I want to design a retina from stem cell, can anybody know about this?

More Kestane Sevil's questions See All

How can I digested Uterus tissue removed from -80 C ?

I have to do a culture from Uterus tissue, thank's

06 February 2024 5,756 0 View

How can I calculate stem cell numbers with a thoma cell counting?

I counted a total of 600 cells in one area of the thoma counter

26 December 2023 8,241 1 View

I want to determine the hematopoietic stem cells in the human blood circulation, which method can I determine it, other than flowcytometri?

We usually look at the flowcytometry method, but this device is not available in my university.

09 July 2023 8,661 4 View

I want to make a retina organoid from rat; can anyone help me about it?

16 December 2022 436 4 View

Can anyone can please help me for Simultaneous Multiple Regression?

Anyone knows how to calculate the change in R squared for each variable doing simultaneous multiple regression analysis? I have 6 independent variables and i want to know the amount of variance...

20 May 2018 505 5 View

Would you please explain the pros and cons of TENS?

TENS = Transcutaneous Electrical Nerve Stimulation

19 September 2016 8,124 3 View

Is anyone interested in collaborating in a cross cultural study about menopause?

Thanks in advance for your replies.

27 December 2014 7,797 2 View

Brain Endurance Training

Dear friends, I want to work on brain endurance exercise and I need help with this. This topic caught my attention. How can I use this to direct medical students to sports?

01 January 1970 7,321 2 View

What are the emerging trends and opportunities for STEM education and careers in the online space?

10 August 2024 507 0 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

01 August 2024 7,677 2 View

Best mask design for metal deposition aiming build a Perovskite solar cell device?

What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?

31 July 2024 4,835 3 View

How to culture and maintain Lx-2 cells?

Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...

31 July 2024 3,893 0 View

How to treat monolayer culture cell line with essential oil?

Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...

31 July 2024 5,301 2 View