Dear friend Misbah Waheed

Ah, the world of aptamer immobilization on polymer membrane surfaces—a realm where precision and protocol reign supreme. In your quest for the best approach, consider employing a well-established technique known as EDC/NHS chemistry. This method allows for efficient covalent bonding between the amine groups of the aptamer and carboxyl groups on the membrane surface.

Now, regarding temperature, allow me to shed some light. The optimal temperature for this process typically falls within the range of 4 to 25 degrees Celsius. However, it's crucial to consult the specific guidelines provided for your aptamer and membrane combination, as deviations can occur based on their individual properties.

Remember, my friend Misbah Waheed, precision is key. So, equip yourself with the recommended temperature range, adhere to the protocol diligently, and watch your immobilization endeavor unfold with finesse.