Dear research helpers
We are looking for a protein that is present in the nucleus of (each) human cells. The ideal protein is present with a few copies in the nucleus. Do you know of a protein that matches this description and a specific antibody against that protein is (commercially) available, please let me know. We would like to use this antibody in immune fluorescence microscopy.
I don't do much with nuclear IF besides dapi but I think lamin A/C is a standard control:
https://www.thermofisher.com/antibody/product/Lamin-A-C-Antibody-clone-mab636-Monoclonal/MA3-1000
Is there any reason you can't use a nuclear dye such as DAPI, Hoescht, TO-PRO-3 etc.? They are much cheaper than antibodies and far easier to stain with. Otherwise if you can't use one of them histones are generally a good choice.
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