Cell culture is the complex process by which cells are grown under controlled conditions, generally outside of their natural environment. | Contact experts in Cell Culture to get answers
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Questions related to Cell Culture
I want to prepare 5% Peracetic Acid (PAA) from Acetic Acid and Hydrogen Peroxide. Can you please help with the exact recipe? I do not have any PAA test kit. Thanks in advance
28 March 2022 5,199 0 View
I wanted to calculate ic50 of mebendazole against a549 cancer cell lines. I have prepared 10mM main stock in DMSO (completely dissolved) but when I am trying to make 50uM, 25uM, and 10uM working...
24 March 2022 9,746 0 View
All exogenous cause and effect that physiologically are neuroimmunoendocrine triggers of cognitive-dependent response that allostatically cause the body to enter homeostasis by the nature of its...
23 March 2022 2,228 5 View
Hello I need to stimulate murine B cells in my experiment. I used anti-CD40and IgM for activation of B cells. but it did not work. (I need to activate murine B cells to check the expression of...
21 March 2022 163 4 View
Hello, I have some problems to record changes in intensities of GcAMP signal. I inserted my GcAMP lenti viral vector in iPSC-derived cortical neurons. I tried to record the GcAMP signal in these...
14 March 2022 2,484 1 View
Would it be possible to use autoclave instead of steam explosion as physical pretreatment ? if yes then what should be the size of biomass ?
11 March 2022 8,692 4 View
We make polyurethane tubes(by extrusion) for medical devices. Polyurethane material we use has 40% BASO4 and hardness of material is 90 Shore A. The tubes made out of this material stick together...
11 March 2022 7,148 2 View
Hello. I am growing human fibroblasts. Spread 20,000 cells on a 12well plate and spread them. When I was observing how fast the cell saturation was, the border was formed on the 5th day, so the...
09 March 2022 9,749 8 View
I am doing a lot of plaque assays with mostly the same cell line. Recently I noticed that the foci were smaller than usually, barely countable. The assay is performed in the same manner all the...
09 March 2022 6,461 0 View
Hello everyone. I am looking for suggestions for HL60 cell line culture. Our lab is new to suspension cell culture. we bought the cell line from National Center for Cell Sciences (NCCS) Pune. Upon...
08 March 2022 5,175 4 View
Does anyone try to use the Q-PCR method to check the titer of retrovirus (MSCV) using the kit? especially the Q-PCR method. I can't find any product that is suitable for this. Does anyone do this...
08 March 2022 6,105 0 View
Their clinical importance
07 March 2022 6,537 4 View
I derived dendritic cells (Dcs) from THP-1 cells by using GM-CSF, IL-4, TNF-alpha and Ionomycin. However when I check their surface markers expression, only CD80 and CD14 increase; but not CD83....
06 March 2022 2,220 0 View
I would like to recover live cells from organoids embedded in methacrylated collagen hydrogel for further culturing. Any suggestions? Thanks in advance
01 March 2022 3,033 0 View
I need to prepare DMEM-HG to culture MDA-MB-231. Besides, 10% of FBS 1% ATB, and the powder formulation, what else do I need to add to the medium?
28 February 2022 7,957 0 View
I am super confused right now about the seeding density for fibroblast. I searched online and most of the company protocol said 4000/cm2. However, based on my own experience, that's clearly not...
24 February 2022 4,938 4 View
Hello All, I am attempting to cleave a purified fusion protein with thrombin. I have assembled the following reaction: 11.5ul Thrombin (550 US Units/mL), 13.2ul fusion protein (19mg/mL), 100ul...
19 February 2022 8,057 0 View
We've bee using morpholino or fully posphorothioated backbone, but I read the initial ASO paper from the 1970s and just straight up DNA was used. I am wondering if I could use these at least to do...
17 February 2022 7,216 2 View
Hi everyone, I have a question regarding Geltrex vs. Matrigel in the differentiation of iPS cells to cardiomyocyte-like cells. So far we have always used Matrigel and obtained high yields of...
11 February 2022 7,959 0 View
Hi, I just started working with breast cancer organoids, and currently using 1ml of freezing medium, which contains 10% of DMSO and 90% of FBS (works perfectly well) to make freeze down. Looking...
11 February 2022 4,253 1 View
My problem is that I have to isolate PBMCs through ficoll manually. and then I use them for positive selection of T lymphocytes by MACS (with anti-CD3 antibody). The only material we have is...
08 February 2022 7,275 1 View
Good morning everyone, I've been trying to keep HepG2 cells in colture for weeks now but they keep dying and I've no idea why. We use: - Minimum essential Medium-Eagle EBSS, with L–Glutamine,...
03 February 2022 170 3 View
Hey everyone, I have been trying to grow 2 types of well-established triple negative breast cancer organoids but I don't see any change in size 20 days post-thawing. I tried using someone else's...
03 February 2022 2,621 0 View
I have noticed these black geometric entities in my lymphoblastoid cell line. They are growing slowly. The cells seem to coexist with them just fine. https://youtu.be/BbrJfgoSm4I Is it hyphae? It...
02 February 2022 431 8 View