Cell culture is the complex process by which cells are grown under controlled conditions, generally outside of their natural environment. | Contact experts in Cell Culture to get answers
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Questions related to Cell Culture
Hello everyone, could someone recommend me easy differentiation protocol SH-SY5Y? I found this one: https://www.jove.com/t/53193/differentiation-of-the-sh-sy5y-human-neuroblastoma-cell-line but we...
13 June 2022 5,096 3 View
Hello All, I have been noticing that my cells haven't had the greatest viability. Typically when I passage my cells I see upper 90% in terms of viability and only see a drop in viability 3 days...
13 June 2022 7,192 3 View
Hi everyone! We have a primary mesenchymal stem cell culture, obtained from the adipose tissue of a mouse. After about a week, we started seeing these structures that we are not able to...
11 June 2022 6,353 1 View
Hello, I'm currently trying to optimize SYTOX Green as an extracellular nucleic acid dye for the detection of NETs in vitro. Unfortunately, compared to my positive NETosis control (neutrophils...
07 June 2022 5,827 0 View
Good afternoon everyone, I am struggling with splitting and passaging of SH-SY5Y cells. I feel that most of the time, when I check 2 or 3 days later, I have very limited growth and it takes a...
31 May 2022 9,773 4 View
Usually I let my LMAgarose with cells sit on the bench in the cold room at 4C for 30 minutes to allow the agarose to solidify, and then I put the slides into pre-chilled lysis buffer. Tonight I...
24 May 2022 8,408 0 View
I am recently working on implants. for verify the results of the FEA I need to print them with medical grade Titanium but the problem is according to the limitations here i do not have access to...
22 May 2022 6,523 0 View
We have been using recombinant rat IL-1a in rat cell cultures for years, now we are doing preliminary experiments with murine cell cultures, and we are not seeing the response we would expect. We...
17 May 2022 330 0 View
I am working on tissue culture of an epiphytic orchid. The asymbiotic seed germination was screened in different basal media (MS, 1/2 MS, K-C, G-B5, Mitra, etc.) and later sub-cultured in best...
16 May 2022 3,646 8 View
I need to freeze Mononuclear Bone Marrow cells and thaw them at a later timepoint to analyse them via FACS. To freeze the cells I use 90%FBS and 10%DMSO. But when I thaw the cells (using...
16 May 2022 1,124 5 View
For bioethanol production brewers yeast is used. But which commercial yeast is preferred for higher productivity? Thank you
12 May 2022 2,814 8 View
Hi everyone. I am looking for a PET membrane of poresize 0.4-1 micron with diameter below 25 mm. The same as the transwell or culture insert has. Thank you
12 May 2022 3,919 0 View
What can I do to Tris buffer to maintain the negative charge of the plasma membrane during osmosis? Some things I can play around with are pH, the addition of ions like KCl, NaCl, MgCl2, other...
09 May 2022 1,453 1 View
Has anyone used a protocol for IM injection of tamoxifen to induce local induction of Cre in skeletal muscle?
02 May 2022 1,792 0 View
For measuring whether there is cellular growth occuring or not during syngas fermentation, what is the blank to be used for spectrophotometer?
26 April 2022 8,904 3 View
Hi, I would like to ask, why my reverse transcriptase from fermenter is less active than reverse transcriptase produced from flasks? Thank you for responses Bohuš
25 April 2022 8,731 3 View
Hi, is it possible, that fermentation Can cause low activity of produced Reverse transcriptase? My RT from Erlenmayer flask is more active. But RT from fermenter Was expressed from T5 system and...
19 April 2022 8,374 0 View
Hello, I'm having an issue with EDR, the NUMBER of error is 1212, and I'd appreciate it if you could assist me.
18 April 2022 4,762 0 View
Hello everyone, Currently, I am working on establishing in vitro skin model. I will be reviving HEKa cells and wondering whether the Keratinocyte Growth Kit is important for the survival of the...
11 April 2022 7,695 0 View
Hello friends, We treated SH-SY5Y cells with 50 mM and 100 mM KCL to induce alpha synuclein accumulation. After 24 and 48 hours, we detected the aggregates of alpha synuclein by Zeiss...
09 April 2022 7,097 4 View
Dear colleagues! Recently our trusty plate reader Tecan Sunrise finally died (actually, the light optic channels died, but that seems to be the most expensive part of the device). We have other...
05 April 2022 6,962 4 View
Hello, Can anyone help in determining and explaining how to make 1M of 30% (w/v) H2O2 Hydrogen peroxide? Having a final solution volume to be 300 mL Thanks
30 March 2022 698 2 View
Microwave prepared Hydrogel
29 March 2022 4,037 6 View
Hi all, There are obstacles that I have faced during electrophysiology recording for the neurons derived from stem cells. Neurons were fell out once I started patching cells. I cannot record any...
29 March 2022 456 1 View