Established cell cultures that have the potential to propagate indefinitely. | Contact experts in Cell Line to get answers
2,252 views 6,669 posts
Questions related to Cell Line
I am using CD3/CD28 beads (gibco) for activating CD8 T cells (1:1 ratio). The T cells are isolated via a CD8 isolation Miltenyi column from freshly collected PBMCs. In 4 different donors I have...
30 June 2023 2,538 1 View
I have to perform an IHC and I have three different antibodies to test. We already have one made in rabbit and another made in mouse, both antibodies react against human. We have to test a third...
29 June 2023 4,746 4 View
Hi, I'm new to this forum and I have a question for you. Here's my question: My goal is to quantify the fluorescence intensity. Let me explain: I am currently using zebrafish as an in vivo model...
28 June 2023 3,927 4 View
I need to evaluate the antioxidant and cytotoxicity activity for polymeric material . It doesnt dissolve in common organic solvents even dmso. Is there is a way to do such test!
28 June 2023 8,747 3 View
If so, please share it. It has been announced today by Clarivate
28 June 2023 7,321 2 View
I am running a gel electrophoresis to check mycoplasma positive samples using PCR, however only positive control shows up. I tried new master mixes and diluting the samples but in all cases only...
28 June 2023 3,434 3 View
Hei everyone, I ll start here a discussion for which I can't find any answer online. We are facing an issue with the water that we use in PCR and qPCR. The problem is that when we use 16S...
28 June 2023 718 7 View
Hi everyone I am currently investigating the effect of iron deficiency on neuronal cells (SHYSY5Y to be exact). For this, I need to create an environment of iron-depletion in-vitro. While past...
28 June 2023 1,882 0 View
Also would like to know the ExpiCHO parameters like ellipticity/ circularity and max/min. diameter to count the cells via automated cell counters. Thanks for the help!
27 June 2023 869 0 View
Do Monte Carlo or Generalize Linear Model do the same thing? What are their difference? Which is best for a count data and why?
27 June 2023 3,678 2 View
Dear Colleagues, We routinely use MTT assay to test the cytotoxicity of compounds. Recently, however, our background values of some cell lines (e.g., MCF7, MDA-MB-231) are shallow, while other...
27 June 2023 4,633 5 View
Which one is better as a housekeeping (B-actin and GAPDH) gene for RT-PCR in hippocampus?
27 June 2023 7,499 4 View
I seeded two flasks (T-12.5) in two different CO2 incubators. They contained the same number of cells, the same media, etc. The one-seeded in the rarely used incubator had an abnormal morphology (...
26 June 2023 4,985 10 View
Hello, I'm working on drug delivery in cancer cells. For that, I prepared slides of adherent cancer cells for confocal microscopy. I fixed the cells with 150 ul of 4% paraformaldehyde for 10 min...
26 June 2023 5,316 3 View
I would like to induce BM monocytes into immature dendritic cell. Could anyone suggest any protocol for that? Also, is there any way to store the immature denditric cell?
26 June 2023 1,889 2 View
Good day everyone. Please I would like to treat 10 and 50μM of ATRA to LX2 cells. I understand that I am to dilute the ATRA in DMSO but I do not have a lot of experience in using DMSO. Please...
26 June 2023 5,448 2 View
I am using Mammocult media and low-adherence plates
23 June 2023 4,342 1 View
Hello! Im working with primary human MSC, I obtained them from adipose tissue, however, I've been trying to expand them unsuccessfully, I use normal T-75 tissue culture flask, DMEM with 15% SFB,...
23 June 2023 6,119 2 View
Hello everyone! I am currently doing moderation/mediation analyses with Hayes Process. As you can see the model 3 is significant with R2=.48 The independent variables have no sig. direct effect...
22 June 2023 6,522 2 View
Hello, I am planning an experiment to measure enzimatic activity, and i will take sample every 24hrs. The only problem is that substrate is non soluble... nevertheless, enzyme can still access to...
22 June 2023 9,475 4 View
I have 3 papers suitable for inclusion in my systematic review looking at high versus low platelet to red call ratio in TBI, and want advice as to whether I can combine their estimates of effect...
22 June 2023 7,237 2 View
I'm trying to find an ELISA kit that can measure NFkB using 24 well culture plates at a density of 5X10 4 cells/well. Thank you
21 June 2023 1,550 3 View
RT–PCR is a variation of PCR, or polymerase chain reaction.
21 June 2023 1,703 3 View
how can we insert a gene into a vector without restriction enzymes to cut the vector we need restriction enzymes.how can we insert the gene of interest without them?
20 June 2023 9,337 4 View