Cell culture is the complex process by which cells are grown under controlled conditions, generally outside of their natural environment. | Contact experts in Cell Culture to get answers
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Questions related to Cell Culture
Hi, we work with a rare cancer, adenoid cystic carcinoma. We are in a bind with a shortage of an essesntial cell culture component, the Lonza BBE, CC-4098. Has anyone tried other sources and...
31 January 2022 4,776 1 View
Hi all, I ran into a problem where if I smash the thymus on a petri dish first, then filtering them through a cell strainer, I would get better quality of cell pellets. However, when I smash my...
31 January 2022 3,565 1 View
Dear all, I plan to test the internalization of an antibody on a panel of human cells. However, the supplier added sodium azide to it, which inhibits internalization. The final NaN3 concentration...
28 January 2022 5,847 3 View
Hello, I want to use Propofol, highly hydrophobic, in my cell culture media for human brain microvascular endothelial cells (HBMEC). My stock propofol is 10 mg/ml (Mw: 178.271 g/mol) The...
26 January 2022 2,514 3 View
I have human progenitor epithelial keratinocytes that I've used for years from the same company. They usually look like the image in the vendor's webpage, rounded. Recently, after thawing a vial...
26 January 2022 4,425 0 View
DNA Damage Question I am performing Phospho-yH2AX immunofluorescent staining of cells treated with either 0.1% DMSO or a drug inhibitor in order to measure DNA damage. Unexpectedly, I am...
25 January 2022 1,083 3 View
I'm interested in RyR phosphorylation state and I have specific antibodies to measure the ratio phosphoRyR/total RyR in PBMC but because I'm planning to measure it by flow cytometry I need a...
25 January 2022 5,294 2 View
I have a protein that is easy to aggregate when the temperature is lower than 4℃ or the concentration is more than 5 mg/ml. As we know, protein often is stored on ice or 4℃ during experiments....
24 January 2022 7,779 7 View
Dear all, I'm looking for the best method for characterization of murine extracellular vesicles. It seems that the PS CaptureTM Exosome ELISA Kit (Streptavidin HRP) works well for humain EV but I...
24 January 2022 6,035 0 View
using fluorescence intensity? using ImageJ or any other software?
24 January 2022 3,263 3 View
Good morning everybody, I'm looking for help to identify strange ?contaminants? in my bone marrow MSC cell culture (P1). I do precise that cells were bought and unfrozen in T25 flask. Here is...
19 January 2022 5,123 3 View
Hi all, It was my first time to passage H9 cultured on iMatrix-511(laminin 511) coated wells/dish under feeder-free system, and I found that most colonies were not detached from the well after...
18 January 2022 1,593 1 View
I am culturing suspension cells (Jurkats) and they are in 96 well plate ('v' bottom). I have been noticing a lot of cells (>50%) being lost despite centrifuging them and aspirating the media from...
17 January 2022 7,988 6 View
I am working with primary human fibroblasts from patients with severe metabolic disease. I am interested in using RNA/mRNA re-programming of these fibroblasts in order to obtain iPSCs for...
17 January 2022 3,727 3 View
I am treating cells with water-soluble cholesterol. (https://www.sigmaaldrich.com/SG/en/product/sigma/c4951) Most papers recommend 1-10mM. Upon treatment of cells in medium, even within 10...
13 January 2022 2,408 3 View
Hello, I was wondering why there might be cloudiness in THP-1 suspension cell cultures. The flasks turned relatively opaque after about two days of thawing, and are pictured below at five days...
11 January 2022 3,415 6 View
I am interested in studying priming-associated gene expression in naive human CD8 T cells. I would like to find the best conditions for their short-term in vitro activation, so that their priming...
11 January 2022 2,956 2 View
Testing for proteic nature of a putative bacteriocin: I used 1mg/ml Proteinase K to digest the putative bacteriocine in a cell free supernatent (with unknown concentration, but the supernatant...
11 January 2022 4,724 10 View
HEK293F cells grow in suspension culture and need constant shaking for growth that requires a CO2 incubator equipped with shaker. Has anyone tried alternative ways to culture these cells when...
09 January 2022 6,484 4 View
Hello everyone, I recently found small white dots around the cells as shown in Fig01 and Fig02 while culturing HeLa cells. The 60X magnification is shown in Fig03 and Fig04. It seems that most...
07 January 2022 789 13 View
I have a imidazole compound that gets dissolved in DMSO, Ethanol and in PBS. But at the time of introduction to the cell culture medium, It forms a icy crystal shaped precipitates. How to...
07 January 2022 3,389 4 View
Hi all, We've recently noticed some small bits of black debris floating in some of our cell cultures. As they have popped up in a few different cultures one by one, we've assumed this is a fungal...
07 January 2022 4,938 5 View
Hello! I am going to dissolve phenylalanine and phenylpyruvic acid in the medium. It is a very small amount of about 2mg, so there is a lot of loss when weighing it. Then, I want to make a...
05 January 2022 9,913 4 View
Hello everybody my question is a methodological one: I would like to evaluate CXCL8 at intracellular levels. I have already evaluated CXCL8 levels in cells culture supernatants after 24 hours...
05 January 2022 9,484 3 View