Which software is better to write systematic review paper?

We have seen some authors use some software to write review paper. As obvious, the study of all published papers concerning our studying field is not possible and these software can help us to...

27 February 2021 6,296 1 View

Reaction condition to convert NH2 to COOH?

I need to couple Dopamine by NH2 end to silica nanoparticles coated by PEG-NH2, but first i need to convert the PEG-NH2 to COOH by using succinic anhydrous ... can you suggest a reaction condition ?

13 February 2021 2,768 4 View

Surface modification of silica nanoparticles with PEG?

I'm trying to coat my silica NP with diamine PEG, so i used GOPTS to add epoxy group on the surface of the NP then i'll add the PEG. I can not found protocol for SiNP coating PEG, most of the...

13 January 2021 4,915 4 View

Silica nanoparticles synthesis using Stober method with size rang 30-50 nm?

I'm preparing silica NP controlling the size by using different concentration of NH4OH. 1- 16.75 ml ethanol. 2-0.5 ml TEOS. 3- NH4OH (0.3- 0.5- 0.75- 1 ml) mixed for 24 hr then evaporate the...

27 November 2020 1,504 13 View

Which neuronal cell line contain D1R?

I'm formulating a drug delivery system that could stimulate Dopamine receptor, For that i need to choose cell line that contain D1R and could be simulated using my delivery system... I have read...

20 November 2020 9,104 2 View

Problems in PCR using Folmer primers, can you help please ?

Hello, I'm using the classic Folmer LCO1490 and HCO2198 primers for a venerid bivalve ADN for two months but the results are still negative, I can't see the bands. 1. I tried to change the...

03 November 2020 9,190 3 View

How to culture prokaryote and eukaryote from the gut of marine organism?

Info regarding the type of media and culturing condition would be really appreciated. Thanks

21 July 2020 6,937 1 View

Calculating sample size for a multivariate regression in GPOWER?

Hello I currently have two dependent variables (with 4 measurements each) and two independent variables My question is how to calculate the sample size using Gpower for multivariate multiple...

21 November 2018 5,719 2 View

CDNA conc. for RT-PCR?

what is the recomended conc. to prepar cDNA to run rt-pcr for brain rat tissue.. i used 50ng/ul some primer gave ct value btw 20-22 but other above 30.. ?

30 October 2017 7,016 3 View

How do i increase the RNA 260/230 ratio ?

i did RNA extraction using differnt protocol triazol and invetrogen RNA mini kit .. the 260/280 ratio was 1.9 to 2.1 but for the 260/230 i got 1.5 to 1.8 in both protocols, should i consider it...

29 October 2017 4,780 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

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02 March 2021 3,060 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View