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Questions related to Cytological Techniques
I am humbly asking for some advise or tips to do this kind of methods for experimenting a new staining agent from plants. And this experimented staining agent is used for the light microscopy only.
06 June 2016 5,446 0 View
Please describe the methods and techniques to prepare the slide, staining requirements and study the cells of plant leaf affected by pollutants.
06 June 2016 5,637 4 View
I'm using this protocol, but with charged slides: http://www.aatbio.com/products/protocol/23062.pdf The sections are from mice that were perfused with 4% PFA and post-fixed in 4% PFA overnight,...
06 June 2016 9,055 3 View
Could someone send me a good protocol to evaluate markers from mesenchymal stem cells by flow cytometry? if we have to fix the cells before the protocol or not, incubate with fbs or if we have an...
05 May 2016 473 6 View
Can I thaw the freezed tissues such as endometrium in liquid N2 and place it in formalin solution for using in immunohistochemistry?
05 May 2016 8,561 3 View
i am planning to study the effect of local honey in chemotherapy induced mucositis specifically in tongue in rat model. for this i have to excise the tongue and make the section. so my question is...
08 April 2016 7,054 3 View
04 April 2016 7,841 4 View
Those are the 3rd passage of human bone marrow MSC cell lines. The original cells ordered from company. I searched that frozen those cells under liquid nirtogen would decrease their...
03 March 2016 8,569 1 View
I have estimated Nitric Oxide from tissue homogenate by using griess reagent. From the standard curve, I got a concentration about 12 µM. I have used 100 µl of tissue homogenate. The protein...
29 January 2016 4,807 4 View
01 January 2016 7,557 5 View
I'm using H&E.
11 November 2015 7,002 4 View
During heat antigen retrial wash out of smear. how can we manage it?
10 October 2015 8,331 6 View
For fluorescent in situ hybridisation (FISH), how can we fix bacterial samples (both gram +ve and -ve) using ethanol? As I can't use paraformaldehyde (PFA) fixed samples for my experiment.
08 August 2015 6,424 7 View
We usually fix the brush with alcoholic solution, and than do a cell clot. But we have crush artifacts that don't allow the cytological evaluation. Can you suggest me something to improve this...
08 August 2015 1,621 3 View
This question pertains to urinary cytology specimens when screening for high grade urothelial carcinoma.
29 July 2015 4,866 2 View
07 July 2015 6,056 2 View
I am specifically working with human brain tissue. Most of the tissue has been in formalin for 2-10 years. I have read a couple papers claiming to have revived their tissue with certain...
27 May 2015 2,154 4 View
05 May 2015 9,861 5 View
what procedure should I follow to stain rice (Oryza sativa) chromosomes for cytological studies?
05 May 2015 6,917 2 View
I have been differentiating K562 cells and would like to count the differentiated cells using a haemocytometer. I have tried SIGMAFAST DAB as well as o-diansidine.
04 March 2015 4,601 5 View
03 March 2015 4,133 6 View
I´m currently working with primary glioma cells cultured in DMEM suppl. with 15% FCS grown adherently and I´m trying to establish a ROS staining with DCFDA (from santa cruz). As I would like to...
26 November 2014 4,512 6 View
I want to know the distribution of Mreg in mouse tissue, which marker should I choose?
11 November 2014 1,202 1 View
11 November 2014 9,491 7 View