Hello,
I have been exposing human chondrocytes to LPS (Lipopolysaccharides from E. coli O111:B4) in concentrations from 1 µg/ml to 40 µg/ml for 24 h and 48 h and haven’t seen any decrease in cell viability assessed by MTT. Interestingly, results showed concentration-dependent increase in viability up to 150 %. After consultation with my colleagues, I cultivated cells in medium without antibiotics (previously, I used medium with ATB) but no decrease was observed either.
Do you have any idea why treatment with LPS does not cause cytotoxicity?
Hi, I'm not sure whether the medium is serum free one. If it is not, why don't you try the LPS treatments in the serum free media. Please refer to this paper.
Article MicroRNA‑200b relieves LPS‑induced inflammatory injury by ta...
Besides, further concentration range of LPS treatment could be also evaluated.
https://www.nature.com/articles/s41420-018-0050-9
Especially, regarding the following paper:Article Lipopolysaccharide pretreatment inhibits LPS-induced human u...
, cell viability was increased after treatment with low doses of LPS. Considering the experimental results, it seems to be that you can try lower and higher concentrations vs your previous concentration range of LPS to see what happens.I hope you will solve the problem.
I would suggest treating the cells with a lps for a shorter period ... it could be that after 24 hrs and 48 hrs the cells have recovered from LPS and even show a compensatory response
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