Cell culture is the complex process by which cells are grown under controlled conditions, generally outside of their natural environment. | Contact experts in Cell Culture to get answers
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Questions related to Cell Culture
The following text explains quantum entanglement and its limitations in exchanging information. Quantum entanglement refers to a correlation between the states of particles that are separated by...
20 March 2024 9,293 4 View
Specify absorber conditions and specifications and if possible flow rates of both the feed gas and solvent.
19 March 2024 9,539 0 View
For example, If a PCR primer can detect ~100 fg of DNA, how we will calculate it to Bacterial copy number?
18 March 2024 1,897 2 View
how to isolate stem cells from bone marrow?
15 March 2024 2,471 1 View
The journal should have the following features - 1) It must have score of 5 or more in the list of NAAS rated Journal (effective from 1st January, 2024). Journals with Impact factor (Thomson &...
12 March 2024 3,452 4 View
I am new to using MTT assay. My experiment is to treat cells and see the cell viability after 24,48,72 hrs so should I grow cells in three different plates and put MTT reagent for each one...
10 March 2024 6,550 4 View
Greetings of the day, Dear Friends, I hope you are doing well. Kindly discuss the general pipeline/methodology/steps to do Next-generation sequencing (NGS). Can Anyone tell me where i can learn...
08 March 2024 611 4 View
I want to see adipocytes from this material in slides with oil red. I can decalcify and place in OCT by making cuts on silanized slides? How thick is the cut? Can my cryostat cut decalcified...
08 March 2024 1,804 0 View
Hi all, I used Merk panels for luminex200 analysis on CSF, but of the same analytes I now don't have the corresponding validated panels on serum. Therefore I was wondering if possible to redo...
06 March 2024 9,112 1 View
Citations in research gate need to re-evaluate .
06 March 2024 5,984 8 View
I am currently working on HeLa cells and looking forward to conduct experiments but I was wondering what would be the ideal HeLa cells density/concentration per surface to conduct experiments?
27 February 2024 6,378 2 View
Dear all, did any of you already try to obtain sarcospheres from a continuous cell line of a canine osteosarcoma ? Do you have some nice protocol to suggest (if it is published we are glad to cite...
26 February 2024 4,634 0 View
I am working HIV. My RNA concentration is 57-65 ng/ul. AFTER cDNA preparation I did not get any PCR result. Only dimer are on the gel. Highly Visible dimers are on gel.
21 February 2024 9,130 3 View
In the suspension polymerization, the monomer connects to form a linear chain, then how and why the linear chain coils to produce round particles?
21 February 2024 3,508 3 View
glycogen in serum
20 February 2024 1,634 1 View
How can I seed 0.5x10^6 cells per well in a 96-well plate (I only need to seed 5 wells)? I have a T25 flask (5 mL total volume) with a cell density of 0.6x10^6 cells/mL.
18 February 2024 5,597 1 View
Please see the file attached to this message. If I click on "View request". there is no option to decline or to respond to requests, just a reference to the article or chapter itself. Please help.
14 February 2024 2,012 0 View
Hi all, We seek an efficient electroporation protocol for patient-derived preadipocytes and SGBS cells to perform co-transfection for CRISPR/Cas9. They are pretty sensitive and hard to transfect....
13 February 2024 4,905 0 View
Preprint papers and publications allow researchers to share their findings to the research community to get critiques, comments that will help improve the quality of the paper before submitting...
12 February 2024 3,879 2 View
Hi "I am conducting transcriptome analysis on a parasitic plant for which no whole genome is available. I would appreciate assistance in suggesting steps for analysis after Trinity assembly,...
06 February 2024 6,490 1 View
A primary difference between photons would be: Absorption/emission events, Hz, and eV associated with sunlight photons Optical photons mostly travel in uG vacuum with less gas RF Photons travel...
05 February 2024 5,667 4 View
hello everyone! HeLa cells were seeded in 6-well plates at 200,000 cells per well. They were left for 2–3 days to reach 70–80% confluency. Then, determination of protein concentration was...
01 February 2024 2,593 5 View
Are there any conditions where it would be possible to lyse gram-negative bacteria using the Promega PLB? I would need to lyse the cells to test for crude enzyme activity but I don’t have a...
31 January 2024 7,673 1 View
I wanted to convert few 2D compounds to 3D using Avogadro and then use them for docking. Are these ligands efficient for docking? Will I get accurate results. Thank you in advance.
30 January 2024 9,846 2 View