I want to isolate the cancer stem cells from cancer cell lines. But most of the people are isolating the cancer stem cells by using FACS. But in my lab it is not available. So I want to use another efficient methods to isolate cancer stem cells. I am hopefully expecting effective answers and suitable protocols for that.
Thanking you all.
I'm not in expert in cancer stem cells, but if you have specific antibodies (you would also need for FACS), you can use MACS. Works quite well for isolation of specific cell populations.
But, sir we didn't have FACS. For that reason I am requesting other alternative method.
You don't need a FACS, you just need the Antibodies (and the MACS Kit).
Please see:
http://www.miltenyibiotec.com/en/products-and-services/macs-cell-separation/cell-separation-reagents/cancer-stem-cells.aspx
https://www.miltenyibiotec.com/~/media/Files/Navigation/Research/Stem%20Cell/MACS_Stem_Cell_Research.ashx
Cammareri et al (2008): Isolation and culture of colon cancer stem cells.
You don't need a FACS, you just need the Antibodies (and the MACS Kit).
Please see:
http://www.miltenyibiotec.com/en/products-and-services/macs-cell-separation/cell-separation-reagents/cancer-stem-cells.aspx
https://www.miltenyibiotec.com/~/media/Files/Navigation/Research/Stem%20Cell/MACS_Stem_Cell_Research.ashx
Cammareri et al (2008): Isolation and culture of colon cancer stem cells.
One way researchers enrich for cancer stem cells is by culturing in non-adhereant, sphere forming conditions. By doing so you do not get a pure "stem cell" population but you do enrich for "stem cells" which can be passaged multiple times. Here is a publication on the idea using primary breast tumors.
Hope this helps.
http://cancerres.aacrjournals.org/content/65/13/5506.full.html
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