Established cell cultures that have the potential to propagate indefinitely. | Contact experts in Cell Line to get answers
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Covid-19 vaccination can induce multiple sclerosis via cross-reactive CD4+ T cells recognizing SARS-CoV-2 spike protein and myelin peptides Qiu, Y.; Batruch, M.; Naghavian, R.; Jelcic, I.;...
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Is there a special reason, why the term "superficial cervical ansa" (Ansa cervicalis superficialis) was removed from official anatomical terminology?
05 June 2023 8,357 4 View
It seems that some data are contradict to each other. The XRD assays shows the ZnO NPs have a size of X nm; where their DLS detection showed that these particles are in average of Y nm. Yet their...
04 June 2023 7,859 6 View
I tried to troubleshoot it but still no use can anyone help me with this issue, ketone double bonds are missing in ligand map
03 June 2023 823 0 View
Hello dear researchers Is it possible for someone to help me in the field of separating the hypothalamus by sending a video? Because the theory and seeing the atlas could not help me much.
03 June 2023 5,717 2 View
Presently, I am focusing on an experimental metastasis model that utilizes 4T1 breast cancer cells. We typically trypsinize the cells from the dish and then pipette them to form a single cell...
01 June 2023 8,204 0 View
I'm looking at co-culturing neutrophils and myoblasts, along with co-culturing of the conditioned media but these cells require different media.
31 May 2023 9,178 1 View
Hi, I have run a couple of Western blots for tyrosine hydroxylase in mammalian SH-SY5Y cells and have noticed 2 distinct bands rather than just one, as is shown in most of the literature. Some...
31 May 2023 8,025 2 View
Good time, dear, I need a journal article clarivate about cancer, immunology, and molecular biology and is easy to accept. PhD student and final year thanks and best regards
30 May 2023 7,272 3 View
How to synthesize the neurotransmitter acetylcholine?
30 May 2023 1,460 1 View
Three-year Ph.D. positions in Neuroscience available @ University of Verona (Italy) for 2 projects Supervisor: Mirta Fiorio 1. Ph.D. position for the project “The cognitive-motor interplay in a...
30 May 2023 5,300 1 View
Hi, I'm trying culturing monocyte, isolated from PBMC's to differentiate them in macrophages. I obtained a buffy coat from a healthy donor, I freezed most of cells in freezing solution...
29 May 2023 9,370 4 View
I am a final year medical student and have already participated in systematic reviews, case reports and other types of articles. I am interested in expanding my experience as a researcher If...
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Water Analysis: pH, E/C, TDS, TOTAL HARDNESS, Nitrite, SO4, CL-, NH4, COD CU, CD, Pb, Zn, AS, CO, MN, Ni,Cr, Hg, Fe
29 May 2023 2,836 3 View
Hi, I have NTC contamination in real-time PCR. I checked all suspicious items such as primers, master mix, and distilled water, and also the workspace. but unfortunately, I can not remove the...
28 May 2023 7,815 6 View
Dear colleagues kindly help me out
28 May 2023 5,493 4 View
My target gene fusion with Flag , I detected target protein overexpressed in mammalian cells , but i can not detect Flag. The construct's sequence is correct withou frameshift. What is the reason?
27 May 2023 1,003 4 View
Hey I work on my thesis, but i have some problem I know the result is not good, but something is happen, i dont know where/what? I work on HERC1 520KD, I prepare lysate then did CO-IP with HERC1...
26 May 2023 1,925 1 View
Now that Carestream/Kodak Biomax MR film is no longer available, I am looking for a replacement film for 14C-mouse brain autoradiography that optimizes spatial resolution. There are a host of...
26 May 2023 5,756 1 View
Is there any information available regarding the sensitivity of capto core resin to pipetting? Is there a risk of the resin or beads breaking when inserting the pipet tip for sample mixing?...
26 May 2023 8,551 0 View
In my research, I performed PCR to identify a marker linked to the R-gene in my samples. I experimented with various primers and finally achieved my desired band size through gel electrophoresis....
26 May 2023 6,904 8 View
dilution factor, phosphate buffer, magnesium sulphate, calcium chloride, ferric chloride and sodium sulphite
26 May 2023 2,628 1 View
We were doing western with cell lysate and used 5% BSA in TBST for blocking. Also, primary and secondary dilution of antibodies has been prepared in BSA in TBST. With this, we are getting a...
25 May 2023 5,500 6 View
Can someone explain the difference between a whole cell lysate sample, input sample, and eluate sample when you are running an IP via western blot?
24 May 2023 2,415 4 View