Cell culture is the complex process by which cells are grown under controlled conditions, generally outside of their natural environment. | Contact experts in Cell Culture to get answers
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Questions related to Cell Culture
I am going to prepare a stock solution (in DMSO) of Filipin III to be eventually stored and on the data sheet it says that it has to be prepared under inert gas conditions because it is sensitive...
24 November 2021 3,447 1 View
Hi guys, May I ask if it's possible to extract bone marrow from animals (rats, rabbits, dogs, etc.), then culture the extraction in vitro? After looking up some papers, bone marrow concentrate...
24 November 2021 1,079 0 View
Hi All, I'm measuring cell toxicity in different cell lines against aggregated protein solutions. I'm performing various cell viability, toxicity, and ROS level assays. Except for these, what...
21 November 2021 7,651 3 View
I was wondering where to find the viscosity of cell culturing media under 37C, As I am planning to do a microfluidics experiment using DMEM (high and low glucose) and KSFM at 37C temperature?
19 November 2021 901 1 View
Hi , I have been working with iPSC cell lines and hESCs for 6 years with Matrigel and mTESR1. I have recently started working with iPSC cells with vitronectin matrix and Stemflex media ....
19 November 2021 319 3 View
Using the DuoSet Human TGF-B1 ELISA Kit of R&D Systems (#DY240-05), which detects active TGF-B1, the protocol suggests you to activate the latent TGF-B1 of the cell culture supernatant by...
17 November 2021 3,240 4 View
Hi everyone, Can anyone please help me identify this contaminant in my cell culture? All the best and many thanks, Alfred
15 November 2021 4,379 1 View
Dear All, i would like to ask why my negative controll in cell culture fails from time to time. I am performing simple cytotoxicity assays based on Balb3t3 and L929. I am seeding the cells on...
10 November 2021 5,203 3 View
Recently, I was trying to evaluate the anti-proliferation activity of a new synthesis compound. I used the MTT assay to determine the effective concentration at first. Then, I used the effective...
10 November 2021 5,933 3 View
Hi, I would like to know if anyone has cut their electrospun material before into small round disks? Which precision device did you use (or can recommend) and did it disturb the...
09 November 2021 9,411 4 View
When we FACS sort our CRISPR knock ins the low efficiency often means we get between 50-500 cells per sample. They go into a 96-well for recovery. They survive fine after sorting but they just...
08 November 2021 4,516 3 View
Can I prepare MitoTracker aliquots, by diluting it in cell culture medium at a certain concentration, and then store them? If yes, is it better to store the aliquots at -20 or -80 °C? How long...
08 November 2021 9,303 3 View
Dear All, We are currently working on endometrial cancer organoids. We find that when passaging our organoids (in domes of BME/Matrigel), we lose a significant amount due to them sticking to our...
08 November 2021 6,419 3 View
We add amyloid β 1-42 to cells in order to create a model of Alzheimer's in cell culture. However, how can we determine exactly when Alzheimer's occurs in cells? Is there a method for this?
07 November 2021 3,682 1 View
I need to understand the difference and which is preferable?
06 November 2021 3,941 1 View
I used to work in a separate tissue culture room before, but now have to set the BSC and TC incubators in an isolated (but not closed) area of the lab. It is working well, but now we are going to...
03 November 2021 7,942 3 View
Hello I am using CHO cell to express protiens, I used GMEM media (no glutamine) with supplements I need the exact protocols to follow for suspension and adhertent cells. Thank you.
02 November 2021 5,937 2 View
I'm working on Ipomoea batatas cell culture and trying to make a growth curve. For that, I take 1 ml of cell culture and dry it overnight in an 80 0C oven. After 3-4 days the dry weight starts to...
01 November 2021 2,771 1 View
I have fixed my hydroxyapatite-coated NiTi alloy samples on which I have cell-cultured MG-63 osteoblast cells, and I have scheduled an SEM time to take pictures of the cell behavior. How long do I...
30 October 2021 6,124 4 View
Hi all, my lab currently uses thermofisher's Pierce protease and phosphatase inhibitor mini tablet. I noticed that the expiry date was back in May 2020. I keep the tablets at 4°C, and dissolve 1...
29 October 2021 1,478 3 View
I am currently working with primary human retinal microvascular endothelial cells (HRMECs) and need to grow tight confluent monolayers for TEER, transwell permeability assays, and TJ/AJ...
25 October 2021 7,659 2 View
How to choose the most appropriate phospholipid delivery vehicle (physiological delivery) for cell culture treatment?
23 October 2021 9,031 2 View
I'm now testing cetuximab on HT29. In my experiment, cetuximab had no effect at all on HT29 even at a high concentration (100ug/mL ~ 0.66uM) by viability test using WST-8. This result agrees with...
21 October 2021 5,368 0 View
I used Cytochalasin D as an actin inhibitor in epithelial cells. Crystals formed and blocked the whole well (96-well plate) at 1 µM concentration, and formed few crystals at 0.5 µM, and no...
20 October 2021 4,745 2 View