me find out the latest classification of bacterium Citrobacter spp.?

I find it difficult to find a references that shows the classification of Citrobacter spp. from ancient to modern and the historical events of its discovery.

27 November 2023 3,788 2 View

Transduction of Osteoblast with Retrovirus or lentivirus?

Dear colleagues, I tried to transduce mouse osteoblastic cells with retrovirus many times but I failed, do you have any recommendations? Thank you in advance.

26 November 2023 1,603 0 View

Which technique is more cost-effective and approved to evaluate the functionality of neurites? patch clamp or multi-electrode arrays (MEAs)?

I want to functionally evaluate scaffold-grown neurites in vitro and need a validated technique that provides results at a low cost and in a short time.

26 November 2023 4,349 1 View

For a lot of structures I'm not getting the value of open circuit voltage and field factor in scaps 1d which parameters need to change?

In SCAPS-1D I am trying different combinations of HTLs, ETLs, and absorption layers. But for a lot of structure, I'm not getting Voc and FF. I tried to change thickness, affiniity, bandgap, and V2...

25 November 2023 1,509 1 View

Estimated protein concentration in HUVECs?

Dear community, our group is working with HUVECs for the first time and we are lacking some experience... Does anyone know the protein yield of HUVECs? Do you have an estimation how many...

24 November 2023 513 1 View

Which data analysis is better correlation or regression for a thesis?

In my thesis I calculated two of this method correlation and regression. But I can't understand which is better for data analysis.

24 November 2023 8,659 4 View

Do you think we will ever find MBEs (mechanism based explanations) that will replace law-based ones in physics?

Although fruitful in special science's like biology that also accept the external reality hypothesis , MBEs have not found fruitful ground in physics. Not that funded research has ever been...

21 November 2023 686 1 View

What is the Rank difference method of computing coefficient of correlation?

21 November 2023 7,427 2 View

What are Differentiate between Levels X treatments designs and factorial designs and their uses in educational research studies?

21 November 2023 208 0 View

What is the prevalence of HEV in vulnerable groups?

by country by group

21 November 2023 9,397 1 View

What tools can be used to make Double Kink dislocation in BCC alloy?

We are working on kink nucleation and migration using NEB method in LAMMPS. To provide intermediate replicas we need to make kink pairs. Can anyone suggest a method or tool that can be used to...

20 November 2023 8,259 4 View

What is a conference paper?

20 November 2023 2,729 1 View

When men are in vulnerable based on gender concept?

Gender when faces livelihood problems called vulnerable.

20 November 2023 8,571 0 View

How to best analyse a sensory evaluation records?

Hello all, Hope this message finds you in good health. I am trying to analyze sensory data "9-hedonic scale" using RStudio, What is the best analysis to use, and is there a script I can...

19 November 2023 2,948 3 View

Is ISABB journal of health and environmental science a predatory journal?

I am not sure if the journal authencity

17 November 2023 4,951 3 View

Need help in calculating saturation indices of some Arsenic-bearing minerals. Anybody working on arsenic geochemistry using PHREEQC or similar models?

Kindly let me know if anybody has expertise in the geochemical modeling of arsenic using PHREEQC or similar geochemical models. I need to calculate the saturation indices of some arsenic-bearing...

17 November 2023 6,615 2 View

I have heard from some scientists recently that diamond can be in ophiolitic ultramafic rocks. Do you think it can be correct?

I want to know more about diamond bearing ultramafic rocks.

17 November 2023 4,020 2 View

What is the meaning of negative coefficients of partial determination (i.e., partial R-squared) in GLM?

Dear Community, I am interested in reporting the relative importance of my predictors in a GLM (Gamma distribution, log link). In other words, among significant predictors, I would like to know...

17 November 2023 8,046 3 View

Immunofluoresence of membrane protein, but I got strong signal from the nuclus, do you have similar experience? Why?

I immunofluresence stained a membrane protein, but after 594 goat-anti-rabbit secondary antibody incubation, I found strong nucleus signal under microscope. Do you have any idea why this happened?...

16 November 2023 4,627 1 View

When we required crisper over homologous recombination ?

I am happily using homologous recombination for candida genetic experiments then suddenly a question came in my mind why some people use crisper and other methods for gentic methods and major...

16 November 2023 2,893 0 View

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