Hi guys,
I recently developed a HEK293 cell line with an expression reporter at the endogenous locus of a gene interacting with EMT-associated genes. To test the functionality of the reporter based on such interactions, we want to induce EMT in these cells with TGF-Beta1. However, I've looked very hard for previous literature describing EMT in HEK293 cells, specifically induced by TGF-Beta, preferably with morphological data, but I cannot find anything. Does anyone know a good source to address my question? I'm concerned the lack of literature suggests this isn't easy to do.
Thanks!
Kevin
my recollection is that despite their origin, HEK293 cells express N-cadherin and not E-cadherin.
Hi guys, this is an interesting point, we have the same issue. I wonder if HEK293 would go to MET, the opposite, as they express N-cadherin?
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