How to decide on annealing temperature for primer pairs in RT-qPCR?

Hello i am trying a pair of new primers. Unfortunately I do not have enough reactions to perform a temperature gradient. My HK primer has Tm of 60.3 (forward) and 60.8 (reverse) and the other...

05 March 2019 186 7 View

Is there a min amount of cells per samples in ROMO1 ELISA?

Hello everyone, I am pretty new to ELISA and I have been thinking of conducting a ROMO1 quantitative sandwich ELISA with NHDFa. I am planning on having three samples in triplicates however in our...

21 November 2018 3,956 2 View

I will buy the LSD1 Inhibitor Screening Assay Kit. Could you suggest me a kit or a brand?

I am planning to buy a LSD1 Inhibitor Screening Assay Kit. I do not have much experience with this. Is it more advantageous to buy the enzyme separately or to take it with the kit? Is there anyone...

29 June 2018 6,105 2 View

How can I prepare AESO as a resin for composite production ?

... in terms of initiator, accelerator, hardener additions and their ratios

16 February 2018 5,454 3 View

Do you know any place or website to get Lymantria dispar eggs?

I need Lymantria dispar larvae for my thesis. Do you know any website that sells or any lab that produces Lymantria? Thanks.

01 April 2015 5,900 17 View

Is there anyone who works with Bacteriorhodopsin?

24 September 2014 3,565 4 View

What is the pH effect on UV spectra?

Could you please give me some information about the pH effect on the UV spectra? Is there a common tendency for pH changes or does it depend on the molecule which it is measured? Thanks in advance.

07 September 2014 6,970 15 View

From particles number to concentration - can anyone help?

02 September 2014 6,750 19 View

No title

I would like to know how you guys use AIBN for cross linking? Should I just mix a certain amount of the monomer and AIBN and let them interact at a specific temperature? What are the tips for the...

09 April 2014 8,152 4 View

What are the reasons that polymers (not conducting) interact with double stranded DNA while not interacings with single stranded DNA?

Polymer and DNA interaction

08 January 2014 7,628 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

What is the proper in vitro culturing method to get information about the activation of innate immune cells by VLPs?

Dear All, I am interested in studying the response of immune cells against virus-like particles (VLPs). I used the PBMC in vitro assay and treat it with the VLP using PHA as a positive ctrl while...

16 February 2021 1,337 2 View

Why does flow deformation occur at the test line and control line on nitrocellulose membranes (LFIA)?

I use 1% BSA in 10 mM phosphate buffer (pH 7.4) solution when striping my nitrocellulose membranes with antigens and antibodies. However, I always observe deformed flow when my sample or running...

16 February 2021 4,224 3 View

Can the viability assays (ex: MTT/CCK-8) measure cell proliferation? or are they solely viability assays?

I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...

14 February 2021 3,142 7 View

Is there research limitation on the relationships between digital capabilities, market orientation, brand perception and business performance?

I am looking for supporting studies discussing literature limitations on the relationships between digital capabilities, market orientation, brand perception and business performance. Also, a...

14 February 2021 7,457 5 View

GST assay in microplate?

I'd like to know if anyone can provide me with a protocol for traditional GST enzymatic assay (with GSH and CDNB as substrates) in a 96 well microplate, for cell line and/or C.elegans...

11 February 2021 1,328 3 View

What lysis buffer is compatible with Bradford assay?

Hello! I am planning to use Bradford assay to measure protein content in PBMCs lysates. The problem is that Bradford assay doesn't seem to be compatible with Tris-based buffers or SDS. Are there...

10 February 2021 1,797 5 View