Established cell cultures that have the potential to propagate indefinitely. | Contact experts in Cell Line to get answers
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Hi everyone, I am performing soft agar colony formation assay on Jurkat and activated T cells. The agar concentration are: 0.6% (top) and 0.3% (bottom). For both cell types, I am titrating...
24 May 2023 7,624 0 View
Hi, researchers. I kindly need help for crystallinity calculations. My data is in word document and I dont have software inorder to identify peak values. How do you calculate crystallinity in this...
24 May 2023 6,987 2 View
Different primary methods of collecting data.
23 May 2023 5,045 3 View
I want to prepare chitosan solution for my work. I need a method to dissolve chitosan flakes in the acetic acid. Please suggest protocol for the same.
23 May 2023 3,797 5 View
I have docked my protein with a ligand. When I am checking the polar and non polar contacts, I am getting the residues different in Pymol and LigPlot. Can someone please help me with this.
23 May 2023 9,311 4 View
Hello Everyone I am using 0.25% tryspin to digest my cells for passage/seeding. However, I just notice that the cell quality is not good based on appearance (e.g. many rough cells and clusters...
23 May 2023 2,911 3 View
To evaluate neurite growth in vitro on neural scaffolds with Schwann cells, what cells can be used besides PC12?
22 May 2023 7,389 2 View
This error has occurred: Logging screen output to file "Trichoderma.3genes" Exiting mrbayes block Reading mrbayes block Setting outgroup to taxon "AH123_Fusarium_oxysporum_Root"...
21 May 2023 9,951 1 View
Mtt assay
21 May 2023 702 2 View
I am working on a project looking at the cytotoxic effects of lipid oxidation products on caco-2 cells. I would like to include a sample group with added antioxidants as a 'negative control'. What...
19 May 2023 1,742 2 View
I used the Pierce Silver Stain kit, a Criterion XT 4-12% Bis-Tris Precast Gel, and fresh MES running buffer. The gel was run at 200V for ~45 minutes. Samples were denatured in DTT. This is from a...
19 May 2023 468 0 View
I just established a cell line after long-term term culturing and would like to screen for contamination. I only have Hoechst 33342, and I would like to know whether it can be used for mycoplasma...
19 May 2023 9,203 1 View
Hi everyone, I am measuring the IL-6 secretion in the J774.A.1 cell line upon the activation with a TLR1/2 ligand. In my previous studies, everything seemed normal. But recently I have had some...
19 May 2023 2,349 2 View
Why would granite have larger crystals than igneous rocks and which is the type of weathering that is caused by water ice and wind?
18 May 2023 5,365 1 View
Does anyone have a live/dead stain that works for HFF cell lines?
17 May 2023 1,581 2 View
Dear all, I am approaching EBV culturing, both clinical isolates and laboratory strains. I found different options for laboratory strains on ATCC (e.g., Strain HR1 (clone of P3) or Strain B95-8)....
17 May 2023 7,321 0 View
I would like to know if you could help me with a theoretical problem. I know the proportion of Europeans, Africans, and Amerindians in the population of Rio de Janeiro. I am studying the frequency...
17 May 2023 4,504 1 View
Hello research community. I am growing some T-cells expansions using the Immunocult XF medium and immunocult XF CD3/CD28 activator. Until day 3 of expansion cells were growing good and they looked...
16 May 2023 2,696 0 View
How many naïve T cells we can get from a C57BL/6J mouse spleen and lymph nodes? or in other word, what percentage of the cells from spleen and lymph nodes are naïve T cells?
16 May 2023 1,473 3 View
Hi everyone I'm trying to clone a gene sequence. I have designed specific primers on the gene sequence, but the PCR has more and more bands. I tried to clone, and later did a PCR with both...
15 May 2023 5,339 3 View
I have read a recent article that says that the intake of dietary fiber will reduce the risk of breast cancer. Is there any confirmed results on this?
15 May 2023 459 3 View
I want to add a GST tag to my protein. Looking for commercially available expression vector.
15 May 2023 5,031 2 View
morphology as well as anatomy
15 May 2023 4,678 0 View
As a final project for a college course, we need to do a primary culture of non parenquimatic liver cells from mice. In our first try we obtained a great amount of white blood cells instead of...
15 May 2023 9,549 2 View