Why I am getting same Ct value in all 10-fold dilutions?

08 May 2024 0 4K Report

I am trying to make a standard curve with 10-fold dilutions of a standard in qPCR. The initial concentration I am using is 60 picomolar which is diluted 10-folds (10:1) for five more dilutions. But in PCR, I am getting same Ct value for all dilutions. Can anyone suggest any modification to get a standard curve please.

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