Cell culture is the complex process by which cells are grown under controlled conditions, generally outside of their natural environment. | Contact experts in Cell Culture to get answers
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Questions related to Cell Culture
I am doing primary fibroblast cell culture and transduced my cells with a lentivirus with 10^7 TU/mL. I added 7.5 uL of virus to about 250,000 cells to have an MOI of about .3. Of note polybrene...
08 September 2021 7,019 3 View
I am doing cell culture of THP-1 cell line, at the first culturing and before incubation, 90% of the cells were dead because they were not stored in liquid nitrogen but in -80 C. How can I...
06 September 2021 1,551 4 View
I'm having an issue with differentiaon media (DM) of MC3T3-E1 Subclone 4 cells I am following the ATCC recommendations for media and supplements (alpha-MEM + 10% FBS + 1X anti/anti...
06 September 2021 5,928 5 View
While evaluating the cytotoxicity of a sample in cell line, what percentage of cell viability is considered inorder to conclude that the sample is toxic. Like if 80% of cell survives, is the...
05 September 2021 8,861 4 View
Hello everyone, I need genomic E. coli DNA as a template for later PCR. I have a protocol how to isolate genomic DNA and it says I have to prepare an overnight culture of XL1 blue cells. We don't...
02 September 2021 8,895 3 View
I want to use an ELISA kit based on competitive evaluation, and in the documentation of the kit they mention "influenced by the factors including cell viability, cell number or sampling time,...
01 September 2021 4,636 3 View
I am now working on cell culture bioreator characterization, inlcuding mass transfer (O2 and CO2) and mixing studies. Once I have the volumetric mass transfer coefficinet (kLa) data, I want to...
31 August 2021 9,387 1 View
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30 August 2021 6,127 6 View
Hello I am currently trying to amplify helper phage VCSM13 with TG1 E.coli. The problem is that I have tried very hard to amplify several times but it all failed, ending with no plaques. (LB agar...
29 August 2021 8,719 7 View
Request cell culture medium composition for mouse primary hepatocytes to study HDL uptake
28 August 2021 5,119 2 View
I am looking for a 11BHSD1 specific inhibitor to use in a rat cell culture
27 August 2021 7,429 1 View
Hi everyone, I have found a few articles that give recipes for making cell culture media specifically for human neutrophils. I would like to do experiments where I would procure and magnetically...
26 August 2021 7,819 1 View
I'm working with H9C2 cells for the first time and I've been following protocols I could find on papers and research gate. For the sub-culture, I seed 10k cells/cm2 and split the cells every 2-3...
23 August 2021 1,881 3 View
I have noticed morphological changes in primary melanoma cell cultures after switching to a different brand of FBS, the cells are circled in blue. Some cells began to form a more round shape with...
20 August 2021 9,021 3 View
I'm aware of: Panexin and FastGro Synthetic, Animal Free, Chemically Defined FBS Replacement. I'd like to supplement my standard basal media, if possible.
18 August 2021 2,156 1 View
How to isolate machrophages from 96-well plate? I don,t want to use cell scraper and trypsin solution and I don,t have a rubber policeman for isolation of cells. so whats an easy and safe way to...
16 August 2021 8,573 5 View
I am trying to isolate neural stem cells from the spinal cord of 6 month old mice. I'm washing the sample 4 times with 1X PBS + 2% P/S and 1% Amphotericin. Dissociating into small pieces and then...
16 August 2021 2,351 2 View
I want to culture renal proximal tubule epithelial (RPTC) cells on a transparent membrane for microfluidic systems.
13 August 2021 9,710 1 View
I am doing a cell culturing experiment in a fluid flow chamber made of PDMS material. I was wondering how to sterilize the pipes and the flow chamber before/after the experiment, in case I want to...
11 August 2021 5,229 3 View
My protein having his tag which I have purified using Ni-NTA chromatography. I want to use purified protein for Cell culture studies on Macrophages. To study entry of protein into the cell, I want...
11 August 2021 6,844 3 View
I have been culturing lymphoblastoid cells and have been observing these small, black particles for over a month now. The particles are smaller than the LCL single cells, they do not move but...
06 August 2021 3,927 2 View
Hi, I'm planning to quantify cells with different receptor expressions in stem cells throughout the 21 cell culture phase. Can I fix cells in 4% formaldehyde on selected days and conduct the flow...
06 August 2021 7,053 3 View
We are growing amniotic mesenchymal stromal cells in culture. The cells are isolated from a term placenta, delivered via C-section, using collagenase. We culture the cells in 10 % FBS DMEM (0,1 %...
06 August 2021 5,786 4 View
Initially there was a flask at P13 which was growing very fast using all ATCC recommended conditions and products (EMEM + 20% FBS). I then switched to using EMEM from Quality Biological, and used...
05 August 2021 6,829 3 View