Established cell cultures that have the potential to propagate indefinitely. | Contact experts in Cell Line to get answers
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We were trying to generate Table 1 from Phenix and got errors, saying that our unmerged data and merged data have different values for unit cell and space group. The numbers for the unit cell are...
21 June 2024 6,873 0 View
How can I prepare 4 aminomethyl benzoyl chloride from 4 aminomethylbenzoic acid and thoinyl chloride and which method and what solvent I have to use ?
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Hi, I'm working on the immune cells in the heart and have a problem with CCR2 marker. I did some research and found there's CCR2+ monocytes in heart failure mouse model. When I analysed CCR2...
09 June 2024 7,632 5 View
Hello everyone, I am preparing to take WB for Vimentin and beta-actin. Vimentin's molecular weight is 54KD, and beta-actin's is 45KD. Since their molecular weights are nearly similar, I am...
09 June 2024 2,411 1 View
We are an electrophysiology lab out of Milwaukee. Recently, due to ice buildup, a small crack in the door of our -20 freezer resulted in all of the freezers contents reaching room temperature...
07 June 2024 2,532 1 View
Hello everyone, I would like to know if any of you ever tried to assess the yeld of RNA extraction with the mirVana™ miRNA Isolation Kit, without phenol from ThermoFisher. I am experincing some...
07 June 2024 6,054 4 View
CD- cluster of Differentiation
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Men, remember, feel good as a result of having sex. Women want to feel good before having sex. (Kramer & Dunaway)
05 June 2024 1,898 7 View
I have over 40 participants that I have already analysed and written about. Now I need to do a synthetic case study. Can you tell me what this is? Or is it best to do a deviant case study?
05 June 2024 6,069 0 View
“Theoretically, the computation of passive motion requires a comparison between an internal estimate of the sensory consequences of active self-motion (i.e., forward model) and the actual sensory...
04 June 2024 3,354 0 View
I am trying to pulldown my GFP fused protein of interest using ChromoTek GFP-Trap® Magnetic Agarose. My protein is not binding to the beads. Expression of the GFP-fusion protein has been...
03 June 2024 8,601 0 View
My transduced cell can resist Puromycin and 99% express GFP, but it cannot show me the difference in the knockdown. What could be the possible reason?
01 June 2024 813 2 View
Reference for BMEL cells : Inducible differentiation and morphogenesis of bipotential liver cell lines from wild-type mouse embryos Hélène Strick-Marchand, Mary C Weiss doi:...
30 May 2024 7,472 0 View
Dear all, This is a microplastic image from the fish gut, and I am having difficulty identifying this shiny particle. I am guessing it may be a film particle. If someone knows, please guide...
28 May 2024 6,039 3 View
In my study i have 2 groups of patient (diagnosed with stage 1 and 2 at the beginning of treatment), where dependent variable is "lesion volume", which was measured before and after treatment....
28 May 2024 10,339 11 View
I have come across literature that RAW 264.7 are M1 biased, do they express CD86 without LPS/ IFN stimulation?
28 May 2024 6,602 1 View
Why we checking the GCA of a parent during hybridization program?
27 May 2024 826 1 View
I used regular freezing media (90% FBS and 10% DMSO) for cryopreservation of isolated human mature adipocytes from adipose tissue in -80 degree. When I freezed the cells, the number of cell was...
27 May 2024 8,035 0 View
Comprehensive Management of Acute Abdomen: Strategies for Ra... What are the postoperative considerations and potential complications following surgical intervention for acute abdomen?
25 May 2024 3,330 1 View
But I don't get hydrogel. Its FTIR is different from the FTIR presented in the articles. Can anyone help me?
24 May 2024 10,116 2 View
Advancements and Challenges in Delirium Prevention and Manag... What are some future research directions identified for advancing our understanding and management of delirium in critically ill...
24 May 2024 1,914 1 View
Greeting, I am using PCL and IPPDI to produce the Thermoplastic Polyurethane with different ratio. I would like to calculate the amount of PCL and PPDI that need to produce 0.2 and 0.4 ratio of...
24 May 2024 9,723 0 View
The literature regarding the effects of vit c in diet on HSP 70 is scant,particularly in the gills of fish. Either the vit C supplementation in diet increases or decreases HSP 70 in the gills of...
20 May 2024 7,611 0 View