Cell culture is the complex process by which cells are grown under controlled conditions, generally outside of their natural environment. | Contact experts in Cell Culture to get answers
2,478 views 5,044 posts
Questions related to Cell Culture
I was having a discussion with a friend about the best way to resuspend a cell pellet, flicking the tube or pippeting up and down. I wanted to bring the discussion to this community and ask what...
22 December 2023 4,283 1 View
I have a set of experiments ran in triplicate and I have some data points that seems like an outliers in each one? Which method is the best way to examine that? i would appreciate recommendations
22 December 2023 6,747 2 View
The other groups are same in both the material. The solvent system used is also same.
21 December 2023 1,026 1 View
I am conducting a research and would like to know how to get permission to use a research tool seen on research gate.
19 December 2023 241 1 View
[email protected] Dear Prof. Fariha Kanwal I hope this letter finds you well. It is my honor to extend to you an invitation to speak at our upcoming scholar's conference for researchers and...
17 December 2023 4,866 66 View
Hi ResearchGate community, can anyone help us out with this contamination? We work with iPSC-NPC. This contamination happens ONLY when we put the neuronal progenitors in coverslips + media for...
15 December 2023 6,410 0 View
It should be noted that the synthesized particle has a blue emission
15 December 2023 5,123 2 View
Thank you
15 December 2023 2,336 1 View
I have tried to find it online but couldn't.
14 December 2023 454 1 View
I have used expired LS columns (2 years after expiration date) and the results have not been so good. I think that the reasons could be my low number of cells and my tissue, but the expired...
14 December 2023 964 0 View
Hello! I am investigating the extrudability of our bio-ink through rheology. Previously, I used a parallel plate to acquire the shear rate of the sample after which I tried an actual...
13 December 2023 9,484 6 View
Dear ResearchGate community, I am currently a master's degree student at Iran University of Medical Sciences, Department of Biotechnology, embarking on my first-ever exploration of aging and...
12 December 2023 9,463 0 View
i use 0.05% EDTA-trypsin to detach Hep G2 cells 6min then neutralize the trypsin- EDTA with DMEM(HG) with 10% FBS, 1% p/s, and 1(NEAA) and centrifuge gently then resuspend cells in fresh...
11 December 2023 348 5 View
Hi, I will be culturing BM-MSCs in DMEM culture media, based on the literature, I will add 10% FBS and pen/strep... except when I checked the one that we have, I found that we only have...
11 December 2023 6,642 3 View
I was wondering if you know of any online tools for analyzing RNA-seq results? Thanks in advance!
08 December 2023 5,760 3 View
I am working through the challenges of cell isolation from the rat heart. I am using VSMC media and endothelial cell media. In a homogeneous mixture of cells, would these media favor only the...
07 December 2023 4,962 1 View
we are analyzing activity of an endolysin which has N-terminal domain involved in antibacterial activity. The recombinant protein is not showing activity, and we are wondering that whether His-tag...
07 December 2023 8,789 6 View
Hello everyone, I wanted to know how do these two networks differ from one another? Are DNs a subgroup of IPNS, or are they completely different? Thank you in advance.
07 December 2023 9,811 2 View
On the cell surface, there are some circle-like bubbles. Is this contamination? I want to know about that. Thank you.
06 December 2023 8,402 2 View
I am having a doubt regarding CMV promoter and SV40 promoter in mammalian expression vector 1. Why CMV and SV40 promoters are used in mammalian expression vectors why not others? 2. There is a CMV...
06 December 2023 1,794 3 View
IHC
03 December 2023 6,337 1 View
Hi everyone, I got a question about the methods/assays I can use to detect Riboflavin inside the cells. Also after treatment with RF ? Fluorescence spectroscopy, HPLC, ELISA, seahorse??? If...
03 December 2023 2,972 0 View
What is the myth of sun charged water and what kind of electromagnetic waves does the Sun have?
01 December 2023 1,041 2 View
Does sun have infinite energy and what zone in the Sun’s interior transport energy by electromagnetic waves?
01 December 2023 8,365 5 View