Established cell cultures that have the potential to propagate indefinitely. | Contact experts in Cell Line to get answers
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I'm doing a protocol in which I plate my cells in a 12-well plate with complete medium (i.e. DMEM/F-12 WITH 5% FBS), let them reach ~80% confluency and then I change the complete medium for a...
11 January 2024 8,187 5 View
Hi everyone! I tried to perform a classic One Way Anova with the package GAD in R, followed by a SNK test, which I always used, but it didn't work with this dataset, and I got the same error for...
11 January 2024 7,617 1 View
Hi Can you please explain to me about the need to do the PCA analysis in molecular dynamics simulation? Thanks alot
10 January 2024 185 4 View
I'm following a protocol to bind fluorescent ligand to receptors expressed on cells in a microplate. The protocol includes fixing cells with 4% PFA for 20 mins. This prevents cell loss during...
10 January 2024 7,468 7 View
In your opinion, what are the key challenges faced by lecturers in delivering quality teaching?In your opinion, what are the key challenges faced by lecturers in delivering quality teaching?
10 January 2024 1,413 3 View
𝐑𝐞𝐦𝐞𝐦𝐛𝐞𝐫𝐢𝐧𝐠 𝐆𝐢𝐚𝐧𝐭𝐬 𝐢𝐧 𝐒𝐮𝐫𝐠𝐞𝐫𝐲 & 𝐒𝐜𝐢𝐞𝐧𝐜𝐞𝐬 Ｊöｒｇ Ｒüｄｉｇｅｒ Ｓｉｅｗｅｒｔ * 𝟎𝟖.𝟎𝟐.𝟏𝟗𝟒𝟎 - † 𝟎𝟗.𝟎𝟏.𝟐𝟎𝟐𝟒 𝐑. 𝐈. 𝐏. to my former Teacher and Chairman #Siewert #surgery #chirurgie #science #wissenschaft
10 January 2024 5,839 4 View
Hello! I want to measure the osmotic pressure/osmolarity of some tissue culture media. I have a freezing point osmometer which I use for liquid media, however I don't know if this will be...
10 January 2024 3,856 1 View
We have soil quality indices to judge the performance of soils concerning a certain management goal (yield of crops, in most cases). I was wondering, is there any comprehensive and holistic Crop...
09 January 2024 6,634 6 View
I dipp the electrode in 3M KCl solution but it's still so milky.please give me some suggestions to clean it.
09 January 2024 448 3 View
How is ammonia changed to nitrogen and can atmospheric nitrogen being converted into ammonia without using bacteria or other organisms?
09 January 2024 2,750 6 View
I have three RNA-Seq datasets of the same tissue and want to analyse them on Galaxy. My initial literature survey gave me the idea that I can merge the three datasets if they are from the same...
09 January 2024 7,324 7 View
I am interested in understanding the location of the largest Listvenitic type of gold deposit in the world. Could you kindly provide information or direct me to resources that can help me identify...
08 January 2024 5,043 2 View
Tuesday, Oct. 3, the Nobel Prize committee announced the 2023 physics prize, recognizing Pierre Agostini, Ferenc Krausz, and Anne L'Huillier "for experimental methods that generate attosecond...
08 January 2024 8,259 2 View
Hi, I need to perform immunofluorescence staining (IF) of primary human keratinocytes, melanocytes and fibroblasts from skin outgrowths. Can somebody advice which marker to use for these 3 cell...
08 January 2024 7,661 0 View
I am doing research on 3D-printed skin substitutes, however, I don't see any related articles reporting this. Any suggestions for reading? Thanks
08 January 2024 4,113 0 View
Hello, can you please guide us on the hydrogeling method for decellularized skin?
07 January 2024 5,274 0 View
Dear researchers kindly let me know is it will be feasible to culture blood cells in animal cell culture lab and put the isolated cells to grow in cell culture medium in the same CO2 incubator in...
07 January 2024 1,134 5 View
Do Not oPERATE ON ME. u dONT hAVE sOMETGING tO oPERATE iF FOR nO Reason For Operating It. Why Are U Trying To Urgently So Badly Trying To Operate The Machine PIK ?@@@>@@>>
06 January 2024 7,689 0 View
I would like to convert the oxygen content given in mg/l into the unit of measurement ml/l and vice versa. How do I do this?
05 January 2024 1,087 4 View
Hello everyone, I need advice on improving the quality of my coronal slices obtained from PFA-fixed mouse brains using a cryostat. As You can see in the attached image, slices are horrible! Any...
05 January 2024 6,032 2 View
We used mouse cell line HEI-OC1 and checked OCTN1 expression by WB, then we did IP cell lysate with anti OCTN1 antibody and IB with OCTN1 antibody, surprising! The band is way above IB with WB (30...
05 January 2024 6,886 0 View
Hi Everyone, What should be the cisplatin doses for DRC in MC38 cell line inoculation in C57/BL6 mice? Best, -Abhijeet
05 January 2024 8,698 0 View
What materials can be used in photonic crystal fiber sensors to withstand and measure high temperatures? (more than 100 degree centigrade)
04 January 2024 4,320 7 View
Hello, I have a problem with staining the mouse brain. These days, my professor told me "There is something wrong with your images. I think you must have made the mistake in perfusion or...
04 January 2024 9,916 3 View