Established cell cultures that have the potential to propagate indefinitely. | Contact experts in Cell Line to get answers
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methods of research of gallstone diseases (nucleation time)
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longevity, solidity, patency, reliability.... Thanks
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How does matter and energy move between each of the spheres and ways the Earth's heat energy is distributed through the atmosphere?
18 January 2024 8,051 0 View
Hello everyone, I've just realized that I haven't put the RNeasy MinElute spin column I received with the RNA extraction kit (Qiagen) in the fridge... I received them in December and I've just put...
17 January 2024 6,617 1 View
I am currently collecting 1k-100k cells through FACS sorting for qPCR and RNA-seq. Several online protocols suggest sorting cells directly into TRIzol, and I have found this method effective for...
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How can the school supervision practices in the Philippines and Indonesia be enhanced collaboratively to improve the overall quality of education in both countries, taking into consideration the...
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My sample's dissolved oxygen is decreasing dramatically.What can I do to keep it stable or increase it again?
15 January 2024 3,264 7 View
When bootstrapping CIs go through 0 there is no significant indirect effect. But what if one of the CIs is at 0? e.g. -0,007 - 0,000 or 0,000 - 0,065 Is this also considered not significant?
15 January 2024 5,040 4 View
hi again! I had never worked with Caco-2 cells before and just recently I thaw some. When I look at them I see something that I really don't know what it is. I know these cells secrete a lot of...
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I have been working with E. coli DH5 alpha competent cells to amplify my PLJM1 plasmid with an insert, but it just doesn't get amplified in the appropriate amount! The bacterial yield is too low...
14 January 2024 8,851 3 View
I'm performing conjugation assays in E.coli. I need E.coli J53 as recipient strain. This strain is not available in my university. If anyone have this strain in their lab kindly tell me. thankyou
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I want to artificially create soil organic carbon gradient in a soil by mixing externally applied organic matter for one of my pot-culture study. pH, SOC content and clay content of my soil are...
12 January 2024 9,730 5 View
Hey, I am currently doing my thesis and I am working with skin cells to detect a specific biomarker. I want to look for a biomarker in my collected media after treating them with 4 different...
12 January 2024 2,349 3 View
Hi, I extracted total cellular RNA from HEK293T cells by using a commercial kit. I left samples untreated or treated with 2ul RNaseA/T1 mixture (invitrogen) in the presence of PBS or protein...
12 January 2024 2,312 4 View
This dot appeard instead a visible distinct band. the concentration of the DNA was 30 microgeam/ml Can you help me please if this is a band or not and why it appears like that ?
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Dearest fellow scientists, I've been trying analyze some ions with ion chromatography using suppressed conductivity detection. One of the ions im trying to measure is tertbutylammonium (TBA)....
11 January 2024 960 2 View