Established cell cultures that have the potential to propagate indefinitely. | Contact experts in Cell Line to get answers
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Good evening, I want to separate lymphocytes from human blood then culture these cells for stimulation and determine the fractions of T, B and NK cells in samples. Is the attached protocol is...
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Dear colleagues Hello I am studying esophageal cancer KYSE-30. I saw these in the cell culture media after 24 hours of thawing. Do you think it is cellular debris or contamination? If it looks...
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How to prepare yeast sample for SEM
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Dear all, I have done FTIR to get results in favor of Microplastics. I got many peaks in different wavenumbers (from 400-4000 cm-1). Please suggest to me, how can I analyze the data. How would I...
06 July 2023 1,456 3 View
I had plated my cells on 3rd July, and checked them yesterday on 5th July. usually after two days I can see confluent enough growth, around 60% since I plate them 4:10 ratio. But yesterday, the...
06 July 2023 7,219 5 View
I have data set of water level in my study area. However, i don`t know how to used it to make the DTM using ArcGIS software.
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After transformation and inoculation, I did colony pcr and got good bands, I selected the good bands for plasmid extraction. But after plasmid extraction when I sent my samples for sequencing, the...
06 July 2023 2,499 5 View
Book on Fish Nutrition: Protein and Amino acid Requirements
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Join, and show advocacy to the European Brain Council (EBC) which comprises nine member organizations, which are two of them are patient groups, and the other seven groups represent the research...
05 July 2023 8,594 31 View
Hi, i am getting huge backgrounds from Immunoprecipitation samples when I probe with my antibody of interest from western blot. i am using Dynabeads. Can anyone help me troubleshoot. thank you
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Hi! I am checking the structural integrity of an existing trussed tower ( 17m high ) using SAP2000. I found out that the main angle bars used are not within the provision for seismically compact...
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Should I take into account the protein tag (His/ GST) during the calculation of pI to choose which ion exchanger I should use? Or should I consider only the real target protein sequence only?
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I made a rice beer. I want to get volatile compound from it and for this I need to study it in GCMS. Can anyone tell me what is the sample procedure for GCMS?
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Recently, I did an experiment where RNA was isolated by TRIZOl reagent. Finally the concentration of RNA check by nanodrop was around 5-16 microgram/microliter. Their 260/280 ratios were in the...
03 July 2023 2,807 13 View
Hello. I have a souce of neutrons and my F4 tally value (non-normalized) in a cell is 10^3/(cm^2). I want to increase the value, for exemple 10^9 /(cm^2), without modifying the area. I used FM...
03 July 2023 2,553 1 View
Has anyone done any fluorescent-based detection assay using this machine? Is there a protocol? Which fluorescent dye among Cy3 and fluorescein will be suitable (give a high signal over the...
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I am working on Immunohistochemistry for Per1 protein expression on mouse brain coronal sections (40microns thickness, free floating). In the protocol that I'm following, it says that the DAB...
30 June 2023 2,619 4 View