Cell culture is the complex process by which cells are grown under controlled conditions, generally outside of their natural environment. | Contact experts in Cell Culture to get answers
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Questions related to Cell Culture
A primary difference between photons would be: Absorption/emission events, Hz, and eV associated with sunlight photons Optical photons mostly travel in uG vacuum with less gas RF Photons travel...
05 February 2024 5,456 4 View
hello everyone! HeLa cells were seeded in 6-well plates at 200,000 cells per well. They were left for 2–3 days to reach 70–80% confluency. Then, determination of protein concentration was...
01 February 2024 2,443 5 View
Are there any conditions where it would be possible to lyse gram-negative bacteria using the Promega PLB? I would need to lyse the cells to test for crude enzyme activity but I don’t have a...
31 January 2024 7,592 1 View
I wanted to convert few 2D compounds to 3D using Avogadro and then use them for docking. Are these ligands efficient for docking? Will I get accurate results. Thank you in advance.
30 January 2024 9,695 2 View
Does anyone have a protocol/experience with extracting total protein or RNA from formalin or PFA fixed tissues that have not been paraffin embedded? I want to extract protein/RNA from mouse brains...
29 January 2024 8,915 1 View
From my understanding, the baseline hazard or baseline survival function is unkown because cox regression is semi-parametric model. So why and how can we use it as a prediction model, for example...
29 January 2024 6,911 1 View
I want to conjugate PEG to PLGA nanoparticles.
26 January 2024 1,284 3 View
I am currently doing a final year project on electroplating RNP complexes into Kasumi 1 cells - I have researched all the voltages published in papers and they range from 7500 kV to 90V - I am...
25 January 2024 3,043 2 View
please assist with language editing sites
20 January 2024 9,952 1 View
Hello, I am currently isolating nuclei from adipose tissue and I am interested in obtaining high-quality RNA for further assays. What extraction methods do you recommend? I´m currently using the...
18 January 2024 1,384 2 View
I prepared alginate-tannic acid based hydrogel samples I need to check cell adhesion on hydrogel with DAPI staining and SEM techniques. For SEM observation I need freeze-drying after fixation and...
18 January 2024 3,712 0 View
We want to administer daily injections of VCD in our mice for 20 days. We noticed that VCD (Sigma-Aldrich, 94956) must be stored under inert gas, and do not have a setup for this right now. The...
05 January 2024 9,280 0 View
I am looking for answers as to whether NaHCO3 buffered cell culture media stored outside a CO2 gassed atmosphere changes it's pH irreversibly. I understand how the NaHCO3 buffer principally...
04 January 2024 8,319 3 View
Exploring the potential of edge computing to bolster security measures in Internet of Things (IoT) networks.
04 January 2024 2,553 1 View
I have a dried lyophilized sap from a plant called Croton lechleri Mull. Arg. I want to treat cells in different concentrations (groups): 0.01, 1, 2.5, 5, 50, 100 ug/mL. However, I am not sure how...
02 January 2024 8,054 2 View
My article entitled "Exploration and collection of root and tuber crops in East Wollega and Ilu Ababora zones: Rescuing declining genetic resources" is highjacked by some profile in Research gate...
29 December 2023 7,770 3 View
To understand the relationship between change in marginal error and research sample size.
28 December 2023 8,648 3 View
Whether one independent variable in multilevel regression can be the other two independent variables, such as type, token, and type/token ratio
28 December 2023 8,795 4 View
I need to know the style of this paper research.
28 December 2023 6,864 1 View
Currently, I am measuring gamma-secretase activity by lysing mouse brain, obtaining extract, and processing substrate that reacts with gamma-secretase in mouse brain. The measurement is based on...
27 December 2023 6,273 0 View
How to update H-index.
27 December 2023 640 2 View
i am not able to update my citations and h index
27 December 2023 4,866 5 View
How do I add more publications to my ResearchGate account? Frank J. Hoffman
27 December 2023 1,316 2 View
After cabonization and activation, biomass cellulose were transformed to biochar. But when I collected the XRD specturm, a stranger peak at 10° appeared. how could explain this peak.
26 December 2023 5,563 2 View