Cell culture is the complex process by which cells are grown under controlled conditions, generally outside of their natural environment. | Contact experts in Cell Culture to get answers
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Questions related to Cell Culture
Is sun a black body radiation and can we say that the Sun contains charges that produce electromagnetic wave?
01 December 2023 2,788 5 View
I have a primary culture of cancer cells and cancer associated fibroblasts and I am looking to enrich JUST for the fibroblast population, not the cancer cells. Does anyone have any advice on how...
01 December 2023 1,183 0 View
Do you ever have precipitation problem during preparation?
30 November 2023 3,834 2 View
Please in this type of scenario how is the discussion of my result going to be? Is it in favour of downregulation or upregulation
30 November 2023 3,339 1 View
Sunspots and solar flares has huge impacts on earth's and space weather. The live full time observation is a mandate one to predict these cycles. So curious to to know your views on it?
29 November 2023 7,760 0 View
Hi all I'm doing Sandwich ELISA , I wanted to coat the antibody to the wells , So I have many questions: 1: Coating buffer ( which is more accurate) bicarbonate -carbonate buffer: at 100mM or...
29 November 2023 4,254 0 View
As the question above, I got asked by a professor in an interview. Our trangenetic vectors (not done by me but my former superior collgues) are always this rtTA TRE. But what is the promoter of...
28 November 2023 5,140 0 View
I've used illustra Micropsin G-25 columns to purify DNA. In the user manual, however, I couldn't see any method to regenerate. So, I just put 250 uL of NFW not to make it dry. Is it possible to...
27 November 2023 6,749 0 View
I`m looking for a protocol how to grow mature/immature mouse B cells from pro-B cells in vitro. I need to grow B cells from pro B cells from extracted mouse Bone Marrow. What are the conditions...
27 November 2023 5,722 1 View
For isolation of PBMC, at what temperature we should keep sucrose solution. Which method is better for PBMC isolation? Ficoll DGC method or Sucrose DGC method?
27 November 2023 3,698 2 View
how can i form the schematic diagram of g quadruplex as shown in figure?
25 November 2023 1,810 1 View
My question is self explanatory.
25 November 2023 5,689 1 View
Do the lengths of the parallels keep on as they move toward the poles and where is the sun most concentrated?
23 November 2023 5,704 0 View
Hi, I have recently started ALI culture of Calu3 cells. Before the airlift my cells grew to a confluent monolayer with a nice TEER value, but after airlift and change to ALI maintenance media from...
22 November 2023 6,636 2 View
My cells are forming a rare clump during harvesting. I harvest at 80 to 90% confluence using 1X trypsin (0.5 g porcine trypsin and 0.2 g EDTA, 4Na per liter of Hanks' Balanced Salt Solution with...
22 November 2023 5,199 1 View
I want internally labelled RNA oligos at a reasonable price. Could any one help?
21 November 2023 7,971 0 View
Hello, I have been exposing human chondrocytes to LPS (Lipopolysaccharides from E. coli O111:B4) in concentrations from 1 µg/ml to 40 µg/ml for 24 h and 48 h and haven’t seen any decrease in cell...
17 November 2023 5,265 2 View
We are doing a lot of recombinant protein expression in our lab, and also doing phage display in a seperate room in another building. Recently the phage are eating our E. coli in protein...
17 November 2023 8,184 1 View
I need a detailed protocol of SDS-PAGE only for drosophila if anyone has done before
17 November 2023 7,752 1 View
I'm starting my research on the relationship between agriculture and technology (or agro 4.0) and I would like help from the academic community to know which factors I should pay attention to.
17 November 2023 969 4 View
Hello everyone, Can you tell me a standardized protocol for the differentiation of mesenchymal stem cells into oligodendrocytes?
15 November 2023 1,512 1 View
In the MTT assay, I read the absorbance values at 570 nm. However, sometimes there are so many deviations in the absorbance values. Hence, I have to take out some (up to 4 values) deviated values...
14 November 2023 4,889 0 View
a retina layer
13 November 2023 6,119 2 View
A polymere surface treated with plasma, as a resulte the centact angle decreased. Can we affirme that the surface roughness was increased? is there a constant relationship between surface...
13 November 2023 4,714 6 View