Cell culture is the complex process by which cells are grown under controlled conditions, generally outside of their natural environment. | Contact experts in Cell Culture to get answers
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Questions related to Cell Culture
I have liters of cell lysate and I need to separate the recombinant protein from the rest. The volume is very large for the affinity chromatography column. Any other technique? I don't need high...
29 September 2023 4,173 4 View
I have prepared DNA libraries; the fragment size is about 300 to 700 bp. In the sequencing results, I see the Mean insert size is 42bp.? The sequencing platform used was Novaseq PE150. DNA...
29 September 2023 9,354 1 View
There are limited research about Entada phaseoloides L. which is hard to know whether what extraction process of its stem bark. I saw few studies but they are reviews or an article
27 September 2023 3,035 0 View
What is the concentration of the Magic Red® Cathepsin-B Assay Kit, 25 test ?
26 September 2023 8,028 1 View
Hello community, I am kindly asking for your help. I was wondering if someone knows/or has used a protocol for extraction of amino acids and antioxidant amino acids from microbial culture...
25 September 2023 5,298 1 View
I am performing a meta analysis in which there are some studies which are RCTs and cohorts. There are two studies which are non-randomized controlled trials. Can I include those two studies in my...
24 September 2023 461 3 View
I have been culturing HUVEC and sometimes the cells look stressed and stop dividing. When this happens, they don't recover after I change the medium. What may be causing this problem? Expired...
24 September 2023 2,552 3 View
I am running a neutral comet assay on HS-5 cells and I am having a rather difficult time producing comets. My thought is that the cells aren’t lysing properly. The only time I’ve seen this assay...
23 September 2023 7,205 0 View
I have a colony formation assay. I am seeding cells in a 96-well plate. The cell line is NTERA, 150 cells per well. I am seeding the cells with DMEM fortified with 10% FBS. Each well will have...
20 September 2023 9,082 2 View
I will use retinoic acid (Sigma/R2625) and as I know is sensitive to air and light. After dissolving the chemical, I want to store my aliquots in an inert environment, but I do not have suitable...
19 September 2023 6,674 3 View
Dear all: My python version is 2.5 and autodock is 1.5.7, but my MGLtool folder shows up in python 2.7.11. I had opened the software before, but then there were some problems when it ran, so I...
18 September 2023 1,239 1 View
how can i evaporate ethanol from extract without rotary evaporator?
18 September 2023 2,213 2 View
Hi all! I have done the LDH cytotoxicity assay on A549 cell line recently. I seeded 104 cells / well in 96 well plate. Then I used a lysis buffer in ELabScience LDH Cytotoxicity kit as the...
16 September 2023 4,113 0 View
Does anyone have a protocol for measuring T lymphocyte activation, either by flow cytometry or by measuring cytokine production? I'm working with CAR-T lymphocytes and I want to see if incubating...
14 September 2023 1,480 2 View
Why is it that when I search for literature on 3D printing bone scaffolds using materials like PLA, I seem to find more studies using FDM rather than SLA? And I have another related question: I...
12 September 2023 5,615 3 View
I am trying to grow some mouse derived tumor cells (benign tumor with just one flox/flox gene recombination) (identified by GFP expression due to Cre recombination - model well defined) in sphere...
11 September 2023 3,525 0 View
The in situ treatment of mercury contaminated soils by injection of an inorganic reactive gel. Thank you in advance for your responsiveness.
11 September 2023 9,571 2 View
I want to knock out a non-essential gene in endometrial cancer cell lines and am wondering what is the most straightforward approach to do this efficiently. Which is the best CRISPR system to use?...
08 September 2023 203 4 View
Hello everyone! I need to perform a MTT cell viability assay, but I am not sure which kit would work best with our lab's plate reader. Below is an image of the absorbances that our machine reads:
08 September 2023 6,885 1 View
How to identify the correct size of a large DNA band (>20kb) after being digested by a unique restriction enzyme? I have recently digested a large plasmid with a size of 20,208 bp by XhoI, a...
08 September 2023 8,903 4 View
I've been using Aspen Plus software to simulate sludge gasification and far I've relied on the information and explanations provided in literature to specify the equipment. However, I'm currently...
06 September 2023 1,167 3 View
We received a stock of contaminated vials. After discovering contamination in the fifth thawed vial, we must assume the entire stock is affected (12 vials). This issue appears to be isolated to...
05 September 2023 9,405 16 View
Dear All, I want to know if is there anyone that ever used LDH Cytotoxicity Colorimetric Assay Kit from ElabScience, cat number: e-bc-k771-m? I did an experiment and I had some strange results,...
05 September 2023 3,543 2 View
Hi, I am looking to try a cross-linking protocol to assess alpha-synuclein species in my adherent cells. I have DSS for this and have found several papers with a semi-descriptive method but I...
04 September 2023 3,706 0 View