Dear all,
I used the the software "Phototox Version 2.0" to calculate PIF and MPE. In a dose-effect figure generated by the software contain some data, see attachments for details.
Does anyone know what they mean?
Looking forward to your reply!
As described in the Multifractal detrended fluctuation analysis (MFDFA) algorithm, it at first calculates the profile of the time series, and then other steps are operated on the profile....
05 August 2024 9,366 2 View
Hi, Could someone explain the primary differences between deep-seated landslides and slope destabilization? In particular, definition and characteristics, mechanisms and triggering factors,...
02 August 2024 4,212 2 View
How long it takes for arbuscular mycorrhiza to establish and produce benefits under experimental conditions?
25 July 2024 5,208 2 View
I want to study the thermal properties of a mixed system which is constructed by virtual crystal approximation in VASP. When I try to run the ab initio Molecular Dynamics of this system in VASP, I...
19 July 2024 6,569 3 View
If I want to invent my own hypothesis testing method, where should I get started ?
15 July 2024 5,376 5 View
I am currently working on a research paper focused on the control of Connected and Autonomous Vehicles (CAVs) utilizing multi-agent reinforcement learning methods. At this stage, I am seeking a...
14 July 2024 2,620 2 View
I've earned 1 best paper award and 4 best oral presentation awards. What should I do next to elevate my academic capabilities to the next level ?
14 July 2024 6,071 5 View
I read several information security books. How do I start writing anti-virus softwares ?
13 July 2024 8,180 1 View
I suspect my cells are contaminated with mycoplasma. I fixed the cells with 4% PFA and stained them with DAPI. Below is the image I obtained. I don't observe the typical small, rounded DAPI foci...
11 July 2024 7,786 3 View
During the heavy warm rolling process, austenite undergoes dynamic recrystallization (either DDRX or CDRX), and then transforms into martensite upon quenching. Is there a way to distinguish from...
11 July 2024 7,602 1 View
Dear reader, In the course of my experiments in accordance with the OECD Guideline TG No. 432, the OD value after irradiation is always only about 80% compared with the OD value of the...
10 July 2023 3,046 0 View
Hello, I am currently working on the 3T3 NRU phototoxicity test (OECD 432). However, during the 1hr 50 min incubation in EBSS buffer the cells are detaching from the microplate. Has anyone else...
16 November 2021 4,555 1 View
Dear readers, I am a master degree student currently working on phototoxicity assessment using Balb/c 3T3 cell line. However, I have encountered some technical issue during the running of this...
15 July 2021 5,113 6 View
I am planning to do a phototoxicity experiment in which I need to apply an UV Energy lower than the highest non citotoxic energy. Based on literature it should be around 5J/cm^2 for my cell line...
04 May 2019 3,894 5 View
I'm trying to optimize the NRU assay on 3T3 cells as low OD540 reading (about 0.1-0.2) were obtained from previously done experiments. I prepared 50ug/ml stock and incubated it at 37C for 1.5 hr,...
10 November 2018 816 2 View
Hi, Iam doing IF in HeLa cells and facing a weird problem. I use the standard protocol and when I expose the cell to laser light in the 488 channel, all the fluorescence fades the instant its...
29 September 2018 2,678 3 View
08 September 2018 6,665 4 View
I"m using a Nikon A1R multiphoton system to image GFP-labeled cells below the brain surface, such as in cortical layer II/III (100+ µm deep). The system indicates the power output at a given...
06 July 2017 5,132 4 View
I am looking for access to laboratory in which I could conduct research in photodynamic therapy and/or drug phototoxicity. I would like to continue my research in molecular and cellular...
02 March 2014 2,390 2 View
Precipitation (crystals) formed in the Neutral Red Assay
06 July 2013 5,406 6 View