Cell culture is the complex process by which cells are grown under controlled conditions, generally outside of their natural environment. | Contact experts in Cell Culture to get answers
3,711 views 5,514 posts
Questions related to Cell Culture
Hello everyone, I am encountering some issues with AFOG staining on my cryosections, and I would greatly appreciate your advice. I am working with 20 µm thick cryosections of zebrafish tissue that...
28 August 2024 5,248 0 View
Who know what is the ingredient of this substance, use in the ingredient of the White masterbatch with PP or PE Carrier. Pls kindly help me for the valuable knowledge which I will us to make the...
28 August 2024 2,561 2 View
We conducted an RCT of prepregnancy lifestyle intervention continued throughput pregnancy where one of the outcomes is fat mass. Fat mass was measured by a bio impedance analysis (using Inbody...
26 August 2024 4,716 0 View
Hello, I’m currently culturing fibroblasts, and I’ve noticed some unusual behavior. While some of the cells are growing well, others appear to have "exploded," and the media becomes cloudy....
13 August 2024 1,827 3 View
This is after completion of TFF process. Answers are appreciated. Thanks.
12 August 2024 9,471 1 View
I have done sugar analysis for several times using thin layer chromotagrahy but can't get the spots on plate for both sugar standards and samples. We use ethyl acetate-pyridine-water for mobile...
10 August 2024 9,402 3 View
ImageJ application perceives cells as background. It counts the particles in the background. How can I change this? I change it from the file invert section but it didn't work.
08 August 2024 9,588 2 View
As I have attached the over-expression profile of protein betalactamase I am working on, it seems that the over-expression is satisfactory. But through Ni-NTA chromatography the purification is...
03 August 2024 8,569 6 View
If someone knows about it, please let me know. I read the papers, but I'm confused about it.
31 July 2024 893 0 View
I wonder if I need to remove the region containing U6 promoter and free sequence to the gRNA scaffold (~1.8kb) or if this region possibly causes any side effects.
11 July 2024 8,218 2 View
During the AME-D process executed in MBfRs, gaseous substrates, CH4 and O2, are directly transferred from the membrane lumen to biofilms formed on the outer surface of the membrane, while the...
11 July 2024 7,117 0 View
I am trying to evaluate via NMR and using 13CGlucose the production of 13CG6P by Hexokinase using a preparation of fresh isolated mitochondria. The problem has been that, as they are fresh...
10 July 2024 9,364 0 View
Dear all, How to do inactivated of Saccharomyces cerisisae which has protein inside. It is uracil auxotroph. With Regards, Dipak Das
09 July 2024 3,488 3 View
Hello! I want to perform a hybrid assembly. As the genome is quite large (corresponds to a fungi) I have performed Spades and Canu assemblies (I polished this with MeDuSa and the final result is...
03 July 2024 7,921 2 View
I have encapsulated the hydrophobic drug in liposomes. I analyzed the encapsulation efficiency by HPLC for both the supernatant and the pellet. First, I centrifuged the liposomal solution, took...
26 June 2024 2,751 3 View
Writing a research proposal on medication error reporting amongst emergency nurses in a tertiary hospital in, Nairobi, Kenya, Sub-Saharan Africa.
13 June 2024 6,754 1 View
since dispersion correction GD3BJ is incompatible with minnesota, which dispersion correction should be added in calculation.
06 June 2024 8,065 1 View
Hi everyone, I wanna do proteomics of monkeys serums in 2 states, healthy and patient. How many monkeys in each group I need to run proteomics and validate my data from it? Do I deplete albumin...
05 June 2024 4,169 3 View
Hi. i have a molecule it has both amide and ester bond. When i want to get second amide bond with ester part of the molecule by an amine, but it attacks to the amide bond also. In which conditions...
02 June 2024 9,925 2 View
Below is a diagram of TEC zones on earth as a .jpeg file.
26 May 2024 4,194 0 View
To clarify the question and increase its understanding, as follows: 1. Identify educational institutions: Determine the scope of educational institutions that interest you, such as primary and...
24 May 2024 1,722 2 View
Hello I am looking for sulfo-phospho vanillin assay protocol for lipid quantification, I kindly request to provide protocol, it would be great help. Thanks
21 May 2024 6,827 0 View
Hello everyone, I’m delving into the functionalities of the Cobas Z480 analyzer and I’m eager to learn not only its basic operating principles but also about the different real-time PCR tests,...
21 May 2024 7,989 0 View
In photoautotrophic cells under light conditions, what proportion of the total ATP is generated within the chloroplasts, as a rough estimate or within a lower and upper bound, and what proportion...
15 May 2024 6,549 2 View