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How do i prevent precipitation of organic azide group in azide-alkyne cycloaddition bioconjugation?

Hello eveyone, I'm trying to conjugate azide-MMAE compound to a protein. The azide-containg group is soluble in DMSO. The problem is when i combine all the ingridients for the reaction i get...

08 June 2023 7,244 1 View

How to break propargyl-lysin (PrK) lnduced dimers in the crude fraction?

Hello, I'm working on nanobodies that incorporate propargyl-lysin (PrK) in its sequence. I observed that most of my protein was in dimer form prior to loading it to the antibody column, and that...

15 May 2023 8,313 3 View

A question regarding the ultracentrifugation method for exosomes isolation?

Hi, In the ultracentrifugation method, there are several steps in which one collects the supernatant ,discard the tube with the sediment, transfer the supernatant into a new tube for a higher RCF...

14 November 2021 182 3 View

Comics and its Biblical origins

There might be a chance for Captain Marvel or Shazam (DC Superhero figure - https://en.wikipedia.org/wiki/Captain_Marvel_(DC_Comics)) background story, even if not intentionally, to be related to...

01 January 1970 700 0 View

Theory of relativity and its connection with the Theory of Colours by Gothe

Dear all, I would be happy to know your opinion about whether or not the relationship between theory of relativity and the theory of colours is possible (given that the wavelength of light is not...

01 January 1970 5,783 2 View

If there is a professional misconduct

I would like to propose a certain amendment to the way a lawsuit is conducted against a psychologist in general. When a psychologist is sued for a lack of ethics and professionalism, he should be...

01 January 1970 6,358 1 View

When and how AI is going to make a significant progress in terms of quantum?

How long can it take for GAN (Generative adversarial networks), given its current state of research with regard to its state of the art development, to yield more efficient results in terms of NLP...

01 January 1970 6,945 3 View

Relationship between COVID-19 and cancer

Is there a chance for cancer research fields, such as exponential growth of infected blood cells methodology, to be related to COVID-19 research generally?

01 January 1970 9,299 8 View

Is it right to convict in the absence of a defense?

Dear Colleagues, Why, in the absence of a defense on the part of the accused, is an indictment filed against them accepted as true (and then they are convicted)? It is necessary to distinguish...

01 January 1970 1,862 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Radiogenomics Cancer Research Challenges?

what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...

03 August 2024 5,828 4 View

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