How do I interpret fit statistics SEM and Modindices output?

Hi, I would need some help in understanding the SEM output. I run a latent change score analysis to test the change score of a variable of interest at two-time points (repeated measure) PLD1 and...

05 January 2021 3,367 2 View

Lipofectamine 3000 in 10 cm dish?

Dear all, I'm trying to transfect HeLa cells with lipofectamine 3000 in a 10 cm dish. Do someone of you have a protocol to share? I still examined the table for scaling up or down lipo3000 but it...

13 October 2020 2,926 3 View

Which is the best system to keep endocrinology samples frozen in the field, without electricity?

Hi everyone, I am looking for suggestions to store biological samples in the field for endocrinology analyses, where I will not have electricity. One of the options we are evaluating is a solar...

01 September 2020 3,766 3 View

How to combine beam and shell elements in ANSYS APDL?

Hi everyone! I have a doubt and I hope there is someone who can give me an advice. I am modeling a MEMS gyroscope for my Master thesis and I am starting with a very basic structural design. My...

20 August 2020 9,524 5 View

How to edit density and heat capacity in Aspen Plus ?

Recently I'm trying to edit a pure component mass density and heat capacity of an existing component NaNO3 but failed. Could anyone help me on this? Thanks a lot.

17 July 2020 8,800 8 View

Do you have suggestions about CROSS CULTURAL ADAPTATION of questionnaires?

Hi! I am currently translating and adaptating a questionnarire about attitudes towards inclusion, and I would like to have suggestions about specific literature in translation and cross-cultural...

23 March 2020 2,630 3 View

Can the pcDNA5FRT/TO vector be used for transient overexpression of a gene in a common cell line?

01 March 2020 3,206 2 View

Does CXCL8 or other chemokines induce in vitro cell proliferation?

hi everybody, i have some troubles with chemokines and cell proliferation. Different papers evidence that CXCL8 (but also CXCL12, CCL2 and other chemokines) are able to promote cancer cell...

03 February 2020 214 4 View

Anyone could suggest how to analyze calcium imaging using imagej?

Hi, we would like to analyze time sequences images of calcium imaging assay performed with Fluo4-AM in imagej. Is there any plugin that we could install to do this? Do you have any kind of...

27 January 2020 2,665 1 View

Chloroform and isopropanol RNA extractions?

I have to perform RNA extraction with TRIzol LS and I just realized that we have chloroform stabilized with ethanol. Is that ok? And should chloroform and isopropanol be "molecular biology...

30 September 2019 4,647 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View