Cell culture is the complex process by which cells are grown under controlled conditions, generally outside of their natural environment. | Contact experts in Cell Culture to get answers
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Questions related to Cell Culture
I found it in my cell culture. After a few times of passaging, this thing become more bigger.
25 June 2021 8,090 10 View
I am performing an assay on my adherent cells and up until the day of the experiment, the cells grow nicely and reach a good confluency of ~ 80 % cell density. However, on the day of the...
22 June 2021 883 5 View
Dear knowledgeable, Have been doing neuronal cell cultures for many years now, (yikes). I've found it useful to "balance" my media in the incubator prior to applying it to the cells. This meaning...
16 June 2021 2,862 1 View
I have a protocol that uses SDS (2%), tris-HCl (200 mM, pH 8), DTT (200 mM) and glycerol (20%) for the extraction of protein from FFPE tissue. Is there a way to understand how much...
12 June 2021 1,157 1 View
I have been frustrating with certain contamination issues for a while, and strangely I kept having the same contamination problems (yellow color and round shapes). The media was not cloudy by eye...
01 June 2021 8,518 4 View
I am trying to do a lentiviral transduction of U87 cells. I propagated viruses with PEI in HEK2923T cells. Although the protocol I used was previously optimized by our team, I failed to infect U87...
31 May 2021 9,432 2 View
Would like to ask you if you can share some protocoles explaining how to measure swelling rate What is better? Evalute it from powder or make the gel and then test it ? Thank you
19 May 2021 5,290 3 View
We are going to isolate extracellular vesicles from HL60 cell culture, but we have to culture these cell with FBS. FBS contains Extracellular vesicle which can cause interference. Do you have a...
16 May 2021 5,497 2 View
Hi, I want to analyze my qPCR (gene expression, mechanical tested and not testet) results, and have to normalize them to my reference gene GAPDH. I don't have a standard curve. I have looked at...
27 April 2021 4,750 4 View
Have no hexane available to looking for alternative methods to remove the cuticles from spinach leaves. Want to decellularize the spinach leaves to use as a scaffold in cell culture.
15 April 2021 7,452 2 View
best means a filter that mimimise the sample lost and being efficient at the same time? Any recommendations?
05 April 2021 2,895 3 View
Which valid checklist can be used to assess bias in a case report?
05 April 2021 4,556 6 View
There are few ratio for 30% acrylamide/bis solution which are 19:1, 29:1 and 37.5:1. if i prepare my stacking gel and separating gel using the recipe and ignore the ratio does it effect the gel...
31 March 2021 7,553 1 View
Hi everyone! I'm working with iPSC-derived neurons right now and differentiating my neurons using the StemCell Forebrain Differentiation and Maturation kits. I induce my NPCs using StemCell Smadi...
26 March 2021 5,269 0 View
I'm experiencing detachment of MSC monolayers during osteogenic differentiation. The cell monolayer starts to roll up after about a week of culture. I am trying to be as gentle as possible during...
15 March 2021 1,483 8 View
Dexamethasone is 100nM Glycerophosphate is 10mM Ascorbic acid is 50ug/ml. But I'm not understanding how to calculate the working concentration and mixing with media to prepare osteogenic media.
09 March 2021 8,409 4 View
I think both are used mainly for blocking GABAA receptors. Since gabazine is more selelctive for GABAA receptors, is it better to use for studying EPSC? Is it enough to block IPSC by only blocking...
07 March 2021 1,720 6 View
Hi all, Recently I tried to determine NO production in differentiated and undifferentiated THP-1 cells under several exposure conditions. I was not able to do so however, also under exposure to...
02 March 2021 5,538 5 View
I search for cell culture the medium HAM’s F10 Methionine Free...I need max 1 liter..Thanks
01 March 2021 10,161 2 View
Hello, I am wandering if anyone has a recommendation for the proper concentration of 4-hydroxytamoxifen to induce CreERT2 expression in primary pancreatic ductal organoids generated from...
27 February 2021 5,897 1 View
I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines
25 February 2021 2,436 3 View
I are working on HUVEC culture. 5 days ago, my HUVEC cell got confluent and my boss let me to continue grow it for a test later and I replaced the media in two days( day 3). Today( day 6),I found...
23 February 2021 2,118 3 View
I am trying to isolate gDNA from sorted CD4 and CD8 T cells. Human PBMCs will be thawed stained and sorted on a FACS Aria. DNA will be isolated immediately after sorting. What yield of DNA can I...
22 February 2021 1,460 1 View
Dear all, I have harvested fibroblast cells from R26p-Fucci2 mouse embryos (mouse line as described in this paper: https://dev.biologists.org/content/140/1/237) and cultured them on plastic,...
18 February 2021 3,547 2 View