I want to know the experimental and technical differences between the breast cancer cell lines SUM159 and MDA-MB-231 cells. I wonder, do they have efficiency in sphere-formation( Stemness).
please help me with this.
Thank you
George Sflomos Thank you, Sir, for sharing valuable information. It helps me a lot
mda mb 231 is triple negative breast cancer cel line, i have made 3D spheroids. no problem. fast growing. it will take 48-72 for 3D structure.
seed 500-5000 cells per drop in 20uL.
use hanging drop method
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