I am treating gold nanoparticles to cells(cancer cells) and see the adhesion & effect of gold nanoparticles on cells.
When I look at microscope, I find out that gold nanoparticles are aggregated around the cancer cells.
I know that gold nanoparticles aggregate when they are not in DI water, so just injecting the gold nanoparticles to PBS or HBSS will cause the aggregation.
But when I read articles about gold nanoparticles/cells, material & methods just tells me that "AuNPs were incubated with cells for 24h".
Does this mean that they just inject gold nanoparticles on cell media? I think it will cause quick aggregation and the results will not be accurate.
Is there any way I can prevent aggregation of gold nanoparticles treated on cells? (nanoparticles without pegylation. I want to know just buffer change or something.)
Have you tried incubation of GNPs with growth media prior to cell treatment? It may not fully prevent aggregation but protein corona will be formed around the particles, and corona composition affects interaction with cell membrane.
Have you tried incubation of GNPs with growth media prior to cell treatment? It may not fully prevent aggregation but protein corona will be formed around the particles, and corona composition affects interaction with cell membrane.
Yes, you just need to add GNPs to the media without adding anything else.
Have a look at these links
Article Dual Acting Polymeric Nano-Aggregates for Liver Cancer Therapy
Article Combined Effect of Anticancer Agents and Cytochrome C Decora...
- Badges
- Science topic
- Similar topics
- Medicine
- Oncology
- Cancer Research
- Cancer Biology
- Cancer Cells