17 November 2023 1 8K Report

I need a detailed protocol of SDS-PAGE only for drosophila if anyone has done before

More Gorthi Vaishnavi's questions See All
Can any of you suggest how to find the detection limit.?

I am working on flourescence sensing of heavy metals using quantum dots.Can any of you suggest how to find the detection limit. Whether it is calculated from formula or we can get from instrument?

19 July 2024 7,411 3 View

Can anyone suggest what amount of powder I have to disperse in the solvent for taking the FTIR spectra?

I have to compare the FTIR of the two samples, one is in powder form and other one is the powder dispersed in IPA or water. Both are same material, so how the bond will change

09 July 2024 8,011 2 View

Whether size obtained from TEM and XRD should be equal?

I have got crystalline size around 15nm from xrd but TEM is giving particle size 4nm

20 June 2024 6,064 8 View

If I dop a metal ion into the ternary nanoparticles can I call it as heterostructure?

Ag+ ion replaced some of the Cu+ ion. ie, Ag+ is the dopant

14 June 2024 2,814 1 View

Whether the same synthesised inorganic nanoparticle show different heavy metal sensing by flourescence?

as

14 June 2024 7,373 1 View

If silver is doped to copper indium sulphide during hydrothermal synthesis by repacing some amount of copper, what will be the size of nanoparticle ?

If I dope silver during the synthesis of copper indium sulphide nanoparticles by hydrothermal method what will be the size of nanoparticles?

13 June 2024 6,844 3 View

What is the size effect of this nanoparticle for flourescence sensing of heavy metals?

Hi, I am working on the flourescence sensing of heavy metal ions in aqeous media using chalcogenide nanoparticles. If I dope a metal ion into this nanoparticles by reducing the moles of already...

11 June 2024 5,568 2 View

Can anyone tell me what's went wrong (granules kind of matter between the cells) of HepG2 culture?

I have used 10% FBS and this image i took after 3 days of passage. and I don't see any cells floating from where i think it might not be contamination but I don't understand what it is. If this is...

18 February 2024 9,843 3 View

What type of microorganism is this?

Upon investigating the microbes present in the spoiled fruit sample, one colony exhibited characteristics reminiscent of water bubbles: shiny, mucoid, and emitting a mild scent. Analysis via...

05 February 2024 6,284 0 View

How to differentiate between dead cells and contamination?

I have revived an adherent cell line from second day I noticed a whitish cloudy layer in media. When i disturb the media the cloudy layer get easily dispersed into the media (more no. of dead...

16 January 2024 7,698 4 View

Similar questions and discussions
Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

How to preform densitometry on SDS-page bands?

I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...

05 August 2024 9,805 3 View

Following click reaction in cell lysates, protein is immobile and remains at the top of the gel in SDS-PAGE?

I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...

29 July 2024 950 4 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View

What is the cause of this aggregation just bellow 70 kDa mark?

Hello, I was running a 12% SDS Page electrophoresis on few granulosa cell samples and got this result after the ponceau staining. The total protein lysate seem to aggregate at 70 kDa ladder mark...

21 July 2024 5,128 4 View

Less substrate signals towards the last lanes of the Native PAGE?

I have been running native page for FAM DNA substrate ( fluorescence samples) for protein DNA binding reaction. Binding is there but towards the end of the lane , I am loosing signals...

17 July 2024 6,213 4 View

Anybody can assist me in Protein and magnetic beads seperation?

Hi, I hope you are doing well, I am working to remove DNA from protein sample by using silica hydroxode magnetic beads. I used different binding buffers but I din't get my protein band in initial...

02 July 2024 5,502 4 View

How accurate is Image J for SDS bands analysis?

I want to check my protein expression but the sample (target protein) on my SDS PAGE shows a little difference as compared to the control. I measured the band density through IMAGE J but the band...

01 July 2024 1,841 3 View

How to troubleshoot error E1 on Biorad Powerpac Basic despite using sufficient and fresh buffer?

I have used this BIorad Powerpac Basic for 4 years now, before which it was used for another 5-6 years. Generally we addressed the E1 error by compensating with fresh buffer and/or filling the...

01 July 2024 7,104 1 View

Thin DNA gel loading sample tips?

I have a 0.25mM thick gel and I am having trouble cleaning out the wells to ensure constant sample application. I usually use a thin syringe to get into the wells and rinse them out with running...

01 July 2024 2,124 2 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close