I try to do the sphere forming assay with oral squamous cell carcinoma cells to test the effect of a drug against cancer stem cells.
I will start a cisplatin treatment soon for NU/NU Nude Mice (Crl:NU-Foxn1nu) and I found multiple publications using 3 mg/kg i.p injection with nude mice. There were different injection frequency...
11 December 2018 5,164 2 View
My article in Medical Oncology is in two places "submission with decision" which is accept and in "submission with production complete". We didn't get any mails from them though. Any one had the...
06 July 2018 5,015 0 View
Tumor sphere formation have been used lately to isolate cancer stem cells and proliferate them. why the non-stem cells don't survive these conditions?
05 June 2018 1,159 5 View
I am asking what is the specific criteria that makes stem cells survive the serum free medium (supplemented with B27, N2, FGF and EGF) and the non-stem cells die?
05 June 2018 7,426 4 View
In cancer stem cells it is always mentioned that they have stemness properties and self-renewal ability. Are those the same thing but different names or there is a difference?
04 May 2018 9,741 4 View
I am trying to avoid the pellet protocol
04 May 2017 7,616 0 View
working on SCC12 cell line where i can get the Matrigel?
07 August 2016 1,203 2 View
What is the best solvent i can use to prepare Floxuridine working solution (will be used on cell lines and animals)? need to get 5 fluorouracil as a chemotherapy for SCC
06 July 2016 5,899 6 View
What are the emerging trends and opportunities for STEM education and careers in the online space?
10 August 2024 507 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View