Established cell cultures that have the potential to propagate indefinitely. | Contact experts in Cell Line to get answers
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We are culturing hESCs using TeSR AOF kit and passaging with Accutase. The cells were in good condition, but one day after passaging the colonies suddenly formed weird shapes and every day there...
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When exosome-depleted FBS is not available. Also, ultracentrifugation is not available for manual preparation of Exosome depleted media. Any alternative options to avoid FBS exosomes?
08 August 2023 3,929 1 View
Hello, I'm a newbie to statistics and I'd like to know which test I should use. For my experimental design, I seed two wells for control siRNA and two wells for KD siRNA. After siRNA...
07 August 2023 4,617 5 View
I need to convert the fg/ml, and ng/ml limit of detection into IU/ml. What is the formula for converting fg/ml, ng/ml limit of detection into IU/ml?
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Hello! I am performing a lentiviral transduction on human fibroblasts for the very first time. The vector I am trying to insert contains puromycin and ampicillin resistance. How much ampicillin...
07 August 2023 5,473 4 View
I'm interested if a protein is being degraded. My idea is to western blot my samples, add an antibody for ubiquitin, image, wash off the antibodies, add an antibody for the protein of interest,...
05 August 2023 2,958 2 View
We are purifying custom-made antibodies from frozen chicken serum, and when thawed it turned into the gelatin-like substance on ice. So, their erythrocytes are nucleated and then I treated serum...
04 August 2023 2,116 3 View
I want to quantitate apoptosis in vivo. I can make a tissue slide and do the ApopTag assay. However I do not have a program for counting ApopTag positive cells. How to count ApopTag positive...
03 August 2023 7,418 3 View
I'm researching platelet-derived growth factor signaling, and recently ran into a big problem. Specifically, I am determining whether a particular peptide can activate the PDGF receptor. My...
03 August 2023 6,710 1 View
Hi everyone I have a problem with Histag protein purification. I have this construct with his tag on N-terminal. I went for small-scale expression and I had some good bands. However, on a large...
03 August 2023 4,845 4 View
I never handle the SHSY5Y cell line before, so it is a new thing for me, as far as I observed for these past five days, their growth is really slow. Is it normal? I use the complete media of...
03 August 2023 8,584 6 View
I am asking this as all questions that I can find concern more basic and widespread detachment problems but ours is recent and seems to occur at a specific passage. We have multiple iPS cell...
02 August 2023 2,050 1 View
Dear community, I have found that sensors with the smallest pixel size of 560nm are produced by Samsung and Omnivision. However, I cannot find a way to direct purchasing of these sensors. If...
02 August 2023 5,959 0 View
I am currently optimizing a RPA-based protocol. As everyone knows, this nucleic acid amplification technique is based on isothermal amplification (around 37-42˚C) in combination with recombinases...
02 August 2023 1,780 3 View
A batch experiment was conducted to study the effect of temperature (20 and 35ºC) on the adsorption of arsenic on soil having high clay, organic matter and free iron. The adsorption increased with...
01 August 2023 208 6 View
I have a target heptad sequence containing amino acids Aib, Phe, Ala and Trp at different positions. What can be the standard protocol to follow while coupling the Trp amino acid and the cleavage...
31 July 2023 3,645 0 View
I have done a number of MTT assay's for a study I am a part of, twice the results showed a very low viability and this crystal formation. I did a later assay following the same procedure, using...
30 July 2023 7,982 2 View
Hi, I’m trying to culture sk-n-sh neuroblastoma cells. After 8th day of the culture cell morphology disappears and the accumulated structure appears (Please see the photographs). Could anyone make...
30 July 2023 4,934 0 View
I came to know that systematic review at which qualitative studies are included is known as Qualitative evidence synthesis. i wanted to know the difference in methodology between both studies. Is...
29 July 2023 9,695 0 View
20-25 larvae (96h) were randomly selected, washed 2-3 times with PBS, and then stained with AO(5ug/ml) and DCFH-DA(10uM) stain for 30 min separately and then washed with PBS 2-3 times to remove...
28 July 2023 9,625 4 View
If I am working on dealing with sadness as being stigmatized as "sinful/immoral" culturally in Muslim societies that made a roadblock to the process of sad emotion regulation psychologically,...
28 July 2023 9,731 6 View
Does the use of electric cars affect the current consumption of oil and coal?
28 July 2023 2,425 1 View
Can you suggest appropriate solvent or modification
28 July 2023 8,678 2 View