Cell culture is the complex process by which cells are grown under controlled conditions, generally outside of their natural environment. | Contact experts in Cell Culture to get answers
2,128 views 4,206 posts
Questions related to Cell Culture
I would like to detect mycoplasma contamination in cell cultures. Could someone provide me relevant protocols and references? Thanks.
20 October 2021 2,490 3 View
Given the cost and delay of plasticware, I'm wondering how many have had success coating their own plates? Specifically, I'm interested in protocols for primary trout hepatocytes on collagen...
19 October 2021 9,275 2 View
I currently try to evolve a cell culture model with MSCs from tracheal aspirate of prematures. I would like to simulate an infection with gram-negative and gram-positive bacteria in cell culture....
15 October 2021 3,088 1 View
hello guys, two days ago I plated the cell in 10 Petri dishes the confluence was not too much so I waited for them to reach 100% in order to split them today unfortunately when I check them today...
15 October 2021 6,867 13 View
I was about to harvest my 2.5L cell cultures and noticed tiny spheres at the bottom of the 5L flask. They looked like tiny dots that were white. Under the microscope they didn't seem to be any...
14 October 2021 8,805 3 View
Hi All, Can anyone help us identify the contamination we have been seeing in our Jurkat cell culture. It appears as small spherical structures which suddenly appear after the first few passages...
14 October 2021 9,036 2 View
I want to know what is the required or the enough period to check the cytocompatibility of the decellularized biomaterials to the seeded stem cells
13 October 2021 2,676 3 View
hi all, I have a hybridoma cell which is described as suspension cell. This is the first time I do suspension cell culture so I would like to ask: 1. Do all the suspension cells need agitation...
12 October 2021 8,637 0 View
Hello everyone, I’m start working on HEK293 cel culture and They have very slow growth rate. today is the the third day of cell culture even though the media DMEM contain 10% FBS penstrept and...
08 October 2021 8,706 3 View
Matrigel is at the lower and upper of cells. I want to isolate cells from the plate and matrigel and use them for WB. I used Cardiomyocyte Dissociation Kit Catalog # 05025 but It solved the matrigel.
08 October 2021 2,391 1 View
I would like to be able to differentiate THP-1 into macrophages of several types, in particular M1 and M2a. To do this, I activated THP-1 cells with 200 ng/mL PMA for 72h, than I differentiated...
07 October 2021 130 4 View
Kindly explain what kind of contamination is seen in the attached files. This is SKOV3 cell line. To remove the contamination we have treated it with Gentamycin but couldn't get rid of it.
06 October 2021 7,062 3 View
hi everyone, I work with neuronal cells. i split my last flask and left my new flasks up side down. i.e. i flipped them. the cells have attached to the surface ( which was supposed to be the up...
04 October 2021 7,961 3 View
UPDATE: the issue was the flow pressure being too high and cells being too sensitive to a loss of membrane integrity. I lowered the flow pressure and used a reagent/buffer that is designed to...
01 October 2021 1,344 2 View
I've seen to use betaine in cell culture media as osmolytes. We have in the lab betaine hydrochloride but I don't know if it's preferable to use glycine betaine in this case. Does anyone use...
01 October 2021 3,909 2 View
Is this contamination or cell clumping? its a flask of hek 293 cells 24hrs after passaging. no clour change or turbidity of media.
29 September 2021 5,933 5 View
I have suspicious of contamination in my cell culture flasks but I am not sure. at the same time, I need to check my medium whether is contaminated with bacteria or not. so what is the most...
29 September 2021 1,915 2 View
I see that many papers include analyzing calcium in their methods when the differentiation of iPSCs to neurons is taking place. Why is this necessary?
28 September 2021 7,217 2 View
Hello, I'm looking for a protocol that I can use/adapt for the purpose of scaffolding used in cultivated Meat. Any tips, Thanks Jordan
26 September 2021 3,600 0 View
Hello all, I'm trying to isolate and expand human primary renal fibroblasts from nephrectomy samples using an explantation technique. This technique has already been used successfully in my lab...
20 September 2021 4,761 5 View
Hello, I want to prepare a 3D skin model in transwell. What parameters should I pay attention to?What parameters should I pay attention to? Pore size, type of membrane (PET or PS), membrane should...
20 September 2021 4,615 0 View
Hi folks, I want to eliminate a specific protein from cell culture supernatant, any ideas please? Appreciate if anyone can find a paper of this method. Many thanks.
13 September 2021 10,003 2 View
I would like to use flow cytometry to characterize the cells of a Single Cell solution of human skin samples. Does anyone have a tip which marker is suitable for keratinocytes? Thanks!!
13 September 2021 5,109 2 View
We have to make a large scale cell culture of suspension cells and we were wondering if we can use regular petri dishes instead of coated cell culture dishes. Actually I have tested this before...
11 September 2021 5,442 4 View