Cell culture is the complex process by which cells are grown under controlled conditions, generally outside of their natural environment. | Contact experts in Cell Culture to get answers
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Questions related to Cell Culture
§The environment is one of the many determinants of human health. The key to man’s health lies largely in his environment, and the study of the disease is really the study of man and his...
11 November 2023 1,607 3 View
NSCs are known to form floating neurosheres in 3D culture. What are the best culture media to be used for neurosphere culture of human NSCs? Are EGF and/or FGF required in the culture media for...
10 November 2023 7,639 0 View
Hi y'all, I am running into some difficulties with qPCR (SYBR). To give y'all a brief background summary, I transfected some cells (ASO) and am running qPCR to see its effect on known genes using...
09 November 2023 9,743 6 View
Hello everyone, I'm dealing with some issues regarding resuspension and use of a Roche DNAse I that we purchased from Merck (Ref:10104159001). The product is purchased as a lyophilized vial (100...
09 November 2023 4,832 0 View
Dear all, I have been having trouble with EdU staining of primary human meningioma cells. I am finding that even with 24 hours of incubation with EdU reagent, I am seeing a very low (0-5%) EdU...
09 November 2023 260 0 View
There is no product specifications for this recombinant protein, I assume I reconstitute with deionised water? Thanks.
08 November 2023 2,377 2 View
Hello, I am hoping to conduct PCR to determine sex for a species of wild bird. I would like to used blood samples for this. I've been reading that using a lysis buffer to store the blood reduced...
08 November 2023 9,514 1 View
I am trying to purify a protein using thioredoxin tag. The clone I made is in pET32b vector having N-terminal thioredxin tag and C-terminal His-tag. I already checked the plasmid using...
07 November 2023 9,316 3 View
Is there any software that can calculate the area under the curve for a line graph? Currently, I am using "origin" software.
07 November 2023 660 3 View
I would like to perform an untargeted LC-MS analysis of the cell-free supernatant of bacterial culture to analyse all the possible metabolites present in the cell-free supernatant. Here I am...
05 November 2023 6,118 2 View
Hi! For reprogramming PBMCs into iPSCs, I understand that you first must obtain the PBMCs by a density gradient, such as Ficoll-Paque, and then you have a product of primarily lymphocytes with...
03 November 2023 4,879 0 View
Does anyone have the availability of T-cell lymphoma HT-1 and HD-Mar cell lines? Thank you in advance!
02 November 2023 2,473 1 View
Hi, im looking for mice osteoblast cell line ( not MC3T3) for in vitro work. any suggestions?
01 November 2023 8,048 2 View
In some studies (especially cancer research), a relevant cell line (e.g. endometrial cell line) is used in cell culture together with an unrelated cell line (e.g. kidney cell line). However, the...
31 October 2023 8,179 3 View
I have been having issues with my THP-1 cells for most of this year - when I try to differentiate them with PMA into macrophage-like cells about 1/3 of the cells do not adhere properly (either...
30 October 2023 2,342 2 View
Hi, Could you provide me with a successful protocol for the differentiation of mesenchymal stem cells into neural progenitor cells?
27 October 2023 5,468 0 View
I'm working on THP-1 monocytes. I thawed them last week to start my culture, but I can't count them. I count in Mallassez cells with trypan blue. I can see by microscopy that my cell density is...
27 October 2023 1,549 3 View
Hi, I'm trying to isolate RNA from cell cultures using the Trizol method. However, all my samples have low 260/280 values (~1.6). Could this be because I used chloroform and isopropanol, which was...
27 October 2023 9,867 6 View
hello, I am a second-year graduate student. When I induce the adipocytes in Six-well plates(Used with 0.5mM IBMX、0.25uM Dex and 1ug/m insulin),I find they are reticulated next day. Then they are...
26 October 2023 3,332 1 View
The title is the content. Why AIF1/Iba1 is not detected by western blot in BV-2 cell? When BV2 cells are pretreated with the drug for 1 hour and then treated with LPS(500ng/ml) for 12 to 24...
26 October 2023 5,499 0 View
Hi, I have data in form of counts (number) and I wanted to run a statistical test to compare the counts between groups to see if there is statistically significant difference between groups. I...
25 October 2023 1,500 3 View
My lab has some silver acetate or gold chloride staining on our scale and we want to remove it. Any help would be appreciated!
25 October 2023 7,909 0 View
This is for to know the how was the Regulatory Strategy in use of organoids in preclinical studies to get FDA approval.
24 October 2023 6,802 1 View
Hello everyone, I've encountered an unexpected issue while attempting to multiply bacteriophages from soil samples that were previously isolated. During the double-plate assay, I observed the...
22 October 2023 7,205 5 View