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Questions related to Culture Cells
My cells namely Kasumi-1 and Thp-1 previously was healthy and growing at a steady rate but just a few days ago it appeared to have been contaminated with the same bacteria that plagued me...
26 April 2021 7,196 4 View
Please, can anyone please help me with this issue? By BN-PAGE and western blot on DDM-solubilized mitochondria, isolated from cultured cells, I found that in treated cells the amount of CV...
06 December 2020 8,033 2 View
Hello everyone, does anybody know whether it´s possible to purchase commercially available Influenza A virus which is tagged with any fluorescent dye (i.e. GFP, mCherry, whatever...)? Or does...
01 December 2020 9,751 1 View
Hi everyone, Currently I'm culturing cells in a 3 mg/ml fibrin hydrogel. However, the cells either destroy the gels (without any anti-fibrolytic compounds) or compact the gels when anti-fibrolytic...
02 August 2020 8,438 1 View
Hi, I'm working on exosomes isolated from cell supernatant of cultured cells. Briefly, I accumulate 100 - 200 ml of supernatant and process it until a concentration in a final volume of 50µL....
21 July 2020 2,009 7 View
In our lab, we use Corning 3903 White 96 wells plate to culture cells and use Celltiter-Glo (Promega G7570) to test cell viability, I wonder if we could use normal clear 96 wells plate, such as...
20 July 2020 8,397 3 View
Hello everyone, I would like to observe the effects of a recombinant protein on in vitro cultured cells. For this reason, I need to know a concentration at which recombinant proteins can be used...
29 May 2020 5,611 3 View
Hello, I am interested in this research field. I have the prototype of my microfluidic chips, but I am having a hard time finding literature that says exactly how they incubate their chips (with...
22 April 2020 3,661 5 View
Can someone please answer me this. I am storing the cells dissolving it in the freezing media(culture media + 5% DMSO) at -80 deg C. Is it safe to do?.
03 April 2020 5,038 3 View
Hi guys, I ave to study the Vglut1 puncta density/clusterization in cultured cells and see any differences between 2 different conditions. I noticed that in treatment B Vglut puncta appear...
30 March 2020 6,428 1 View
If I add exosomes to cell culture, how long until they are taken up by the cell culture cells? And then how long until they cause changes? Hours? Days? Thanks! Allie
24 February 2020 7,215 2 View
I have been trying to culture cells from the tumour tissue sample I obtained. I homogenized the tissue sample and then seeded the sample into culture medium. But no result or there was a...
22 February 2020 4,852 4 View
02 February 2020 2,896 4 View
Hi, I am designing an experiment for intracellular cytokine staining. My question is, after culturing cells for 24-48 hours, can I use trypsin-EDTA on splenocytes to dettach cells? Any input will...
02 February 2020 9,743 2 View
I want to prove a kind of protein in medium is from exocytosis but not lysed dead cells. I can detect the protein in medium by ELISA(no band in Western blotting), but I'm not sure where does the...
21 January 2020 9,110 4 View
hello everybody, I would like to measure Hepatic insulin sensitivity both in vivo and cultured cells. Thus, I am looking for new methods other than traditional previous approaches such as (glucose...
22 December 2019 6,857 1 View
12 December 2019 5,192 1 View
When culturing cells from small pieces of Wharton's Jelly 1-3cm2 in size, what media is successful in promoting the cells to migrate out of the Wharton's Jelly matrix and adhere to the culture...
12 December 2019 2,167 1 View
Would it be OK to culture cells in 96 well plates for over 72 hours? We need to run an experiment which involves cell seeding then treatment after 12 hours. The cells should then be incubated with...
14 November 2019 9,284 4 View
11 November 2019 9,071 3 View
I am aiming to measure the concentration of a drug inside of cultured cells, what would be the best method for cellular lysis with solid phase extraction and HPLC-MS as downstream processes.
11 November 2019 5,608 2 View
Can this media be used to culture cells from the tissue?
25 October 2019 4,028 2 View
I am stimulating my cultured cells and recording calcium activity as fluorescence change using live-cell calcium imaging. Though I am more concerned about the decay of the signals, for which I am...
24 October 2019 4,148 6 View
Hi everyone. I am working on primary culture of OSCC. After 25 days of culture cells have grown like these. Can anyone help me in identifying the type of cell please?
22 October 2019 4,597 3 View