How to calculate the crystallinity of cellulose film?

27 February 2021 2,753 4 View

How does GROMACS calculate the average structure of proteins?

I want to get a average structure from a pdb file containing multiple models. I used the command below: gmx cluster -f input.pdb -s topol.tpr -cutoff 20 -method gromos -av GROMACS...

25 February 2021 6,314 1 View

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The regenerated cellulose film was prepared by zinc chloride/H2O system. The zinc chloride had been washed away in the regeneration process, and the wet film which was not dried had uniform...

18 February 2021 9,538 1 View

R/Python package for time-intensity or progressive profiling in sensory evaluation?

Hi I've been looking for a stat package for my sensory evaluation data (progressive profiled which is a multi-timepoint version of descriptive analysis). Is there any available one to...

08 February 2021 9,170 3 View

How can I make sure the simulation results of the water droplet impacting on the wall using the FLUENT agree with the experimental results?

Dear researchers, I am modeling the process of the water droplet impacting on the wall with a constant contact angle. The specific calculation parameters are as follows: The droplet diameter is...

24 January 2021 1,020 5 View

Mysterious line across SDS gel?

Does anyone have the similar issue? A line across the denature SDS gel shows up after staining with Coomassie blue and there are many lines in every lane if you take a closer look. This gel had 15...

07 January 2021 5,610 4 View

How can I calculate packing density of particles by fomulas or softwares?

I have all data of particles, such as size distribution, or numbers. Is there any way that I can calculate the packing density of these particles? Assume that all particles are circular.

05 January 2021 4,272 2 View

After cellulose is regenerated in water, a cellulose hydrogel is formed. I want to know if there is crystallinity inside the the hydrogel?

After cellulose is dissolved and regenerated in water, a regenerated cellulose hydrogel is formed. I want to know if there is crystallinity inside the hydrogel, or it will not crystallize until it...

04 January 2021 7,466 4 View

How can I fix the density of system in coarsed grain simulation?

In the process of obtaining the nonbond potential of the Coarse-grained(CG) model, I need to simulate the CG model mapped from the conformation of all atom(AA). AA simulation process is...

02 January 2021 3,643 1 View

How to export Mass and Stiffness matrices using Ansys Workbench?

Hello, I am trying to export Mass and Stiffness matrices using Ansys Workbench via Modal analysis modular, with APDL commands inserted. However, there is no .matrix file generated. Could anyone...

17 December 2020 1,196 5 View

Which is the energy (J/cm^2) provided by a UV lamp (220V 50Hz) during 50 min of exposure at 5 cm distance of samples?

I am planning to do a phototoxicity experiment in which I need to apply an UV Energy lower than the highest non citotoxic energy. Based on literature it should be around 5J/cm^2 for my cell line...

04 May 2019 3,818 5 View

Are those precipitates from neutral red solution used for NRU assay?

I'm trying to optimize the NRU assay on 3T3 cells as low OD540 reading (about 0.1-0.2) were obtained from previously done experiments. I prepared 50ug/ml stock and incubated it at 37C for 1.5 hr,...

10 November 2018 695 2 View

Anone facing phototoxicity in IF samples?

Hi, Iam doing IF in HeLa cells and facing a weird problem. I use the standard protocol and when I expose the cell to laser light in the 488 channel, all the fluorescence fades the instant its...

29 September 2018 2,510 3 View

Anone facing phototoxicity in IF samples?

Hi, Iam doing IF in HeLa cells and facing a weird problem. I use the standard protocol and when I expose the cell to laser light in the 488 channel, all the fluorescence fades the instant its...

08 September 2018 6,532 4 View

For multiphoton imaging below the surface of a tissue sample, how does one determine the laser power being delivered to the sample?

06 July 2017 5,099 4 View

Where can I conduct research in photodynamic therapy and/or drug phototoxicity?

I am looking for access to laboratory in which I could conduct research in photodynamic therapy and/or drug phototoxicity. I would like to continue my research in molecular and cellular...

02 March 2014 2,202 2 View

Can someone advise on why is there precipitation when the Neutral Red in the In Vitro 3T3 NRU Assay? And on how to get rid of the precipitation (in the form of crystals)?

Precipitation (crystals) formed in the Neutral Red Assay

06 July 2013 5,286 6 View

Calculation of invitro photo tox

Can anyone share the excel for calculating mean photo effect in invitro phototox studies. Regards, Dr.V. Magesh

01 January 1970 114 0 View