How do you prepare McBain medium for oral biofilm formation?

More Roda Seseogullari-Dirihan's questions See All

Have you done nuclei isolation of fixed cells ?

Hello, Have you ever extracted nuclei from fixed samples ? When I fix my HepG2 cells with 1% formaldehyde, I noticed that the fixation prevents cell lysis with regular nuclei isolation buffer,...

03 May 2023 561 5 View

Is it possible to replace letters with codon usage in a promoter sequence?

Hello, I have to synthesize a promoter sequence, but due to Long homopolymer stretches (AAAA...), it is impossible to do it. Can I change the sequence with codon usage without affecting the...

26 January 2022 9,319 0 View

Which are the differences in contact solution Abaqus 6.10 vs 6.14?

Hello all, Some time ago I solved a transient thermo-mechanical problem using Abaqus 6.10, and I was quite satisfied with the observed results (frictional dissipation energy and power loss due to...

28 December 2017 1,153 4 View

Does anyone have experience and suggestions a colchicine induced ploidy level protocol for grass species?

Which extant can give the most success, seeds? Early studies suggest to apply colchicine directly on seeds but why recent studies mostly use also different improved tissues, is there any...

11 October 2014 5,088 1 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

How does social media influence people's dental habits and interest in aesthetic dental treatments?

Hello, Dear friends, I am surveying as part of my thesis research and would greatly appreciate your participation. The study investigates how social media influences dental health behaviors...

01 August 2024 9,349 2 View

IHC profiler in image j software. has anyone used it to quantify nuclear DAB positivity?

Dear researchers. I tried using the IHC PROFILER in image j to quantify nuclear DAB staining. I followed the instructions in the original article by "Varghese F, Bukhari AB, Malhotra R, De A...

29 July 2024 2,229 0 View

How do I correct that wrong author's profile in my publication?

I am writing to request a correction regarding the affiliation of one of my co-authors, Donald Ho. Currently, the author's profile incorrectly lists an affiliation with The Hong Kong Polytechnic...

28 July 2024 6,824 0 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...

22 July 2024 9,576 4 View

Can anyone suggest the specification of the test tube used for the tube adherence method for biofilm assay?

when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.

17 July 2024 3,532 0 View

How do I prepare a working stock solution of 14C sodium bicarbonate to get a final activity of 3uCi?

I need to make a stock solution for radioisotopic incubations of freshwater sediments to study dark primary productivity but I have some confusion as to how I calculate the concentration of 14C...

15 July 2024 5,898 0 View

Isolation of Hydrogen Producing Bacteria?

any good research which can show selective microbial strains for producing hydrogen? Yes the normal procedure is known but the isolation is the key for such selective microbial strains which can...

13 July 2024 2,255 3 View