Why most of the studies use cisplatin i.p injection at dose 3 mg/kg?

I will start a cisplatin treatment soon for NU/NU Nude Mice (Crl:NU-Foxn1nu) and I found multiple publications using 3 mg/kg i.p injection with nude mice. There were different injection frequency...

11 December 2018 5,028 2 View

In Springer publications, my article has two status for two weeks now without any further contacts from them?

My article in Medical Oncology is in two places "submission with decision" which is accept and in "submission with production complete". We didn't get any mails from them though. Any one had the...

06 July 2018 4,886 0 View

Why suspension culture and serum free conditions are used to isolate cancer stem cells?

Tumor sphere formation have been used lately to isolate cancer stem cells and proliferate them. why the non-stem cells don't survive these conditions?

05 June 2018 979 5 View

Why serum free conditions are used to isolate cancer stem cells?

I am asking what is the specific criteria that makes stem cells survive the serum free medium (supplemented with B27, N2, FGF and EGF) and the non-stem cells die?

05 June 2018 7,182 4 View

What is the difference between stemness and self-renewal?

In cancer stem cells it is always mentioned that they have stemness properties and self-renewal ability. Are those the same thing but different names or there is a difference?

04 May 2018 9,544 4 View

Anyone has a protocol for chondrogenic differentiation of MSCs in monolayer ? and a method of quantification of the results?

I am trying to avoid the pellet protocol

04 May 2017 7,481 0 View

I need to do the Transwell migration and invasion assay on squamous cell carcinoma cell line. anyone know the protocol and where can i get the plates?

working on SCC12 cell line where i can get the Matrigel?

07 August 2016 1,023 2 View

What is the best solvent i can use to prepare Floxuridine working solution (will be used on cell lines and animals)?

What is the best solvent i can use to prepare Floxuridine working solution (will be used on cell lines and animals)? need to get 5 fluorouracil as a chemotherapy for SCC

06 July 2016 5,772 6 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View