Cell culture is the complex process by which cells are grown under controlled conditions, generally outside of their natural environment. | Contact experts in Cell Culture to get answers
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Questions related to Cell Culture
tldr: I have a dry well of a 24 well plate that did have suspension CHO-K1 cells in it up until about 5 days ago when I upscaled them. The plate has been kept in a humidified incubator since (a...
04 August 2021 411 3 View
I have been routinely using TG1 for phage display pannings but am having difficulty obtaining high quality sequence data from clones sequences from phagemid vectors in TG1, likely due to EndA. Is...
04 August 2021 9,213 0 View
Good morning to everyone, To induce my cells, I use a medium (DMEM, 4.5 g/mL glucose) supplemented with vitamin D, vitamin C and Beta- glycerophosphate. I used this mix for long, so I know it...
03 August 2021 6,130 3 View
There's something floating in my medium, it looks strange. but I don't know what is it. It didn't cause pH change and the medium is still clear, also the cells grow well. (the cells haven't been...
03 August 2021 2,723 3 View
I am interested in the isolation of chicken primordial germ cells from embryo blood and I am following Yamamoto et al (2007) A Novel Method to Isolate Primordial Germ Cells and Its Use ... . Here...
03 August 2021 9,318 3 View
I am observing black dots (as you can see in the empty spaces where there are no cells) in my cell culture as attached in the picture. I think they are motile. The media has not become turbid or...
03 August 2021 6,961 7 View
I am working on HEK293T cells and trying to make gene specific knockout cell line by using Crispr Cas9, but at the end of single clones the success rate is very low.
28 July 2021 7,456 3 View
My lab is now far past when we should have microglia in our 24-well cultures. We have plenty of what we assume to be astrocytes lining the bottoms of the entire wells but we cannot seem to get...
27 July 2021 8,952 2 View
I am currently culturing some HEK cells with absolutely no issue, they are being cultured with the standard DMEM + 10% FBS media. For a particular experiment, I need to introduce exogenous...
22 July 2021 4,202 3 View
Hello everyone, I need some high glucose DPBS for temporary cell culture. I decide to make it from DPBS without glucose. Can I directly add glucose to DPBS without glucose? Since I think adding...
19 July 2021 9,615 3 View
Hi guys, After 72 hours of transduction of Adeno to Hec cells the color of the medium change to purple, cells have been de-attached and many tiny particles are seen. Have you had any experience...
17 July 2021 3,376 1 View
Dear all, I need to culture whole blood in a 37°C incubator for 24h prior to RBC lysis and analysis. If blood is sampled in Na-Heparin tubes, i think that it is required to add additional Na...
15 July 2021 3,693 4 View
I am treating my cells with a drug that generates about 20-40 uM H2O2. I see a profound activation of gamma-H2AX but no/very low levels of corresponding DNA damage in comet assay. I am trying to...
15 July 2021 6,033 4 View
I cultivate keratinocytes isolated from the foreskin. Initially, after the isolation of keratinocytes, only single fibroblasts are visible, but after two passages, the fibroblasts cover most of...
14 July 2021 5,530 2 View
I was asking myself why we usually change the media just the day after thawing the cells. It seems that the two reasons most of the people exposed are: 1. To get rid of the DMSO leftovers. 2. To...
12 July 2021 9,715 4 View
Hi everyone, I have recently thawed HL60 cells that had traveled in dry ice overseas and did not melt during the travel. When I first opened them they seemed to be okay and proliferating. After a...
09 July 2021 4,578 2 View
I'm working with MDCK cells, but unfortunately there's a contamination appear on my cell culture with the some characteristics as follow; 1) the colour of the medium is clear yellow, not turbid;...
08 July 2021 9,439 7 View
We work with lymphoblastoid cell lines derived from EBV transformed PBMCs. Recently, one of the lines I have been culturing has ceased growth, despite forming healthy-looking clumps. I have...
06 July 2021 4,430 3 View
Hi all, I am working on EdU prolifetration assay with flow cytometry. I followed the manufacturer's protocol, however I had a very high signal in negative staining control (with Click-iT but...
06 July 2021 5,092 3 View
I have been having difficulty differentiating C2C12 cells into myotubes. I use DMEM media with high glucose + glutamine + sodium pyruvate supplemented with 2% horse serum and 1%...
05 July 2021 7,074 5 View
I initiated LCLs 8 weeks ago with 1ml EBV/flask and RPMI with 20%FBS. We are facing the following problems. 1.The cells are small in size ,not expanding as frequently as suggested by established...
03 July 2021 4,365 2 View
Hello, I will develop materials for the repair of nerve damages in my master thesis, and I am planning to use neural cells for cell culture experiments. I have cell culture knowledge, but I have...
02 July 2021 5,511 4 View
Hello everyone, I've done a few ELISA tests and I'm wondering about how to mix correctly my samples (cell culture supernatant) and the dilution buffer during an ELISA experiment. For some...
01 July 2021 769 4 View
Can we use DMEM high glucose (4.5g/L)/D-PBSA mixed (3:1, final volume 0.06mM for Low Ca) without FCS for grow mouse keratinocyte cells? We have not supplements for basal medium.
01 July 2021 8,361 0 View