Established cell cultures that have the potential to propagate indefinitely. | Contact experts in Cell Line to get answers
8,991 views 10,003 posts
Questions related to Cell Line
Hello everybody, at the moment im trying to isolate and subsequently expand T-cells from cryopreserved bone marrow mononuclear cells. But I do have problems keeping them alive for longer than 24h...
last month 1,406 4 View
Possible Research Areas: Integration of Artificial Intelligence (AI) in Teaching and Learning Impact of AI Tools on Teachers’ Professional Development AI-driven Instructional Strategies and...
last month 1,230 0 View
Hello everyone, I am currently working on tumor immunology and need to perform ex vivo expansion of PBMC-derived T cells. While searching the literature, I found that many different media are...
last month 3,164 2 View
Hello everyone, I am currently in my third year of a BSc (Hons) Computer Science with AI degree. My long-term goal is to pursue a research career in Computer Science, especially in Artificial...
last month 6,279 0 View
How can research institutions and extension agencies contribute to developing climate-resilient millet varieties and promoting their adoption under the UP Millets Revival Programme?
last month 5,471 1 View
Hi, I am preparing to repeat the experiment using GSH and GSSG standards in order to obtain a more reliable standard curve using the GSH/GSSG Assay Kit from MedChemExpress (HY-K0311), particularly...
last month 5,154 2 View
Why you keep asking me if A. Khattely was the author of a paper from 2015 (Unveiling soil degradation and desertification risk in the Mediterranean basin: a data mining analysis of the...
last month 6,784 1 View
Inside of % how we can calculate the ethanol solubility of wood as g/L?
05 August 2025 7,108 1 View
When detecting p53 in human samples by Western Blot, the antibody is supposed to show a single band around 53KDa. However, I consistently see extra bands (non-specific signals).
04 August 2025 8,654 1 View
This rainy season in Nepal is very less rainfall which is a big issue for rice growing farmers of the Terai region of Nepal
31 July 2025 2,574 3 View
Hi all! I am trying to screen synthetic receptors for their ability to bind their ligand. The ligand has a His tag (Sino 50284-M07E), so I am using an anti-His Ab for flow (Biolegend 362637). I...
31 July 2025 6,109 2 View
Could intermediate-mass black holes (IMBHs) act as catalysts for the r-process, producing gold, uranium, or other heavy elements?
31 July 2025 627 0 View
I have recently graduated with a Bachelor of Science in Biological Sciences. I am interested in pursuing a career in the field of genetics, molecular biology and molecular medicine. Is there...
25 July 2025 2,240 3 View
I would like to know what the effect of having very high mechanical properties is on the behavior of steel during hot relaxation? Will it be beneficial or on the contrary? Normally the material...
24 July 2025 2,575 0 View
i need the information or summary of the social cognitive theory
17 July 2025 9,513 0 View
I've found AI is like a 10,000 Volt Power supply or a high power chain saw. Used right, they allow you to do powerful things. Used carelessly and they can destroy you. I've taken 45 years of...
10 July 2025 6,351 3 View
In a competitive bone marrow transplantation assay doesn't the donor, competent and recipient cells should bear a unique Cd45 allele expression? I am confused by the different strategies...
08 July 2025 5,782 1 View
Dear ResearchGate Team, I hope this message finds you well. I am writing to inquire whether it is possible for users like myself—who are eager to gain research experience—to collaborate with...
07 July 2025 9,397 0 View
I use the INS-1 832\3 cell line. When I first opened the cells, it was very difficult to grow them. After they came to their senses, I started to passage and freeze them. When I open the cells...
04 July 2025 6,726 1 View
Hi all, what are the differences between these cell lysis agents: Digitonin (HY-N4000), NP40 and Triton x-100?
03 July 2025 4,560 1 View
As AI tools like ChatGPT, adaptive learning platforms, and educational bots become more integrated into classrooms and at-home learning, educators and parents are debating their long-term effects...
30 June 2025 9,013 9 View
❓ Full Question Description: In 1901, Jagadish Chandra Bose presented a groundbreaking experiment at the Royal Society of London showing that plants exhibit biological responses to external...
30 June 2025 5,651 2 View
We are looking to set up the 5TMM myeloma model in C57Bl/6 mice. Where can we obtain the cell line? Thanks
29 June 2025 1,033 2 View
Can we evaluate the phagocytosis of peritoneal macrophages in relation to Candida albicans, Gram+ and/or Gram- bacteria? Is it better to evaluate in vivo or in vitro assays? And by cytometry? If...
27 June 2025 6,944 2 View