Cell culture is the complex process by which cells are grown under controlled conditions, generally outside of their natural environment. | Contact experts in Cell Culture to get answers
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Questions related to Cell Culture
Hi Everyone, In a phagocytosis paper I have read, it has said that "Phagocytes and prey were centrifuged (3000 × g, 1 min, including 30 s of acceleration) together prior to incubation for...
05 January 2022 4,111 2 View
Logical suggestions required with publications.
03 January 2022 2,256 3 View
I am searching for companies that provide stem cell derived exosomes from animal tissues like bovine mesenchymal stem cells, or what are the equipment needed for large scale production of exosomes...
02 January 2022 7,897 2 View
When I use RIPA buffer for protein extraction I get very low concentrations of protein. Maybe I should isolate the protein in a different buffer?
27 December 2021 5,055 3 View
I've seeded my hMSC cells in T25 flasks (in 5 ml medium), the cells will be incubated for 5 days. After 5 days, I will use the Biocolor Sircol assay kit to determine the collagen I production from...
21 December 2021 2,932 0 View
for my university project, I need a mutant Brevibacterium flavum for L-glutamine manufacturing. but I don't know which from where I can buy it. which institute sell that?
20 December 2021 177 1 View
When I culture the HEK293T cells in cell culture plates (60 or 100mm ), they grow well but when I culture the cells in 24-well cell culture plate then after one day of incubation at 37'C, the...
16 December 2021 8,339 4 View
Hi! I have a question about mycoplasma test....we use qPCR kit to check the presence of mycoplasma in cells, and hystorically we used to eliminate antibiotic from cell culture medium at least 1...
16 December 2021 5,106 3 View
Hi, Recently, I noticed that my cell flasks were doing something kind of strange. I had them stacked in groups of four. The one at the bottom seemed totally fine, but as I went up the tower, cell...
15 December 2021 587 5 View
In my experiment, I am doing sub cloning of my cells with limiting dilution technique. Growth curve is very slow. What might be the factors?
15 December 2021 5,738 1 View
I want to separate glucose, fructose, sorbitol and myo-inositol using a reverse phase isocratic system with a Shimadzu Hplc (100mm, 4.6mm, 5um). But there is a problem. Glucose, fructose and...
15 December 2021 3,059 3 View
Hello, I am culturing THP-1 cells and treating them with PMA. After harvesting them and seeding them on biomaterials, there are some "beads" appeared on them. What do you think are these? Are they...
13 December 2021 9,055 6 View
I have worked with HEK cells before and have done freezing and thawing with no problems. I am currently working with HEK 293 T cells to make lentivirus and after thawing them, I can grow them with...
12 December 2021 4,286 2 View
Dear All, At the end of a cell culture experiment, I collected the supernatant and centrifuged it, and did not notice any precipitate. After freezing the supernatant, thawing and centrifuging...
10 December 2021 6,332 3 View
Hi everyone, I'm seeding primary mouse stem cells into my PDMS-based organ on a chip device. However, the cells do not stick. We have tried coating the chip using 1: 100 and 1:20 Collagen, 2%...
10 December 2021 5,079 0 View
Hey! I have a FRET system between the inner and outer nuclear membrane, and I was wondering if I stained with DAPI or any other fluorescent antibody if that would mess up my FRET readout? As of...
07 December 2021 10,013 2 View
A bacterial strain was isolated from soil and identified as lactic acid bacteria, however, after fermentation on MRS medium for 24 hr, the clear supernatant of the fermented media was subjected...
06 December 2021 4,140 3 View
We have a field isolate of PEDv. We have been trying to propagate it in Vero cells for a year now. We have tried many types of inoculation media, ranging from DMEM supplemented with BSA and...
06 December 2021 4,924 0 View
Please contribute to this question with your assured knowledge. In treating cells for different time spans like 24h, 48h and 72h, should we seed different numbers of the cells suitable for...
05 December 2021 1,834 4 View
Can I use Molasse syrup to complete media? Do I need to add MgSO4.7H2O?
05 December 2021 7,104 3 View
Alkaline phosphatase (ALP) is an enzyme often measured as marker of activity of bone cells cultured in vitro. If the medium contains fetal bovine serum, could the ALP content of the serum affect...
02 December 2021 10,008 4 View
Hello Dear researcher Is there a way to remove the parasite spores in the hatchery center? Last year, our sub-adult broodstocks became infected with this parasite; we had to remove them all and...
30 November 2021 4,017 1 View
I am very new at culturing IPSCs. Lately, I have noticed some artifacts in my wells after the cells are incubated in mTesR Plus media for a day. Some of them are attached to the Matrigel coating...
28 November 2021 5,255 6 View
I’M trying to perform cotransfection for 293T cells in 12-well cell culture plate with two plasmids (X and Y) (DNA Conc. for X is 1016 ng/ul and 570 ng/ul for Y). I would like to use different...
28 November 2021 9,539 5 View