How can I harvest tumor cells from matrigel coated flask?

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Immunological effect of matrigel on mouse immune system ??

Matrigel is very excellent material for tumor engraftments. We know its positive effects only. Does anyone know about its immunological effect on mouse immune system ? Thanks and regards Anuj

03 April 2019 5,260 0 View

Can anyone share a reference showing slpeen weight of DBA/2 mice ( Naive ) 10-12 week old ?

Hi, I am working with DBA/2 mice and I want to know spleen weight of naive and nulliparour 10-12 week old female DBA/ mice Please help me Regards Anuj

10 November 2017 1,065 0 View

4T1 cell line is contaminated with Mycoplasm ?

I have 4T1 cell line and it is contaminated with Mycoplasma .I passed it through nude mice subcutaneous injection and cultutered tumor cell and tested for mycoplasma presence and found it's...

10 November 2016 9,897 6 View

I want to develop LTPA cell line tumor model?

10 November 2016 8,724 0 View

How much possibility is there that mycoplasma will infect murine tumor cell line ?

If anyone have any adea about the corelatiob between mycoplasma and murine tumor cell line like B16F10, CT26 and 4T1 Anuj

07 August 2016 974 7 View

Hi friends, I want to know about any recent research on topic " How to remove mycoplasma from mycoplasma contaminated cells injected in Nude mice ?

Please provide your inputs on this topic

07 August 2016 973 0 View

Is any link between medium volume, cell growth and gaseous exchange during the culture of cancer cell line ?????

04 May 2016 7,436 7 View

How can I identify graft rejection in female nude mice?

31 December 2015 7,748 1 View

Could anyone tell me that if I inject evans blue dye (20mg/kg-5mL/kg) into lung of mice, will the mice survive or not for at least one week?

Thanks in advance for your replies.

04 May 2015 4,239 4 View

Can any one help me the coordinates to inject the cells for orthotopic GBM Model development using U87 cell line?

Please can you tell me the coordinates ( x, y and z from bregma ) to inject the cells for orthotopic GBM Model development using U87 cell line?

11 December 2014 7,222 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Sila Appak

For the enzymatic methods, I know that dispase would lyse the matrigel. Apart from that some people use ice cold PBS to break the matrigel but I have not tried it myself.

Suman Karki

Cold PBS breaks the matrigel, however it is still tangled after spinning down the cells. I am trying cell recovery solution, let you know how it works.