Cell culture is the complex process by which cells are grown under controlled conditions, generally outside of their natural environment. | Contact experts in Cell Culture to get answers
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Questions related to Cell Culture
I am reaching out for advice on some challenges we have faced with inducing pluripotent stem cells (iPSCs) to differentiate into mesenchymal stem cells (MSCs). Plan 1: We choose P30 generation...
01 September 2023 1,282 0 View
The differentiation of arthropods spans an exceptionally long period of evolutionary history, surpassing that of many vertebrates. Consequently, the process of ancestral chromosome reconstruction...
01 September 2023 8,173 0 View
Actually I am having issue with double purified PCR bands for gel electrophoresis..
30 August 2023 7,685 5 View
GC-MS analysis of Bacteria
30 August 2023 2,322 1 View
Hi all. I am attempting to transfect a ~10kb plasmid into MJ cells using LP3000. MJs are a lymphocyte cell line that is chronically infected with HTLV-1. Downstream, will transfect siRNAs using...
30 August 2023 2,323 0 View
I want to measure the root volume of ramie plants grown in pots. I have replicated the trial, and I want to calculate the root volume of each plant in each replication. The procedure is unclear,...
29 August 2023 6,525 2 View
As we all know, stimulating T cell with PMA in vitro can effectively activate T cell unspecificly. If we want to measure T cell activity in tumor, will stimulating in vitro make it too artificial?
24 August 2023 9,649 0 View
any expert suggestion?
23 August 2023 6,185 9 View
I WANT TO KNOW ABOUT AGV
22 August 2023 2,265 0 View
For instance, I am working on thirsty brain samples for immunoflouresce technique and imaging using laser confocal microscope.. how well would you advise, this is planned on a daily basis?
21 August 2023 355 0 View
please answer.
19 August 2023 1,953 7 View
The role played by every peptide involved in neuroexocytosis
18 August 2023 6,674 2 View
please share an easy-to-follow protocol of lowry assay, along with the method to prepare BSA standard graph in the range of 5ug/ ml to 100ug/ml
17 August 2023 8,529 0 View
How can I calculate the pH of internal organelles using a ratiometric pH method? Which formulas should I use after acquiring the fluorescent measurements? I have employed Lysosensor Blue-Yellow...
17 August 2023 4,708 1 View
I have extracted DNA from synovial fluid from a knee and I have an A260/A280 ratio of 4.3 and ds DNA concentration of 1002ug/ml. Is my sample contaminated with something and does anyone have any...
16 August 2023 9,059 1 View
might it be because of lncRNA nature? how can I explain this phenomenon?
12 August 2023 9,742 1 View
Dear RS Community, I am trying to predict folding of chimeric oligopeptides with average length of 40 amino acids. Approximately half of each oligopeptide length comes from one reading frame and...
11 August 2023 7,398 0 View
Hello, I am a fifth-year medical student. I want to initiate research in the field of neuroregeneration after injury. However, I am facing difficulties in identifying a starting point for my...
11 August 2023 3,274 1 View
Can cDNA be checked on 1% agarose gel before qPCR to confirm the quality of the RNA that it was converted from or the efficiency of the RT reaction
09 August 2023 8,315 3 View
I need the full text of the "Determination of dendrobin in dendrobium nobile by HPLC analysis " article. Would you help me with...
07 August 2023 3,234 0 View
Hi everybody, I would like to know which is the acceptability limit of Papp of Lucifer Yellow in assessing Caco-2 cells monolayer integrity. Some authors and Corning manual cite
07 August 2023 863 2 View
Hello all, I'm studying Th-1, Th-2 and Th-17 response in vaccinated and control mice groups. I perform T-cell cytokine Elispot (IFN-gamma, IL-5 and IL-17) on lung and spleen tissues. I've faced an...
01 August 2023 7,920 0 View
Hello, I'm currently optimizing a transcription factor ChIP protocol for a few lymphoma (suspension) cell lines. I'm using the SimpleChIP Enzymatic Kit from CST which incorporates a combination...
01 August 2023 1,800 1 View
I have been trying to optimize the structure of ionic liquid containing ammonium as cation and closo dodecaborane as anion with gaussian and gauss view. But the anion instead of developing...
31 July 2023 7,229 1 View