Could you be more specific on what the protocol will be used for? There are several, but they vary depending on your assay needs. For example, do you need RNA? Are you trying to isolate proteins or immune cells?
i want to isolate protein from nuclei and cytoplasm of WAT and BAT. I need a fractionation protocol to seperate nuclei and cytoplasm and want to do a western blot with this samples. The problem is that with the protocol we use right now (which works nice for liver samples) we do not get enough protein (especially for the nuclei fraction) for WAT/BAT and we would need an adapted protocol to get a higher yield of protein and to get rid of lipids.