Cell culture is the complex process by which cells are grown under controlled conditions, generally outside of their natural environment. | Contact experts in Cell Culture to get answers
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Questions related to Cell Culture
Hi everyone What is the protocol for an acid protease test, excluding the use of hemoglobin? I am seeking cost-effective testing protocols. #acid protease #protocol
30 July 2023 4,113 3 View
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17 July 2023 8,847 3 View
Dear ResearchGate Community, I hope this message finds you all well. My name is Michael G. , and I am a Ph.D. student. We are currently synthesizing the lipid NBD-DPPE and are facing some...
13 July 2023 4,555 1 View
Pasos a seguir bien detallados de como subir los datos de RNAseq. Tipo de formato en el que subirlo Tipo de datos que subir Toda la información posible
03 July 2023 5,572 0 View
HOMO-LUMO gap on solubility
30 June 2023 8,162 1 View
Method to identify NETosis
28 June 2023 6,511 3 View
I used same dilution for negative as well as positive control
28 June 2023 9,620 0 View
Hi all, I use calcein am/ ethidium homodimer-1 kit to stain sperm. But in some cells I see both green and red signals. Should I evaluate those cells as live or dead cell? Thanks all
26 June 2023 6,199 0 View
hi, ı want to synthesis of viscous polyamine with epichlorohydrine, dimethylamine etc. for wastewater. ıt must be cheap, can be produced at 75-80 degree, finished product viscosity should be 800...
22 June 2023 5,949 0 View
I did a non-constant drug ratio combination with a fixed dose of Drug A combining it with three different doses of Drug B. When I do the Compusyn analysis it shows that the points for the CI...
20 June 2023 3,086 0 View
what is the best source of Arginine for the synthesis of polyamines?
18 June 2023 7,847 1 View
Hello everyone, I came across a question while harvesting my U2OS transfected cells. After trypsination, I resuspend my cells in cold medium with 10% FCS and I centrifuge them into four...
14 June 2023 1,686 5 View
I am currently using the TRAMPC2 cells, but I am looking for any other cell lines I can use in C57BL/6 mice.
14 June 2023 7,965 1 View
I've gotten GCMS report of my sample. But idk how to interpret that 1000 page report. My supervisor has asked me to identify glycosides in the report manually. That's just not possible with over...
13 June 2023 1,107 5 View
I wonder if I can inject Tamoxifen directly into the cisterna magna. Most of the time Tamoxifen is injected intravenously or intraperitoneally. Will there be any problem in injecting it into the...
13 June 2023 6,092 3 View
How to Production of polyamine biostimulants?
08 June 2023 9,522 0 View
What's potential risk of glias to neurons transdifferentiation?
07 June 2023 8,284 1 View
There is a small leak above the white cartridge next to the pump. Millipore does not sell a replacement cartridge, they want us to buy a whole new pump assembly! Does anyone know what kind of...
06 June 2023 743 0 View
Hi, I would like to target neuronal cells. I am looking for a suitable receptor on neuronal cells to do the same.. Any suggestions ? Do you think that DAT1 or GPR37 are suitable/good options ?...
30 May 2023 5,314 0 View
I am working on THP-1 cells. I subcultured THP-1 into 6-well plates with 3.5x10^5 cells/well. I used PMA 100ng/mL for 24h to activate THP-1 (M0), then changed the media to culture media without...
26 May 2023 8,654 0 View
I have two SH-SY5Y cell lines that stably overexpress alpha-synuclein; one the WT protein and one the A53T mutant form. I am interested to see whether I have any accumulations or aggregates of...
24 May 2023 2,438 5 View
I am after isolating neutrophils from murine bone marrow using histopaque based density gradient centrifugation. I end up with Ly6G+ neutrophils with 95% viable and 94% purity as assessed by...
19 May 2023 2,512 0 View
I will be initiating a project to study therapeutic effects of immune engagers on breast cancer. If I want to study therapeutic effects of immune engagers against breast cancer, is it better to...
16 May 2023 7,830 0 View
I am using the Lenti-X GoStix Plus kit from Takara to determine the IFUs in my viral prep from the GoStix Value they provide. However, the app is not functioning properly for me. The csv files do...
11 May 2023 3,987 1 View