Cell culture is the complex process by which cells are grown under controlled conditions, generally outside of their natural environment. | Contact experts in Cell Culture to get answers
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Questions related to Cell Culture
I would like to ask about the dissolution and storage of Camptothecin (HY-16560, MCE) in animal experiments: In rodent (such as mouse/rat) experiments, what solvents are usually used to dissolve...
12 June 2025 6,059 2 View
The cells are transfected first with siRNA, and after 48 hours, I add AngII. After transfection, the confluency goes down, and AngII makes it even worse.
10 June 2025 4,497 2 View
Hello, I would like to ask about the preparation details of the dissolution scheme for animal experiments of Trametinib (HY-10999, MCE): 1. Is 0.5% HPMC (hydroxypropyl methylcellulose) calculated...
10 June 2025 8,338 1 View
I am currently attempting to invert my reporter gene, which is present in an inverted orientation between PhiC31 attB and attP sites. However, when I performed the gene inversion experiment using...
28 May 2025 3,520 3 View
I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...
22 May 2025 2,837 1 View
One of the pathways of degradation of polythene is said to lead via different oxidative mechanisms to carbonyl functions within the polymeric chains. The oxidized chain would then be able to...
19 May 2025 6,991 4 View
I am currently studying the research titled "Development and Validation of a Food and Nutrition Literacy Questionnaire for Chinese Adults (FNLQ-A)" . I would like to ask for clarification...
16 May 2025 6,303 0 View
UV/Vis Spectroscopy and AI
13 May 2025 6,464 3 View
I want to ask for the activity measurement method of BACE1 Protein, Human (HY-P72840). I bought the recombinant protein of MCE. But when I made it myself, the fluorescence signal was very weak....
12 May 2025 7,245 2 View
How to utilize UTAUT model in flipped classroom platform?
11 May 2025 1,619 1 View
I'm planning to characterize my liposome vesicles using Atomic Force Microscopy (AFM). Most research papers recommend using freshly cleaved mica discs due to their atomically flat and clean...
08 May 2025 8,605 5 View
if using probe sonication method for green synthesis of silver nanoparticle what should be the optimized frequency and timing of probe sonication to get silver nanoparticle peak in range of...
05 May 2025 6,531 2 View
So if my yeast strain has a KanMX marker and also has Leu2 post transformation, can I screen both Auxotrophic and dominant markers at the same time on the same plate ex Sc- Leu with G418? If it...
24 April 2025 7,752 3 View
Hi all, I appreciate any help with this question. I am developing alginate beads with a 1.5/2cm diameter containing cells that produce a protein. I am measuring the release of this protein and...
22 April 2025 631 1 View
Hello, I'm trying to create a 12% polyacrylamide gel that can work in both nondenaturing and denaturing conditions, using 7M urea to observe the ssDNA, miRNA, and extended miRNA (polyadenylation...
11 April 2025 5,537 6 View
Looking for a solvent system other than ethanol and DMSO
04 April 2025 972 2 View
i need PhD thesis in management science where smart PLS were applied in that thesis ?
03 April 2025 7,498 0 View
Hello, I am a geologist whose mother-in-law's death has raised many doubts. During a TURB procedure for bladder cancer, a urinary fistula developed. Since the patient passed away six months later...
30 March 2025 7,327 0 View
I've published a preprint https://www.biorxiv.org/content/10.1101/2025.02.11.637722v1 presenting a novel borax-based method for RNA gel electrophoresis. My research shows that borax, surprisingly,...
28 March 2025 863 0 View
handling archived frozen prostate tissue
14 March 2025 377 1 View
I designed an experiment to study the chronic exposure of fish to ivermectin and triclosan. I reviewed the existing literature and discovered that various studies primarily used ethanol and DMSO....
09 March 2025 2,636 0 View
I have synthesized a novel compound incorporating tamarind kernel powder (TKP) and a reagent containing nitrogen and sulfur functional groups. Given the known antioxidant potential of nitrogen-...
04 March 2025 8,697 3 View
In my study, I am working with five different cell lines, two of which share the same genetic features and act as endogenous controls. My goal is to analyze gene expression data using the ΔΔCt...
27 February 2025 1,689 0 View
I'm using the mirvana mirna isolation kit with phenol chloroform. I'm having a difficult time discerning the phase separation upon addition of acid phenol chloroform and centrifugation. I've...
24 February 2025 5,736 1 View