I am trying to understand whether cancer stem cells are the metastatic cells in cancer progression because of their ability to self-renew and proliferate on a single cell level.
I am trying to preserve a population of glioblastoma cells that I believe to be cancer stem cell. I wish to passage the cells and preserve their "stemness" during each passage. I need the right...
04 May 2016 1,735 2 View
I have tested my glioblastoma U87 cancer cell lines for cancer stem cell markers. I wish to see a way other than stem cell marker expression, the potency of my cells.
31 December 2015 7,085 3 View
I have obtained some glioblastoma cancer stem cells and I want to determine their self-renewal potency based on the colony forming ability. If there is any other assays to determine potency for...
31 December 2015 9,520 2 View
I am trying to separated stem cells from old differentiated senescent to enrich a population of my culture. Stem cells are usually in a quiescent state, so to separate would be ideal for me.
11 December 2015 7,515 5 View
I am culturing this U87 cell line and I was wondering what is the recommended media. I have read the literature, but they have been giving conflicting answers. Also, if there is any extra advice...
09 October 2015 9,222 3 View
I am try to find a way to differentiated the cancer cells to a point where they are terminally differentiated. They are similar to Neural stem cells which is why I removed all of the growth...
09 October 2015 8,486 2 View
In the NSC media I use neurobasal medium (life technology) b27 supplement, 20 ng/mL human FGF-2 (Peprotech), 20 ng/mL human EGF (Promega), and 40 μg/mL heparin (Sigma). Glioblastoma Medium is N2...
07 August 2015 9,375 1 View
What are the emerging trends and opportunities for STEM education and careers in the online space?
10 August 2024 507 0 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...
03 August 2024 5,828 4 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View
What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?
31 July 2024 4,835 3 View
Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...
31 July 2024 3,893 0 View