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Questions related to Molecular Cell Biology
which viability assay is most suitable to check the live dead cells seeded hydrogel, can anyone suggest to me, which method MTT Or calcein-AM stain assay is best to check viability, that it...
17 February 2021 4,726 3 View
Actually i am synthesizing urethane dimethacrylate by no-isocynate route but unfortunately after washing ,driying and evaportaion not able to remove Methacrylic anhydirde. In FTIR i mention the...
04 September 2020 989 2 View
I am cloning a small fragment into my vector harboring Cas9 gene using BplI enzyme for restriction digest. I linearized my vector and treated with rSAP before performing gel extraction to excise...
23 June 2020 7,030 8 View
How does the awareness that plants are intelligent can help plant scientists? May it be a new school of thought in realm of plant studies?
30 May 2020 2,708 3 View
Hello, If the gene our interest to amplify does not follow any criteria in designing primers how to proceed with that sequence? If the gene sequence is1000 - 1500 bp. Thank you, Ramanjaneyulu
15 May 2020 1,534 15 View
Hi all, I used the Thermo-ANZA series for overnight digestion of plasmid backbone to ensure clean digestion without any problem. How about NEB? The website said all NEB "time-saver enzyme"...
25 March 2020 7,758 2 View
Dear expertise, 1) I have some trouble to get reproducible results of phosphorylated protein from western blot. Please give me some ideas and suggestions to get the perfect/reproducible results...
16 March 2020 6,831 4 View
I use the same primer set and same conditions. The first PCR product from cDNA gave about 800 bp. Reamplification of the template gave 700 bp. A second amplification gave 600 bp. Interestingly, I...
25 February 2020 4,466 8 View
Doing an in vitro study with nanostructured lipid carriers with vitamin D and with just vitamin D alone, and just trying to figure out the optimal incubation time so I don't stop it too late...
24 February 2020 9,176 2 View
From a pure biology perspective, when a pathogen affects a host, what comes first, the effect of the toxin (effector protein) on the pathways or identification of the structure of the toxin by the...
11 February 2020 7,025 6 View
In our experimentation, end point is quantification of NK cells after treatment period. Different markers like CD25, CD69, CD16 etc., were given In literature (there is no uniqueness in marker...
02 February 2020 8,446 3 View
02 February 2020 3,351 8 View
Can someone please help me with the promoter sequence of a gene?. I am working on a HOXA3 gene and I want to know the Promoter sequence of a gene. How do I find that?. Can anyone Please give me a...
02 February 2020 5,999 2 View
A model organism is one which is studied to further our understanding of biological processes. Typical characteristics of model organisms include developing to maturity rapidly, the ability to be...
01 January 2020 2,785 6 View
Hi, I have extracted manually DNA from fungi, which is time consuming and also required a lot of chemicals. I have isolated many fungi from glaciers, i want to extract their DNA easily, good...
12 December 2019 1,851 11 View
Dear all, Good day, currently I am practicing extracting total DNA using different fungi. My main purpose is to extract total DNA and send the extracted DNA for 16S Amplicon sequencing (ITS-1 and...
12 December 2019 7,525 7 View
I need to use mouse NK cells for my research...usually I use primary NK cells from the mice's spleen but I have to kill large number of mice (SJL) to get good number of NKs... thus I want to use...
12 December 2019 3,731 3 View
09 December 2019 4,268 16 View
Hello researchers I have a fresh oral tissue samples collected by Rovers Orcellex Brush , unfortunately I can not use any kit. Please, I need trusted protocol to extract DNA from this brush...
11 November 2019 8,396 3 View
I am currently performing cell cycle analyses using flow cytometry and was wondering what a double G0/G1 peak might indicate. It has appeared rather consistently in my data and is usually...
11 November 2019 5,055 4 View
I am working with some strains of cyanobacteria isolated from Zimbabwe's Manyame catchment - I want to extract their genomic material so that I may use it to confirm their identity. In my lab I...
24 October 2019 5,264 3 View
autophagic activity suppression by Rubicon is a signature of aging. how many time it takes in the suppression of autophagic activity by Rubicon? When Rubicon functions in neurons? How Rubicon...
16 October 2019 7,684 3 View
Hi everyone, I was wondering what antibodies or pcr primers I would have to use to control my fractions in a polysome profiling experiment ? A quick search highligthed antibodies against small...
10 October 2019 4,802 1 View
10 October 2019 7,239 3 View