Established cell cultures that have the potential to propagate indefinitely. | Contact experts in Cell Line to get answers
9,000 views 10,003 posts
Questions related to Cell Line
all model can be viewed as sum formula of features?
18 December 2024 2,900 8 View
Articles about machine learning applications in the circular economy are of interest. Also, what are the sample machine learning applications in the circular economy approach in the furniture...
17 December 2024 1,625 3 View
Recently, I encountered some difficulties while attempting to sort 4T1 cells. The main issue is that 4T1 cells like to stuck together, which has led to a considerably low yield of sorted single...
17 December 2024 851 2 View
Hi all! 2 weeks ago I started culturing a vial of frozen HCMEC/D3 cells from Merck. Even when the process has been long and full of uncertainty, the cells are alive, proliferating and somehow...
16 December 2024 8,262 2 View
The question explores how artificial intelligence can make education more personalized and interactive, highlighting the opportunities it offers to enhance the learning experience. It also...
14 December 2024 9,118 1 View
Hi, I am looking for a validated survey to assess the prevalence of injury and the perceived risk of injury in athletes.
14 December 2024 8,114 0 View
Recently, many AI tools have been developed for cancer research, including, but not limited to, diagnosis and treatment. Therefore, providing specific guidance may help the research community...
13 December 2024 3,769 5 View
My target gene was sequenced and the sequence was correct. WB, qp and IF experiments all proved that it was overexpressed. However, when I looked for the gene regulated by it, I found no...
12 December 2024 3,165 2 View
Hello< i would really appreciate your insight about using via for different application. I know that there is different modeling techniques. 1: Hollow via 2: Metalized via 3: hollow with metal...
11 December 2024 859 0 View
Hi everyone, I’m working on a project involving the culture of PBMCs and I’d appreciate your insights. We’ve been staining cells with CTV and activating them using ImmunoCult™ Human CD3/CD28 T...
04 December 2024 376 0 View
Hi everyone, I’ve been trying to get my review article published for over a year now, but I keep running into roadblocks. I don’t have support for open-access fees, and I’ve submitted it to more...
04 December 2024 5,691 3 View
There is no volume nor issue #
03 December 2024 3,012 1 View
All about dairy innovations.
02 December 2024 5,897 1 View
This is a result of silver nanoparticles synthesis using bee pollen and ethanol as a medium. I also used water and managed to obtain decent nanoparticles but not really in this one. I would like...
01 December 2024 2,429 0 View
Dear Professors and Researchers, I am seeking a PhD position in the fields of Artificial Intelligence (AI), the Internet of Things (IoT), or Number Theory in Mathematics. With a Master's degree...
30 November 2024 5,296 1 View
Dear Respected Scientists, Can we trust the results if MLPA data from amniotic samples for SMA indicates a ligation failure warning but shows normal peak heights in GeneMarker software? We also...
27 November 2024 8,628 0 View
I am looking for an answer related to western blot, we have mouse eye tissues which is formalin fixed and then stored in 75% ethanol, Can I use it successfully for western blot?
23 November 2024 5,618 5 View
I’m working with fibroblast cells that we plate on a carbon nanotube surface for gene transfer. At the end of the experiment I’m trying to remove the cells with trypsin (or TryplE), but it appears...
22 November 2024 6,977 5 View
Hello all, we are working on functional validation of a transposon mutated virulence genes in Agrobacterium tumefaciens. However, in the mutant, when the gene expression is analyzed by qPCR, we...
21 November 2024 6,488 3 View
I have conjugated a drug with carbon dots using the EDC/NHS method. I am seeking quantification techniques to determine the amount of conjugate in the solution, as well as the concentrations of...
21 November 2024 110 1 View
Dear All, We intended to estimate individual lipid classes (MGDG, PG, DGDG,SQDG & PC) from rice tissues using GC-MS. We extracted lipids using TLC and derivatized and ran the...
20 November 2024 4,950 2 View
I am growing mesenchymal stem cells isolated from Wistar rats bone marrow. Initially the cells were getting detached or trypsinized in the culture flasks easily. But now I am unable to detach the...
19 November 2024 737 2 View
Hello! I am planning to KO a gene that seems critical to the cell's viability by CRISPR/Cas9. The question is, how can I detect the dead cells that are truly dead by KO by Cas9 when the gene is...
18 November 2024 1,113 6 View
Hello, I am trying to study synapses and try to stain synaptophysin and synapsin on postmoterm rat brain tissue. But so far have completely no siangls under confocal (I am expecting to see...
18 November 2024 8,068 1 View