Cell culture is the complex process by which cells are grown under controlled conditions, generally outside of their natural environment. | Contact experts in Cell Culture to get answers
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Questions related to Cell Culture
I did 16s metagenomic sequencing using Nanopore. Then I analyzed it with EPI2ME. I got a lot of Cyanobacteria. I assume that they are chloroplast not bacteria. My sample is root of Oryza sativa. I...
20 February 2025 6,877 2 View
In order to obtain 1 gm of Tio2 about 3.7ml of TTIV( Titanium isopropoxide precursor having 27.8-28.6% Tio2) was used and also for obtaining 1gm of Zno zinc acetate of 2.7g in 50ml is taken. Both...
26 January 2025 9,039 2 View
about food related like preservation techniques,new packaging materials
23 January 2025 1,029 3 View
I want to start making sections of the SAN of the heart of mice. Anyone having experience with this? Thanks in advance!
22 January 2025 6,074 3 View
I am using the PCR based methods to tag Saccharomyces Cerevisiae strains with antibody and fluorescent epitopes derived from plasmids. This method takes advantage of Yeast's homologous...
16 January 2025 8,636 3 View
Hello all, I am currently culturing immortalized Human Endometrial Stromal Cells (T-HESC, ATCC). The original ampule I received was at passage 13, and I am now at passage 15. I would like to know...
14 January 2025 3,475 2 View
I noticed that spheroids vary depending on the cell type. When I seed the same number of cells for different types, the resulting spheroids are of different sizes. I need to understand whether the...
13 January 2025 7,561 8 View
Anything helps!
09 January 2025 2,470 1 View
Hi there! HAPPY NEW YEAR to all! I'm having some problems in thy to make special stain to show mast cells in freshwater fishes... See, here we use toluidine blu and PAS to stain the histological...
09 January 2025 5,489 2 View
peak is showing symmetry of 1.9.tailing can be seen clearly. The molecule is a weak acid and a polyphenolic compound. Column: HyperCloneTM BDS, 5 um, 130A 250X4,6mm Buffer: 0.1%...
07 January 2025 8,083 1 View
It looks strange and doubtful
27 December 2024 3,145 1 View
I am attempting to incorporate two restriction enzyme sites into a plasmid vector (p15A origin PREGFP) using super primer PCR. My desired insert length is 80 bp, containing one restriction sites...
17 December 2024 8,174 0 View
Hi, I am culturing B16-F10 melanoma cells. Usually we passed cells in every 4/5 days. But using same lot (newly thawed), cells are growing very fast and we need to passage every 2 days. Looking...
16 December 2024 1,083 2 View
When learning either a primary or secondary language this process is incremental and advancements are not made in days but in months and years. For example, a group of Japanese students was...
07 December 2024 5,790 5 View
In the normalization method, the formula of the normalized force is shown in the figure. Should the ηpl (marked with the red box in the figure) be calculated based on the initial crack length a0...
07 December 2024 7,392 0 View
If I have data for a time series, for example, forty years ago, for a variable or variables, how can I benefit from it in prediction using deep learning or machine learning?
05 December 2024 4,497 2 View
We recently plan to label GABAB receptor subunit 2 (GB2) using IHC and IF, has anyone used this antibody before: (Anti-GABA B Receptor 2/GABBR2 antibody (ab52248)
04 December 2024 2,265 1 View
I accidentally declined a request for one of my published articles.
28 November 2024 7,646 2 View
I attempted to make calcium alginate beads with varying alginate concentrations, but after removing them from the solution and drying, they stuck to the Petri dish and flattened. How can I obtain...
23 November 2024 2,990 2 View
gestion des obstacles
23 November 2024 9,895 0 View
I have 2 cell lines (Hek-293 and MDA-MB-231) and i have a DNA construct that contains a selection marker (Puro) which I wanted to check cell lysates in G2/M, 1/ should I prepare the viral...
12 November 2024 6,853 0 View
Is this toolkit only an extension for YTK (MoClo Yeast toolkit), does it assume that I have this kit and is based on YTK level 0 fragments? Are there any regular YTK promoters or only the newly...
10 November 2024 6,642 1 View
I am exploring the complex relationship between cultural values, societal norms, and their impact on educational inequality across different regions. Research has shown that cultural factors,...
10 November 2024 1,924 0 View
Hello. I am trying to test my designed peptide binding towards MDA-MB-231 and MCF-7 cell lines. However, I first need to dissolved my peptides as they are in lyophilized form. The suggestion from...
28 October 2024 8,973 1 View